培养方式对纤细裸藻脂肪酸与氨基酸含量的影响

EFFECTS OF CULTURE MODE ON FATTY ACIDS AND AMINO ACIDS IN EUGLENA GRACILIS

  • 摘要: 以纤细裸藻(Euglena gracilis)为实验对象, 研究了培养方式对纤细裸藻生长、脂肪酸、氨基酸的影响, 并探讨了可能的作用机理。结果表明, 与其他培养方式相比, 光诱导可提高纤细裸藻总脂肪酸、单不饱和脂肪酸(MUFA)和多不饱和脂肪酸(PUFA)含量, 分别为2.69、0.52和1.475 g/100 g; 饱和脂肪酸(SFA)含量由高到低依次为异养组、光诱导组、自养组和兼养组, 其中异养组含量达1.008 g/100 g; 游离氨基酸含量由高到低依次为光诱导组、兼养组、异养组和自养组, 分别为381.57、358.1、330.17和231.1 mg/g; 兼养组必需氨基酸含量最高, 为134.37 mg/g。实验结果说明光诱导培养可显著提高纤细裸藻总脂肪酸、MUFA和PUFA含量(P<0.05); 异养培养可显著提高纤细裸藻饱和脂肪酸含量(P<0.05); 兼养培养可显著提高纤细裸藻必需氨基酸含量(P<0.05)。研究结果为阐明纤细裸藻对不同培养方式的响应提供了科学依据, 同时为其开发应用提供数据支持。

     

    Abstract: In this research, the effects of culture methods on the growth, fatty acids and amino acids of Euglena gracilis under four cultivation modes (autotrophy, mixotrophy, heterotrophy and photoinduction) at the physiological and biochemical levels were studied. The results were as follows: the fatty acid, MUFA and PUFA contents in E. gracilis were higher under photoinduction than under the other modes, measuring 2.69 g/100 g, 0.52 g/100 g and 1.475 g/100 g, respectively. In terms of SFA content, the modes were ordered as heterotrophy>photoinduction>autotrophy>mixotrophy, and the content in the heterotrophic group reached 1.008 g/100 g. The free amino acid contents were ranked from high to low as follows: photoinduced group, mixotrophic group, heterotrophic group and autotrophic group, measuring 381.57 mg/g, 358.1 mg/g, 330.17 mg/g and 231.1 mg/g, respectively. The mixotrophic group had the highest essential amino acid content (134.37 mg/g). The results of the study showed that the fatty acid, MUFA and PUFA contents in E. gracilis were increased by photoinduction, the saturated fatty acid content in E. gracilis was increased by heterotrophic culture, and the essential amino acid content in E. gracilis was increased significantly by mixotrophic culture (P<0.05). The results may provide a scientific basis for elucidating the response of E. gracilis to different culture methods. Furthermore, they provide theoretical support for the development and application of this species.

     

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