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温馨, 卞瑶, 张昕陶, 马跃, 王晨, 王宏伟. 聚果蜈蚣藻的修订——基于形态观察、早期发育及分子序列分析[J]. 水生生物学报, 2021, 45(6): 1331-1340. DOI: 10.7541/2021.2020.157
引用本文: 温馨, 卞瑶, 张昕陶, 马跃, 王晨, 王宏伟. 聚果蜈蚣藻的修订——基于形态观察、早期发育及分子序列分析[J]. 水生生物学报, 2021, 45(6): 1331-1340. DOI: 10.7541/2021.2020.157
WEN Xin, BIAN Yao, ZHANG Xin-Tao, MA Yue, WANG Chen, WANG Hong-Wei. RESEARCH ON THE REVISION OF G. SOROCARPUS LI ET DING BASED ON MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(6): 1331-1340. DOI: 10.7541/2021.2020.157
Citation: WEN Xin, BIAN Yao, ZHANG Xin-Tao, MA Yue, WANG Chen, WANG Hong-Wei. RESEARCH ON THE REVISION OF G. SOROCARPUS LI ET DING BASED ON MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(6): 1331-1340. DOI: 10.7541/2021.2020.157

聚果蜈蚣藻的修订——基于形态观察、早期发育及分子序列分析

RESEARCH ON THE REVISION OF G. SOROCARPUS LI ET DING BASED ON MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES

  • 摘要: 基于分子辅助的形态分类学方法, 将采自山东省青岛市(模式标本产地)的聚果蜈蚣藻(Grateloupia sorocarpus Li et Ding)在蜈蚣藻属中的分类地位进行重新鉴定, 结果表明: (1) 藻体直立, 单生或丛生, 质地黏滑软骨质, 紫红色, 高5—15 cm, 主枝宽1.5—2 mm, 厚约1 mm, 1—2回羽状分枝, 具有扁平或棍棒状的小羽枝, 小枝基部缢缩, 对生、互生或偏生。(2) 雌配子体的生殖结构为典型的Grateloupia型, 果胞枝生殖枝丛主枝和辅助细胞生殖枝丛主枝分别由6个细胞和5个细胞组成(6cpb-5auxb型); 四分孢子体表面散落分布四分孢子囊, 呈十字形分裂。以上形态学特征均与亚洲蜈蚣藻(G. asiatica Kawaguchi et Wang)一致。(3)聚果蜈蚣藻孢子的早期发育类型为“间接盘状体”型, 与亚洲蜈蚣藻一致。(4)基于rbcL基因序列分析结果显示, 本研究的8个聚果蜈蚣藻样本之间无碱基差异, 与产自中国山东青岛和辽宁大连的亚洲蜈蚣藻无碱基差异, 形成独立的进化支, 与产自韩国的亚洲蜈蚣藻碱基差异为2 bp(0.124%), 与产自日本的亚洲蜈蚣藻碱基差异为3 bp(0.248%), 属于种内差异; 基于COⅠ基因序列构建的系统树显示, 本研究的8个聚果蜈蚣藻样本之间无碱基差异, 与产自韩国和日本的亚洲蜈蚣藻无碱基差异, 形成独立的进化支。根据形态学与分子系统学相结合的分类学方法, 证实聚果蜈蚣藻为亚洲蜈蚣藻的同物异名。

     

    Abstract: Grateloupia sorocarpus Li et Ding collected from Qingdao, Shandong Province was re-examined through morphological and early development observation followed by molecular analysis. The results showed that: (1) The thalli were upright, purplish red, single or clustered, up to 5—15 cm high, main branch width 1.5—2 mm, 1 mm thick. The texture was smooth and cartilaginous. The first branch was flat with pinnately branched 1—2 orders. Small plumes with flat or stick shapes. The branches were opposite, alternate or partial and usually constricted or tapered at the base. (2) Male gametophyte were not seen. The reproductive structure of the female gametophyte included carpogonial branch ampullae and auxiliary cell ampullae. The reproductive structure of female gametophyte showed representative Grateloupia-type; Carpogonial branch ampullae and auxiliary cell ampullae were composed of 6 cells and 5 cells (6cpb-5auxb type). Gametophytes dioecious, mature tetrasporangia originated from inner cortex cells and splited into cross. These characteristics were similar with those of G. asiatica Kawaguchi et Wang. (3) The early developmental type of G. sorocarpus Li et Ding were ‘mediate discal type’, consistent with that of G. asiatica. (4) Based on the phylogenetic tree constructed by gene (rbcL) sequences, there was no sequence divergence between the eight samples of G. sorocarpus and G. asiatica from Qingdao and Dalian, which formed an independent monophyletic subclade within the large Grateloupia clade of Halymeniaceae. The rbcL sequences differences between G. sorocarpus and G. asiatica from South Korea were 2 bp (0.124%) and the rbcL sequences differences between G. sorocarpus and G. asiatica from Japan were 3 bp (0.248%), belonging to intraspecific difference. Based on the phylogenetic tree constructed by COⅠ gene, there was no sequence divergence between the eight samples of G. sorocarpus and G. asiatica from Korea and Japan, which formed a single monophyletic subclade. Considering the morphological observations and molecular analysis, G. sorocarpus Li et Ding and G. asiatica Kawaguchi et Wang were proved to be the same species. According to the priority rule, G. sorocarpus Li et Ding was the synonym of G. asiatica.

     

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