COMPARATIVE STUDY ON BIOCHEMICAL CHARACTERISTICS AMONG CONCHOCELIS OF DIFFERENT NEOPORPHYRA HAITANENSIS
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摘要: 为建立坛紫菜(Neoporphyra haitanensis)丝状体新品种选育的生化评价体系, 对比了5个品种/品系丝状体的光合色素和生长指标, 并利用液相色谱-质谱联用技术检测其红藻糖苷、类菌胞素氨基酸(MAAs)和植物激素的含量差异。结果显示, 藻胆蛋白和叶绿素a与净光合放氧速率和相对生长速率呈正相关, 与坛紫菜的高产特性有关。浙东2号(ZD2)、申福2号(SF2)和闽丰1号(MF1)丝状体的藻红蛋白含量较高, 其相对生长速率也较快。红藻糖苷与坛紫菜的耐高温能力有关, 在MF1和MF2中含量较高; 而MAAs与抗紫外辐射有关, 在MF2中含量远高于其他4个品种/品系, 体现了闽丰坛紫菜的耐高温和抗紫外线的南方品种特性。坛紫菜丝状体中发现了异戊烯腺嘌呤、异戊烯腺苷、反式玉米素核苷和芸苔素内酯4种植物激素, 但激素与生产性状的关联性不明确。综上所述, 研究为坛紫菜丝状体的新品种优选和人工培育构建了一套可行的评价指标体系。Abstract: In order to reflect the correlation between the biochemical parameters and traits of Neoporphyra haitanensis conchocelis and establish a biochemical evaluation system for the selection of new varieties, the photosynthetic pigments and growth indicators of the conchocelis of 5 varieties/strains were compared, and the contents of floridosides, mycosporine-like amino-acids (MAAs) and phytohormones were determined by liquid chromatography-mass spectrometry. The results showed that phycobiliprotein and chlorophyll a were positively correlated with the net photosynthetic oxygen release rate and the relative growth rate, and were related to the high-yield characteristics of N. haitanensis. The conchocelis of Zhedong 2 (ZD2), Shenfu 2 (SF2) and Minfeng 1 (MF1) had higher phycoerythrin content and relatively fast growth rate. Floridosides were related to the high-temperature resistance of N. haitanensis. MF1 and MF2 were dominated by isofloridosides, while other varieties/strains were dominated by floridosides. MAAs is related to resistance to ultraviolet radiation. MF1 and MF2 contained high Shinorine content, and the content of MAAs in MF2 was much higher than that of the other 4 varieties/strains, reflecting the high temperature tolerance and UV resistance of the characteristics of southern varieties of Minfeng. Four plant hormones were found in the conchocelis of N. haitanensis, including isoprene adenine, isoprene adenosine, trans-Zeatin nucleoside and brassinolide, but the relationship between the hormones and production traits was not clear. In summary, the parameters of phycobiliprotein, chlorophyll a, floridosides and MAAs are closely related to the different biological characteristics of N. haitanensis. This study constructed a set of feasible evaluation index system for the selection and cultivation of the conchocelis of new varieties of N. haitanensis.
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Keywords:
- Neoporphyra haitanensis /
- Conchocelis /
- Variety (strain) /
- Biochemical trait /
- Metabolite
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图 1 不同品种/品系坛紫菜丝状体净光合放氧速率测定
不同字母表示存在显著差异, P<0.05, n=3; 下同
Figure 1. The net photosynthetic oxygen release rate in conchocelis of different varieties/strains of N. haitanensis
Different lowercases indicate significant differences, P<0.05, n=3. The same applies below
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图 2 不同品种/品系坛紫菜丝状体生长曲线及相对生长速率
A. 生长曲线; B. 16d时的相对生长速率
Figure 2. Growth curves and relative growth rates of conchocelis of different varieties/strains of N. haitanensis
A. the growth curve; B. the relative growth rate of 16d
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图 3 不同品种/品系坛紫菜丝状体中红藻糖苷含量
红藻糖苷(小写字母), 异红藻糖苷(大写字母)
Figure 3. The content of floridosides in conchocelis of different varieties/strains of N. haitanensis
Floridoside (lowercases), isofloridoside (capital letters)
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图 4 不同品种/品系坛紫菜自由丝状体MAAs含量
Shinorine(小写字母), Porphyra-334(大写字母)
Figure 4. The content of MAAs of different varieties/strains of conchocelis of N. haitanensis
Shinorine (lowercases), Porphyra-334 (capital letters)
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图 5 坛紫菜丝状体不同品种/品系间生理指标相关性分析
*P<0.05, 两变量差异显著, **P<0.01, 差异极显著。相关系数r>0, 两变量呈正相关; r的绝对值为0—0.3, 表示基本不相关; 0.3—0.5, 低度相关性; 0.5—0.8, 中度相关性; 0.8—1.0, 高度相关性。PE. 藻红蛋白; PC. 藻蓝蛋白; Chl. a. 叶绿素a; Car. 类胡萝卜素; ZIP. 异戊烯腺嘌呤; IPA. 异戊烯腺苷; TZR. 反式玉米素核苷; BR. 芸苔素内酯
Figure 5. Correlation analysis of physiological indexes among conchocelis of different varieties/strains of N. haitanensis
*P<0.05, significant difference between the two variables, **P<0.01, extremely significant difference. The correlation coefficient r>0, the two variables are positively correlated; The absolute value of r is 0—0.3, indicating basically no correlation. 0.3—0.5, low correlation; 0.5—0.8, moderate correlation; 0.8—1.0, highly correlation. PE. phyloglobin, PC. phylocyanin, Chl. a. chlorophyll a, Car. carotenoids, ZIP. isoprene adenine, IPA. isoprene adenosine, TZR. trans-Zeatin-riboside, BR. brassinolide
表 1 不同品种/品系坛紫菜丝状体光合色素含量
Table 1 The photosynthetic pigments contents of different varieties/strains of conchocelis of N. haitanensis (n=3)
品种/品系
Variety/
Strain藻红蛋白PE (mg/g FW) 藻蓝蛋白PC (mg/g FW) 叶绿素a
Chl. a
(μg/g FW)类胡萝卜素Car
(μg/g FW)浙东1号
ZD13.93±0.11d 1.28±0.04b 147.81±1.80c 75.33±2.43a 浙东2号
ZD26.22±0.14b 1.21±0.03c 156.32±2.52a 48.60±1.46e 申福2号
SF25.50±0.13c 1.02±0.02d 151.19±1.67b 55.87±1.92d 闵丰1号
MF16.93±0.32a 1.35±0.02a 152.39±1.82b 58.60±0.57c 闵丰2号
MF23.42±0.65e 0.63±0.02e 149.81±2.22bc 65.98±1.09b 注: 最小显著性差异法, 同一列的不同字母表示存在显著差异, P<0.05, n=3。下同Note: Least-significant difference, different lowercases in the same column indicate significant differences, P<0.05, n=3. The same applies below 
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表 2 不同品种/品系坛紫菜自由丝状体中植物激素含量
Table 2 The content of plant hormones of different varieties/strains of conchocelis of N. haitanensis
品种/品系Variety/strain 激素含量Plant hormones (ng/g DW) 异戊烯腺嘌呤ZIP 异戊烯腺苷IPA 反式玉米素核苷TZR 芸苔素内酯BR 浙东1号ZD1 0.11±
0.03c0.93±
0.03a457.85±
40.32c748.35±
113.88c浙东2号ZD2 0.10±
0.05c0.64±
0.07b662.52±
48.78a295.49±
41.74c申福2号SF2 0.72±
0.13a0.57±
0.01b552.91±
76.59b55.27±
16.54c闵丰1号MF1 0.64±
0.17a0.45±
0.07c255.70±
14.51d2325.09±
860.18b闵丰2号MF2 0.42±
0.03b0.85±
0.09a311.36±
13.85d6927.66±
305.66a
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