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杨鑫, 黄坤, 胡靖蕊, 曾丽雯, 杨世勇, 武佳韵. 周公河齐口裂腹鱼种群的遗传多样性和遗传结构[J]. 水生生物学报, 2022, 46(11): 1712-1718. DOI: 10.7541/2022.2021.0231
引用本文: 杨鑫, 黄坤, 胡靖蕊, 曾丽雯, 杨世勇, 武佳韵. 周公河齐口裂腹鱼种群的遗传多样性和遗传结构[J]. 水生生物学报, 2022, 46(11): 1712-1718. DOI: 10.7541/2022.2021.0231
YANG Xin, HUANG Kun, HU Jing-Rui, ZENG Li-Wen, YANG Shi-Yong, WU Jia-Yun. PRELIMINARY STUDY ON GENETIC DIVERSITY AND POPULATION STRUCTURE OF SCHIZOTHORAX PRENANTI FROM ZHOUGONG RIVER[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1712-1718. DOI: 10.7541/2022.2021.0231
Citation: YANG Xin, HUANG Kun, HU Jing-Rui, ZENG Li-Wen, YANG Shi-Yong, WU Jia-Yun. PRELIMINARY STUDY ON GENETIC DIVERSITY AND POPULATION STRUCTURE OF SCHIZOTHORAX PRENANTI FROM ZHOUGONG RIVER[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1712-1718. DOI: 10.7541/2022.2021.0231

周公河齐口裂腹鱼种群的遗传多样性和遗传结构

PRELIMINARY STUDY ON GENETIC DIVERSITY AND POPULATION STRUCTURE OF SCHIZOTHORAX PRENANTI FROM ZHOUGONG RIVER

  • 摘要: 为探讨周公河中齐口裂腹鱼(Schizothorax prenanti)的遗传多样性和遗传结构, 沿周公河连续设定6个采样点进行齐口裂腹鱼采集, 在进行基因组DNA提取后以线粒体控制区(D-loop)为分子标记进行种群遗传多样性和遗传结构的评估。结果从63尾齐口裂腹鱼中共检测到32个单倍型, 核苷酸多样性(π)为0.013, 单倍型多样性(h)为0.966。遗传多样性最高的为张家湾种群(PopF, π=0.018, h=1.000), 核苷酸多样性最低的为罗坝种群(PopC, π=0.010, h=0.970), 单倍型多样性最低的为瓦屋山大坝下游种群(PopB, π=0.015, h=0.867)。种群间共享13个单倍型, 多数齐口裂腹鱼种群间的遗传分化水平中等或较低, 仅Pop C和Pop F (Fst=0.195, P<0.01), 种群Pop A 和PopE (Fst=0.158, P<0.01)分化程度较高, 显示各种群间较密切的遗传关系。周公河不同河段齐口裂腹鱼群体的遗传多样性处于较高的水平, 各种群间具有较近的遗传关系。

     

    Abstract: Zhougong River is one of the birthplaces of Schizothorax prenanti. To explore the genetic characteristics including genetic diversity and population structure of S. prenanti from the Zhougong River, 6 continuous sampling locations were set along the Zhougong River for collecting samples. The mitochondrial control area (D-loop) was used as molecular marker to evaluate the genetic diversity and population structure after extraction of total genomic DNA. The results showed that 32 haplotypes were detected from 63 S. prenanti, the total nucleotide diversity (π) was 0.013 and the total haplotype diversity (h) was 0.966. Compare all sampling points, the highest genetic diversity was detected from the Zhangjiawan population (PopF, π=0.018, h=1.000), while the lowest nucleotide diversity was found from the Luoba population (PopC, π=0.010, h=0.970) and the lowest haplotype diversity was found from the downstream population of Wawushan Dam (PopB, π=0.018, h=0.867). There were 13 shared haplotypes and 19 private haplotype were found in the 6 populations, and the shared haplotypes were mainly distributed in PopD and PopE. Except for populations PopC and PopF (Fst=0.195, P<0.01), populations PopA and PopE (Fst=0.158, P<0.01) have a high genetic differentiation, the level of genetic differentiation among most other S. prenanti populations was medium or low, which indicated relatively close genetic relationship among the S. prenanti populations from Zhougong River. In this research, the relatively high level of genetic diversity and close genetic relationships of S. prenanti populations were detected from different ranges of the Zhougong River, which can provide reference data for the protection of special fishes in the Zhougong River.

     

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