环境相关四环素长期暴露对吉富罗非鱼生长性能、肝脏脂肪代谢和抗氧化反应的影响

LONG-TERM ENVIRONMENTAL-RELATED TETRACYCLINE EXPOSURE ON GROWTH PERFORMANCE, HEPATIC LIPID METABOLISM AND ANTIOXIDANT RESPONSES IN GIFT TILAPIA (OREOCHROMIS NILOTICUS)

  • 摘要: 为研究水环境浓度四环素(Tetracycline, TC)长期暴露对鱼类的影响, 实验以吉富罗非鱼(Oreochromis niloticus)为实验模型, 将其暴露于0、400和800 ng/L三种四环素水平下10周。结果表明, 相较于对照组, 四环素暴露显著提高了生长性能, 但增加了肝脏中甘油三酯含量和四环素浓度。脂肪生成基因表达(fasscdaccαsrebp1pparγ)被上调, 但降低了与脂肪分解相关基因的表达(atglhslcpt1pparα)。此外, 6-磷酸葡萄糖脱氢酶(6PGD)、葡萄糖-6-磷酸脱氢酶(G6PD)、异柠檬酸脱氢酶(ICDH)、苹果酸脱氢酶(ME)及脂肪酸合成酶(FAS)的活性显著增加, 但肉碱棕榈酰转移酶1(CPT1)活性则被显著抑制, 这与基因表达相一致。而且, 四环素暴露显著降低过氧化氢酶、总超氧化物歧化酶的活性及总抗氧化能力, 并诱导了鱼类的氧化应激, 导致丙二醛水平显著增加。因此, 与环境相关四环素浓度可促进吉富罗非鱼的生长性能、上调产脂代谢、降低抗氧化能力并诱导脂质过氧化。

     

    Abstract: Tetracycline (TC) is extensively used in animal husbandry, aquaculture, and medical treatment. However, improper use or disposal of TC can increase its concentration in the aquatic environment. The effects of TC exposure on fish growth, oxidative stress, and lipid metabolism have been reported. But, the information on the potential and long-term effects on fish is very limited, especially at the environmentally relevant level. In this study, tilapia was used as an experimental model and exposed to three TC levels of 0 (control), 400, and 800 ng/L for 10 weeks. The results showed that, compared with the control, waterborne TC significantly improved growth performance, but increased triglyceride content and TC concentration in the liver. TC increased the expression of lipogenic genes (fas, scd, accα, srebp1, pparγ) and decreased the expression of lipolytic genes (atgl, hsl, cpt1 and pparα). In addition, these activities of 6-phosphogluconate dehydrogenase (6PGD), glucose 6-phosphate dehydrogenase (G6PD), isocitrate dehydrogenase (ICDH), malic enzyme (ME), and fatty acid synthase (FAS) increased significantly. but the activity of carnitine palmitoyltransferase 1 (CPT1) was significantly inhibited, parallel to gene expression. Moreover, waterborne TC significantly reduced the activities of catalase (CAT), total superoxide dismutase (T-SOD), and total antioxidant capacity (T-AOC), and induced oxidative stress in fish. The mitochondrial damage may be related to this and lead to a significant increase in malondialdehyde levels. Thus, the environmentally relevant TC concentration promoted growth performance, up-regulated lipogenic metabolism, reduced antioxidant capacity, and induced lipid peroxidation in GIFT tilapia.

     

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