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徐思琪, 张世勇, 段永强, 张文平, 王明华, 边文冀, 陈校辉. 17β-雌二醇诱导斑点叉尾鮰雌性化研究[J]. 水生生物学报, 2022, 46(11): 1668-1674. DOI: 10.7541/2022.2021.0254
引用本文: 徐思琪, 张世勇, 段永强, 张文平, 王明华, 边文冀, 陈校辉. 17β-雌二醇诱导斑点叉尾鮰雌性化研究[J]. 水生生物学报, 2022, 46(11): 1668-1674. DOI: 10.7541/2022.2021.0254
XU Si-Qi, ZHANG Shi-Yong, DUAN Yong-Qiang, ZHANG Wen-Ping, WANG Ming-Hua, BIAN Wen-Ji, CHEN Xiao-Hui. FEMINIZATION OF CHANNEL CATFISH INDUCED BY 17β-ESTRADIOL[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1668-1674. DOI: 10.7541/2022.2021.0254
Citation: XU Si-Qi, ZHANG Shi-Yong, DUAN Yong-Qiang, ZHANG Wen-Ping, WANG Ming-Hua, BIAN Wen-Ji, CHEN Xiao-Hui. FEMINIZATION OF CHANNEL CATFISH INDUCED BY 17β-ESTRADIOL[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1668-1674. DOI: 10.7541/2022.2021.0254

17β-雌二醇诱导斑点叉尾鮰雌性化研究

FEMINIZATION OF CHANNEL CATFISH INDUCED BY 17β-ESTRADIOL

  • 摘要: 研究以人工配合饲料为17β-雌二醇(17β-estradiol, 17β-E2)载体, 对斑点叉尾鮰(Ictalurus punctatus)开口仔鱼连续饲喂27d进行雌性性别诱导, 探究斑点叉尾鮰XY雄鱼雌性化的合适17β-E2剂量。60日龄测量并统计各组试验鱼的生长数据、存活率, 解剖观察性腺结构, 结合遗传性别鉴定结果计算性逆转率, 并通过组织切片分析各组试验鱼中XX和XY雌鱼卵巢发育情况。270日龄检测XY雌鱼和对照组雌鱼的鳃、心脏、肝脏、肾脏、卵巢和肌肉等组织中17β-E2含量。研究进一步地采用qRT-PCR检测270日龄雌雄性别分化基因foxl2dmrt1在XX和XY雌鱼卵巢中的表达水平。结果显示: 各组XY雌鱼的比例和卵母细胞数量均随17β-E2剂量的提高而增加, 且核仁数量也随之增多, 而核质比相应减小; 各组XY雌鱼的体重和体长随着17β-E2剂量的提高先增加后减小, 但存活率没有显著性变化; 各组XY雌鱼与对照组雌鱼的鳃、心脏、肝脏、肾脏和肌肉组织中17β-E2含量均无显著性差异, 卵巢中17β-E2含量与添加剂量呈正相关; 雌性性别分化基因foxl2在XY雌鱼中的表达量显著升高, 雄性性别分化基因dmrt1在XY雌鱼中的表达量显著降低。研究建立了17β-E2诱导XY雄性斑点叉尾鮰雌性化方法, 为后续全雄斑点叉尾鮰新品种的培育奠定基础。

     

    Abstract: In order to explore the appropriate method of feminization and 17β-estradiol doses for male channel catfish, artificial compound feed was used as 17β-E2 vector to feed catfish larvae continuously for 27d. The growth data and survival rate of catfish in each group were measured and counted at 60 dahs, the sex reversal rate was also calculated in combination with the genetic sex identification results. Furthermore, the ovarian development of XX and XY females in each group was compared and analyzed based on the H & E staining of ovary tissue paraffin sections. Finally, 17β-E2 contents in gill, heart, liver, kidney, ovary and muscle tissues of XY females in each group were detected and compared with control XX females at 270 dahs. In addition, qRT-PCR was used to detect the expression levels of foxl2 and dmrt1 in XX and XY female ovaries at 270 dahs. The results showed that the proportion of XY females and the number of oocytes in each group were increasing with 17β-E2 doses. The weight and body length of female fish in each group first increased and then decreased with the increase of 17β-E2 doses. The survival rate was no significant in each group. The content of 17β-E2 in ovary was positively correlated with 17β-E2 doses, while no differences were observed in gill, heart, liver, kidney and muscle of female fish. The expression levels of foxl2 gene increased in XY females, while dmrt1 gene decreased in XY females. This study established 17β-E2 induced feminization of XY male channel catfish, and laid a solid foundation for the subsequent cultivation of new male channel catfish varieties.

     

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