“鲻麻风病”病原鳞上碘泡虫全基因组微卫星分布特征

GENOMIC DISTRIBUTION CHARACTERISTICS OF MICROSATELLITES IN MYXOBOLUS EPISQUAMALIS (THE PATHOGEN OF MULLET LEPRIASIS)

  • 摘要: 为探究微卫星在黏孢子虫全基因组中的作用及其在遗传研究中的潜在应用价值, 研究在对“鲻麻风病”病原鳞上碘泡虫进行全基因组测序的基础上, 采用生物信息学方法分析了其微卫星分布特征和规律。通过对基因组完整型微卫星的筛选, 共得到21957个完整型微卫星, 总长311751 bp, 在全基因组中占0.3%。在6种微卫星重复类型中, 单碱基重复类型的微卫星数量最多, 占42.5%, 其次是二碱基 (26.0%)、三碱基 (17.9%)、四碱基 (12.5%)、五碱基 (0.8%) 和六碱基 (0.4%)。得到位于外显子区的微卫星93个, 分别定位于39个基因。GO注释结果显示, 基因中涉及到生物过程的基因数量最多, 其中最显著的条目是胞内细胞器组分。KEGG富集结果显示, 基因共涉及到80条通路, 其中集合管酸分泌物通路最为显著。GO注释和KEGG富集分析结果表明, 鳞上碘泡虫含有微卫星的外显子可能与体内生物代谢过程密切相关。研究获得了黏孢子虫微卫星分布特征及规律方面的基础数据, 将为黏孢子虫微卫星分子标记开发、遗传分析和病害防治提供参考。

     

    Abstract: Microsatellites are efficient and stable genetic markers widely used in the constructing genetic maps, identifying genetic relationships, and analyzing population genetic diversity. However, there is no information on microsatellites in the genome of myxosporean. In this study, we investigated the characteristics and distribution patterns of microsatellites in the whole genome of Myxobolus episquamalis, the pathogen of mullet lepriasis. The total number of complete microsatellites was 21957, with a combined length of 311751 bp, constituting 0.3% of the whole genome size. Among the six microsatellite repeat types, single-base-repeat type had the largest number, accounting for 42.5%, followed by two-bases-repeat type (26%), three-bases-repeat type (17.9%), four-bases-repeat type (12.5%), five-bases-repeat type (0.8%), and six-bases-repeat type (0.4%). Additionally, 93 microsatellites were located in exons corresponding to 39 genes. GO analysis of these genes showed that the most were annotated to biological processes, with the most significant entries being intracellular and organellar components. KEGG analysis showed that these 39 genes were involved in 80 pathways, in which the collecting duct acid secretion pathway was the most significant. The characteristics and distribution patterns of microsatellites in the M. episquamalis genome were most similar to those in Cnidaria, which provided a new evidence supporting the hypothesis that myxosprean originated from Cnidaria. Microsatellites localized in exons may be closely associated with biological metabolic processes in vivo. Our study first acquired the characteristics and distribution patterns of microsatellites of myxosporean, which could provide a valuable reference for developing microsatellite markers and conducting genetic analysis of myxosporean. This knowledge could also aid in the prevention and control of myxosporidiosis.

     

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