虾青素对饲料组胺胁迫下美洲鳗鲡幼鱼肠道健康的影响

ASTAXANTHIN ON INTESTINAL HEALTH OF JUVENILE AMERICAN EEL (ANGUILLA ROSTRATA) UNDER DIETARY HISTAMINE STRESS

  • 摘要: 为研究虾青素对饲料组胺胁迫下美洲鳗鲡幼鱼(Anguilla rostrata)肠道健康的影响, 以初始质量为(25.01±0.17) g的美洲鳗鲡为研究对象, 随机分为对照组(Control组), 高组胺饲料组(H 组, 组胺实测水平为661.86 mg/kg)及其基础上添加4 mg/kg (H+Ast 4组)、8 mg/kg (H+Ast 8组) 虾青素的实验组。在养殖 10 周后, 测定血清二胺氧化酶活性和D-乳酸水平、肠道抗氧化能力、肠道组织形态及炎症因子基因表达量等指标。添加 4和8 mg/kg 虾青素均可降低投喂高组胺饲料美洲鳗鲡幼鱼的血清二胺氧化酶活性和-乳酸水平(P<0.05); 降低肠道丙二醛水平(P<0.05), 提高肠道超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性(P<0.05); 增加肠道绒毛长度和肌层厚度(P<0.05); 降低肠道组胺 H1 受体、NF-κBIL-6TNF-α 基因表达量 (P<0.05), 提高IFN-γ 基因表达量 (P<0.05)。结果表明, 添加 4 mg/kg 虾青素即可缓解饲料组胺对美洲鳗鲡幼鱼肠道健康的损害, 为缓解鳗鲡饲料源胁迫提供技术参考。

     

    Abstract: This trial aimed to investigate the effects of astaxanthin (AST) on serum diamine oxidase activity, D-lactic acid content, intestinal antioxidant capacity, intestinal morphology, and the expression of histamine H1 receptor and pro-inflammatory factor genes in juvenile American eels (Anguilla rostrata) under dietary histamine stress. A total of 400 fish with an initial weight of (25.01±0.17) g were divided into four groups: the control group fed a basal diet, the H group fed a diet with a high level of histamine (662 mg/kg), the H+Ast 4 group fed the high histamine diet with 4 mg/kg AST, and the H+Ast 8 group fed the high histamine diet with 8 mg/kg AST. Each group had four replicates with 25 fish per replicate, and the trial lasted for 10 weeks. The results showed that supplementation with the 4 and 8 mg/kg AST in the high histamine diet significantly decreased diamine oxidase activity and D-lactic acid content in the serum (P<0.05), improved intestinal glutathione peroxidase, superoxide dismutase, and catalase activity (P<0.05), reduced malondialdehyde levels (P<0.05), enhanced intestinal villus length and muscular thickness (P<0.05), and reduced the expression of histamine H1 receptor and pro-inflammatory factor genes (P<0.05). However, compared to the H+Ast 4 group, an 8 mg/kg AST diet did not provide further improvements in these parameters. In conclusion, this study demonstrates that the addition of 4 mg/kg AST can alleviate intestinal health problems of juvenile A. rostrata induced by dietary histamine stress. These results provide valuable insights into the use of AST to mitigate dietary histamine stress and its scientific application in the eel feed.

     

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