一株自絮凝细菌Massilia sp. W12的多相分类鉴定及全基因组分析

POLYPHASIC IDENTIFICATION AND WHOLE-GENOME ANALYSIS OF A SELF-FLOCCULATING BACTERIAL STRAIN, MASSILIA sp. W12

  • 摘要: 生物絮团的功能主体是高度复杂的微生物聚集体, 设计和优化复杂的微生物群落结构和活性对生物絮团性能的提升具有重要意义。研究以筛选自絮凝菌作为生物絮团系统外源强化菌株为目的, 从中华草龟养殖池塘中分离纯化出一株自絮凝菌株, 对其进行多相分类学鉴定确定W12菌株的分类地位, 基因注释分析探讨其作为强化菌株的可行性。W12菌株与Massilia arenosa MC02T相似性最高, 为96.21%, W12菌株与其亲缘关系较近的几株模式菌株的ANI值在71.61%—72.23%, dDDH值在19.6%—22.9%, 远低于判别细菌新种的阈值, 进一步表明W12为一株马赛菌属新种。此外, 全基因组分析发现W12的自絮凝机制可能与喜树脂动胶菌相似, 由胞外多糖和PEP-CTERM蛋白质共同介导, 推测菌株W12通过PHB合成途径Ⅰ涉及PhaA、PhaB和PhaC三种酶来合成PHB。本研究从中华草龟养殖池塘分离到的自絮凝菌株W12是一株马赛菌属新种, W12菌株可以合成PHB, 由胞外多糖和PEP-CTERM蛋白质共同介导其自絮凝, 这些性能与生物絮团的形成息息相关。基因组分析对菌株的潜在应用价值进行挖掘, 以期为生物絮团系统的功能强化提供候选菌株。

     

    Abstract: The functional components of bioflocs are highly complex microbial aggregates, and the design and optimization of these intricate microbial communities are crucial for improving biofloc performance. To screen self-flocculating bacteria as exogenous reinforcement strains for biofloc systems, a self-flocculating strain was isolated and purified from the breeding pond of Chinese grass turtles. Its taxonomic status was identified through polyphasic taxonomy, which included 16S rRNA gene sequencing, phylogeneticanalysis, and whole genome sequence analyses. Strain W12showed the highest similarity with Massilia arenosa MC02T at 96.21%, the ANI values for strain W12 ranged from 71.61%—72.23%, and the dDDH values ranged from 19.6%—22.9% with several type strains of closely related species, which were much lower than the thresholds for discriminating a new bacteria species. This strongly indicates that W12 is a novel species within the genus Massilia. In addition, genome-wide analysis reveales that the self-flocculation mechanism of W12 may is similar to that of the Zoogloea resiniphila, likely mediated by a combination of extracellular polysaccharides and PEP-CTERM proteins. It is hypothesized that strain W12synthesizes PHB through the PHB synthesis pathway I, involving three enzymes: PhaA, PhaB, and PhaC. The discovery of strain W12, a novel species of the genus Massilia isolated from a turtle breeding pond, highlights its ability to synthesize PHB and self-flocculation through extracellular polysaccharides and PEP-CTERM proteins. These properties are significant for bioflocs formation, underscoring the potential application of strains in enhancing the biofloc system.

     

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