荒漠蓝藻中国新记录属——锥顶丝藻(Konicacronema, Gomontiellales)

KONICACRONEMA (GOMONTIELLALES): A NEWLY RECORDED DESERT CYANOBACTERIAL GENUS IN CHINA

  • 摘要: 锥顶丝藻属(Konicacronema)是Machado de Lima等于2020年从巴西半干旱地区卡廷加群落生境的生物结皮中分离鉴定并成立的蓝藻新属。研究从内蒙古达拉特旗库布齐沙漠边缘生物土壤结皮样本中分离纯化得到了3株丝状蓝藻(APE127、APE132及APE190), 这些藻株在形态学上与束鞘丝藻类(Coleofasciculus-like)和微鞘藻类(Microcoleus-like)蓝藻物种相似。基于16S rRNA基因的序列分析表明, APE127/132/190与隆凸锥顶丝藻(Konicacronema protuberans)的序列相似性达98.7%—99.3%, 系统发育分析表明它们与锥顶丝藻属藻株(Konicacronema spp.)聚集并形成支持度良好的进化分支。同时, 藻株与K. protuberans的16S—23S rRNA间隔区序列(ITS) D1—D1′、Box-B及V3螺旋等二级结构较为一致。结合分子序列、细胞形态和生理生态学数据的多相方法分析表明, 新分离纯化的干旱区丝状蓝藻为隆凸锥顶丝藻(K. protuberans), 该蓝藻为中国新记录属种。

     

    Abstract: The genus Konicacronema, established by Machado de Lima et al. in 2020, was initially isolated from biological soil crusts in the semi-arid regions of Brazil (Caatinga community). In this study, we report the isolation and purification of three filamentous cyanobacterial strains: APE127, APE132, and APE190 from desert biocrust samples collected in Dalate County, Inner Mongolia, China. These strains exhibit morphological characteristics similar to Coleofasciculus-like and Microcoleus-like species. Based on 16S rRNA gene sequence analysis, strains APE127, APE132, and APE190 were identified as Konicacronema protuberans Jusko & Johansen 2024, demonstrating sequence similarities ranging from 98.7% to 99.3% with existing sequences of this species. Phylogenetic analyses further corroborate their placement within the Konicacronema genus, forming a robustly supported evolutionary clade. Moreover, the 16S—23S ITS secondary structure of these strains aligns with that of K. protuberans. Employing a polyphasic approach that integrates molecular, morphological, and ecological data, we confirm that strains APE127, APE132, and APE190 represent K. protuberans. These findings constitute the first records of this genus/species in China, expanding its known geographical distribution.

     

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