无机盐诱导蓝藻细胞液泡化
CELL VACUOLIZATION INDUCED BY INORGANIC SALTS IN CYANOBACTERIUM
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摘要: 选用NaCl、KNO3、(NH4)2SO4、MgSO4、CaCl2五种常用无机盐对分属三科的六种蓝藻进行液泡化诱导,不同蓝藻其盐敏感性不同。鱼腥藻sp.595最为敏感,五种盐均诱导其液泡化;颤藻284和极大螺旋藻438最不敏感,所试验盐类均不能诱导其液泡化;念珠藻sp.96、织线藻246和伪枝藻248液泡化程度居中,少数盐类如NaCl、KNO3、(NH4)2SO4对其有诱导作用。采用压片法观察到诱导形成的液泡,液泡在相差显微镜下显示为圆球形,基本透明。Abstract: Six cyanobaceria were cultured in the presence of NaCl、KNO3、(NH4)2SO4、MgSO4 and CaCl2, respectively for vacuole induction, and produced different effects. Anabaena sp. 595 was the most sensitive, vacuole could be induced by all salts tested. In contrast, no salts could induce cell vacuolization in Oscillatoria animlis 284 and Spirulina maxima 438 . While Nostoc sp. 96、Plectonema boryanum 246 and Scytonema hofmanni 248 were the relatively sensitive strains, only NaCl、KNO3、or(NH4)2SO4 revenaled the inducing effect on them. The induced vacuoles were examined by pressing the sample, and it was spherical and transparent in the scope of phase microscope.
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Keywords:
- Cyanobacterium /
- Inorganic salt /
- Vacuole
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