摘要:
本研究选择当年草鱼为试验对象,放入水族箱内,分为试验组和对照组。每组两个平行样,灭幼脲Ⅲ号(CCU)和除虫脲(DFB)的暴露浓度分别都为5和50μg/L。未放鱼的水族箱作为对照。实验开始后,分别在0,6,24,48,72和120h后取样分析两种母体化合物浓度。CCU和DFB在水中的溶解度,用标准柱提取法测定为0.21mg/L和0.23mg/L(温度19℃±1℃),试验鱼体內类脂含量用1987年OECD法测定为1%(湿重计),水样与鱼样中的DFB与CCU,分别在样品纯化步骤后用高效液相色谱法进行测定。本实验结果表明:无论对照箱还是养鱼箱中,同DFB相比,水中CCU都消失得更快,同时它在鱼体内的积累值较低(BCF<100),说明CCU比DFB具有更强的生物可降解性。两种化学品在鱼体内的积累过程与水中暴露浓度有关。
关键词:
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归趋
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积累
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代谢
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降解
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鱼
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水
Abstract:
Grass carp were exposed to 5 and 50 μg/L of diflubenzuron (DFB) and 1-(4-chlorophenyl)-3-(2-chlorobenoyl) urea (CCU) for 5 days. Water samples were analyzed for the parent compounds after 0, 6, 24, 48 and 72 h. Tanks without fish were used as controls. Water solubilities for DFB and CCU, measured by standard column extraction method, were 0.21 mg/L and 0.23mg/L respectively (Temp. 19℃±1℃). Lipid content of fish, determined by OECD method, 1987, was 1% (wet weight). HPLC was used for residue analysis of water and fish samples after they were purified. The results showed that, compared to DFB, CCU disappeared more rapidly from water both in control and in fish tanks. Meanwhile, its bioaccumulation value in fish was relatively low (BCF5<100), indicating a higher biodegradability in CCU than in DFB. The Bioaccumulation processes for both chemicals were related to their exposure concentration in water.
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