不同水温和给药方式下磺胺甲噁唑在草鱼体内的药动学研究
A STUDY ON PHARMACOKINETIC OF SULPHAMETHOXAZOLE IN GRASS CARP AT DIFFERENT TEMPERATURES AND ADMINISTRATION REGIMES
-
摘要: 在18℃和28℃的不同水温条件下，分别采用口灌和腹腔注射的给药方式，给予草鱼100mg/kg体重单剂量的磺胺甲唑(SMZ)，以HPLC法测定草鱼血浆和肌肉中药物浓度，用MCPKP药代动力学软件处理药时数据，结果表明，18℃条件下，草鱼口灌SMZ的分布半衰期T1/2α、消除半衰期T1/2β、达峰时间Tp均显著长于28℃(P<；0.01)，其峰浓度Cmax显著低于28℃下的值(P<；0.01)；腹腔注射给药时，SMZ在鱼体血浆和肌肉中的吸收与分布较口服快，消除较口服也快。在水温18℃条件下，口灌SMZ在草鱼血浆中的药时数据符合带时滞的二室开放模型，腹腔注射的符合无时滞二室开放模型；水温28℃条件下，口灌SMZ在草鱼血浆中的药时数据符合可忽略时滞的二室开放模型。根据SMZ的药代动力学规律、最低有效血药浓度和抗菌药物应用的一般原则，制定了SMZ的用药方案：治疗温水性鱼类细菌性疾病，在水温18℃左右，口服或注射100mg/kg剂量的SMZ，以每天给药两次，3d一个疗程为宜；在水温28℃左右，以每天给药3-4次为宜。该项研究全面了解了在不同水温和不同给药方式下SMZ在鱼体内的药动学规律，为确定合理的临床用药方案以及无公害水产品中药物残留监测提供了可靠的理论依据。Abstract: At water temperature of 18℃ or 28℃,grass carp was administered orally or by intraperitoneal injection with sulphamethoxazole (SMZ) in single dose of 100mg/kg. The concentration of SMZ in the plasma and muscles of the fish were determined by high performance liquid chromatography and the concentration-time data were treated with MPKP practical pharmacokinetic software. The results showed that the elimination half-time (T 1/2α ),the elimination half-time (T 1/2β ) and the peak time of SMZ at oral administration at lower temperature were remarkably longer than those at higher temperature (P0.01). The peak concentration at lower temperature was significantly lower than that at higher temperature (P0.01). Absorption and elimination of SMZ in the plasma and muscle were quicker when administered by intraperitoneal injection than those when administered orally. and its elimination was quicker by intraperitoneal injection than at oral administration. At the temperature of 18℃,the profile of SMZ in the plasma could be described by a two-compartment open model with lag-time when administered orally. It could be described by a two-compartment model without lag-time when administered by intraperitoneal injection. At the temperature of 28℃,the profile could be described by a two-compartment open model with an illegible lag-time. Based on the pharmacokinetics of SMZ,the minimum effective blood drug concentration and the general principle in application of antibiotics,SMZ administration for grass carp is recommended to be in a 3 days treatment administered orally or by intraperitoneal injection twice a day,at 18℃ or 3-4 times a day at 28℃ at 100mg/kg.
-
Keywords:
- Sulphamethoxazole /
- Grass carp /
- Pharmacokinetic /
- Water temperature /
- Administration regimes
-
-
[1] Editorial board of "A handbook of medicines for aquatic animals"of Ministry of Agriculture.A Handbook of Medicines for Aquatic Animals[M].Beijing;China Science and Technology Publishing House,1998,195-196.[农业部"渔药手册"编撰委员会.渔药手册. 北京:中国科技出版社,1998,195-196]
[2] Ge Y H,Zhou J L. Studies on pharmacokinetics of SMZ and SMZ-TMP in domestic rabbits[J].Journal of Hebei Agricultural University,1989,12(1):123-125.[葛印怀,周建玲.磺胺甲基异(口恶)唑(SMZ)与复方磺胺甲基异(口恶)唑(SMZ-TMP)在家兔体内代谢动力学的研究.河北农业大学学报,1989,12(1):123-125]
[3] Liu C Z,Ai X H.A method for the detection of sulphamethoxazole and trimethoprim content in aquatic products with high performance liquid chromatography[J].Freshwater Fisheries,2004,34(2):23-24.[刘长征,艾晓辉.水产品中磺胺甲(口恶)唑与甲氧苄啶含量的高效液相色谱测定法.淡水渔业,2004,34(2):23-24]
[4] itten-handed crab,Eriocheir sinensis[J]. Acta Hydrobiologica Sinica,2003,27(1):18-22.[杨先乐,刘至治,横山雅仁.盐酸环丙沙星在中华绒鳌蟹体内药物代谢动力学研究.水生生物学报,2003,27(1):18-22. Yang X L,Liu Z Z,Masahito YOKOYAMA.Pharmacokinetics of ciprofloxacinum in Chinese mitten-handed crab,Eriocheir sinensis[J]. Acta Hydrobiologica Sinica,2003,27(1):18-22.[杨先乐,刘至治,横山雅仁.盐酸环丙沙星在中华绒鳌蟹体内药物代谢动力学研究.水生生物学报,2003,27(1):18-22]
[5] Ai X H,Chen Z W,Zhang C W,et al.Studies on pharmacokinetics and tissue concentration of olaquindox in carps[J].Acta Hydrobiologica Sinica,2003,27(3):56-60.[艾晓辉,陈正望,张春光,等. 喹乙醇在鲤体内的药物代谢动力学及组织浓度研究[J].水生生物学报,2003,27(3):56-60]
[6] Fang W H,Shao J H,Shi Z H. Analytical method of norfloxacin in the giant tiger shrimp(Penaeus monodon)hemolymph and brief study on pharmacokinetics[J]. Acta Hydrobiologica Sinica,2003,27(1):13-17.[房文红,邵锦华,施兆鸿,等.斑节对虾血淋巴中诺氟沙星含量测定及药代动力学.水生生物学报,2003,27(1):13-17]
[7] Xia W J,Cheng Z R.MCPKP-A microcomputer programme for kinetic analysis of drugs[J].Journal of Pharmacology of China, 1988,9(2):188-192.[夏文江,成章瑞.MCPKP-药物动力学分析的一种微机程序.中国药理学报,1988,9(2):188-192]
[8] Herman R L,Degurse P E.Sulfamerazine residues in trout tissues[J].Ichthyologica,1967, 39:73-79
[9] Lee M J,Kou G H.Absorption of sulfamonomethoxine by eels in medicated bath [J]. Fish Series,1978,34:69-75
-
期刊类型引用(6)
1. 陈辰，滑少波，李杰勤，姜华，黄鹤平，姜婉琦，姚敏学，李向飞，初晓辉，刘秦华. 紫花苜蓿青贮饲料对草鱼生长性能和肌肉品质的影响. 草地学报. 2024(09): 2990-2998 . 
百度学术
2. 李彤，司梦雅，崔青曼，袁春营. 紫花苜蓿对中华绒螯蟹生长性能、消化酶活性及代谢相关因子基因表达的影响. 饲料研究. 2023(11): 60-65 . 百度学术
3. 陈拥军，邹滔，林仕梅，罗莉，李云. 草鱼体组成的数学描述. 水产学报. 2016(04): 566-576 . 百度学术
4. 宋坚，何舟，程龙，庞云龙，宁军号，常亚青. 饲料添加苜蓿草粉对刺参幼参生长、体成分及免疫酶的影响. 草业学报. 2015(02): 208-214 . 百度学术
5. 宋盛亮，李君华，李静，刘佳亮，曹学彬，曲玺昌，王洪敏. 花生秧部分替代马尾藻对仿刺参生长、消化的影响. 水产科学. 2014(11): 685-691 . 百度学术
6. 李旭，章世元，陈四清，邹安革，高菲，孙慧玲，燕敬平，刘长琳. 四种饲料原料对刺参生长、体成分及消化生理的影响. 饲料工业. 2013(08): 36-40 . 百度学术
其他类型引用(7)
计量
- 文章访问数:
- HTML全文浏览量: 0
- PDF下载量:
- 被引次数: 13
下载: