几种藻类蛋白核的超微结构研究
STUDIES ON ULTRASTRUCTURE OF PYRENOID FROM SEVERAL ALGAE
-
摘要: 应用电镜及免疫电镜技术对莱茵藻、小球藻、条浒苔和紫菜等藻类的叶绿体蛋白核的超微结构及主要组成成分进行了观察和研究.结果显示:不同藻类的蛋白核结构不同,显示了藻类蛋白核的多样性.蛋白核为球形或椭圆形,由蛋白质组成.莱茵藻、小球藻和条浒苔的蛋白核外围被淀粉鞘所包围,而紫菜的蛋白核外围无淀粉鞘而直接被叶绿体的类囊体所包围.淀粉鞘由淀粉组成,淀粉鞘的厚薄与藻体藻龄及增养状态有关系.在蛋白核中央,一般都具有由类囊体形成的孔道,使蛋白核与外界联系,小球藻和条浒苔蛋白核具有1条纵向孔道,而莱茵藻和紫菜为多条孔道.金相免疫技术检测结果显示Rubisco和Rubisco活化酶均在蛋白核及淀粉鞘区域中定位,表明蛋白核具有光合作用功能.Abstract: This paper mainly dealed with the pyrenoid ultrastructure and gold immunolocalization of Ribulose 1,5-bisphosphate carboxylase(Rubisco)and Rubisco activase in chloroplast of several algae: Chlamydomonas reinhardtii, Chlorella spp. 640909, Enteromorpha clathrata that and Porphyra yezoensis. The results showed that there were different ultrastructure of pyrenoids among these different algae. Pyrenoid shaped in ball or ellipse, was composed by protein. The pyrenoid of Chlamydomonas reinhardtii, Chlorella, E. clathrata, was embedded by starch sheath, whereas the pyrenoid of Porphyra yezoensis had no starch sheath, and embedded by the thylakoids of chloroplast. The starch sheath was composed of starch, its side was related with the age and culture conditions of algae. There were channels in the middle of the pyrenoids, with which the pyrenoids connected the thylakoids of chloroplast. There was one portrait channel in the pyrenoid of Chlorella and E. clathrata, while there were several channels in the pyrenoids of Chlamydomonas reinharditt and Porphyra yezoensis. The results of gold immunolocalizatiopn showed the Rubisco and Rubisco activase distributed in the pyrenoid and starch sheath region. It was demonstrated that the pyrenoid had close relation with photosynthesis.
-
Keywords:
- Algae /
- Pyrenoid /
- Ultrastructure /
- Immunogold localization
-
-
[1] Holdsworth R H. The isolation and partial characterization of the pyrenoid protein of Eremosphaera viridis [J]. J. Cell Biol., 1971, 51:499-513[2] Ramazanov Z, Henk C, Mason C, et al. The induction of the CO2 -concentrating mechanism is correlated with the formation of the starch sheath around the pyrenoid of Chlamydomonas reinhardtii [J]. Planta, 1994, 195:210-216[3] Miyamura S, Hori T, Ohya T, et al. Co-localization of chloroplsat DNA and ribulose-1, 5-bisphosphate carboxylase/oxygenase in the so-called pyrenoid of the siphonous green alga Caulerpa lentillifera(Caulerpales, Chlorophyta)[J]. Phycologia, 1996,35(2):156-160[4] 陈雄文. 环境因子对莱茵藻(Chlamydomonas reinhardtii)生长及其胞外碳酸酐酶表达的影响[D]. 南京农业大学硕士论文,1997[5] 张大兵,李立人,缪有刚,等. 小球藻Rubisco的纯化及其在异养向自养转变过程中的含量变化[J]. 植物生理学报,1994,20(4):346-352[6] 唐如航,贾军伟,李立人.烟草Rubisco活化酶的纯化及其特性[J]. 植物生理学报,1997,23(1):89-94[7] Morita E, Kuroiwa H, Huroiwa T, et al. High localization of Ribulose-1, 5-bisphosphate carboxylase/oxygenase in the pyrenoids of Chlamydomanas reihardtii(Chorophyta), as revealed by cryofixation and immunogold electron microscopy [J]. J. Phycol., 1997, 33:68-72[8] 李伟新,朱仲嘉,刘凤贤. 海藻学概论[M]. 上海:上海科技出版社,1982, 173-217[9] Mckay RML, Gibbs SP. Composition and function of pyrenoids: cytochemical and immuno- cytochemical approaches [J]. Can. J. Bot., 1991,69:1040-1052[10] Fisher P,Klein U. Localization of nitrogen-assimilating enzymes in the chloroplast of Chloamydomonas reinhardtii [J]. Plant Physiol., 1998, 88:149-155[11] Osafune T, Yokota A, Ehara T, et al. Immunogold localization of ribulose-1, 5-bisphosphate carboxylase with reference to pyrenoid morphology in chroloplasts of synchronized Euglena gracilis cells [J]. Plant Physiology, 1990, 92:803-808[12] Kerby NW, Evans LV, Pyrenoid protein from the brown alga Pilayella littoralis [J]. Planta, 1981, 151:469-475[13] Satoh H, Okada M, Nakayama K, et al. Purificatron and further characterization of pyrenoid proteins and ribulose-1, 5-bisphosphate carboxylase- oxygenase from the green alga Bryopsis maxima [J]. Plant Cell Physiol., 1984, 25:1205-1214[14] Lacoste-Royal G, Gibbs S P. Immunocytochemical localization of ribulose-1, 5-bisphosphate carboxylasei in the pyrenoid and thylakoid region of the chloroplast of Chlamydomonas reinhardtii [J]. Plant Physiol., 1987, 83:602-606[15] Kuchitsu K, Tsuzuki M, Miyachi S. Characterization of the pyrenoid isolated from unicellular green alga Chlamydomonus reinhardtii: Particulate from of Rubisco protein [J]. Protoplasma, 1988, 144:17-24[16] Villarejo A, Isabel Orus M, Martinez F. Regulation of the CO2-concentratiing mechanism in Chlorella vulgaris UAM 101 by glucose [J]. Physiologia Plantarum, 1997, 99:293-301[17] Aizawa K, Miyachi S. Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria [J]. FEMS Microbiol. Rev., 1986, 39:215-223 Holdsworth R H. The isolation and partial characterization of the pyrenoid protein of Eremosphaera viridis [J]. J. Cell Biol., 1971, 51:499-513[2] Ramazanov Z, Henk C, Mason C, et al. The induction of the CO2 -concentrating mechanism is correlated with the formation of the starch sheath around the pyrenoid of Chlamydomonas reinhardtii [J]. Planta, 1994, 195:210-216[3] Miyamura S, Hori T, Ohya T, et al. Co-localization of chloroplsat DNA and ribulose-1, 5-bisphosphate carboxylase/oxygenase in the so-called pyrenoid of the siphonous green alga Caulerpa lentillifera(Caulerpales, Chlorophyta)[J]. Phycologia, 1996,35(2):156-160[4] 陈雄文. 环境因子对莱茵藻(Chlamydomonas reinhardtii)生长及其胞外碳酸酐酶表达的影响[D]. 南京农业大学硕士论文,1997[5] 张大兵,李立人,缪有刚,等. 小球藻Rubisco的纯化及其在异养向自养转变过程中的含量变化[J]. 植物生理学报,1994,20(4):346-352[6] 唐如航,贾军伟,李立人.烟草Rubisco活化酶的纯化及其特性[J]. 植物生理学报,1997,23(1):89-94[7] Morita E, Kuroiwa H, Huroiwa T, et al. High localization of Ribulose-1, 5-bisphosphate carboxylase/oxygenase in the pyrenoids of Chlamydomanas reihardtii(Chorophyta), as revealed by cryofixation and immunogold electron microscopy [J]. J. Phycol., 1997, 33:68-72[8] 李伟新,朱仲嘉,刘凤贤. 海藻学概论[M]. 上海:上海科技出版社,1982, 173-217[9] Mckay RML, Gibbs SP. Composition and function of pyrenoids: cytochemical and immuno- cytochemical approaches [J]. Can. J. Bot., 1991,69:1040-1052[10] Fisher P,Klein U. Localization of nitrogen-assimilating enzymes in the chloroplast of Chloamydomonas reinhardtii [J]. Plant Physiol., 1998, 88:149-155[11] Osafune T, Yokota A, Ehara T, et al. Immunogold localization of ribulose-1, 5-bisphosphate carboxylase with reference to pyrenoid morphology in chroloplasts of synchronized Euglena gracilis cells [J]. Plant Physiology, 1990, 92:803-808[12] Kerby NW, Evans LV, Pyrenoid protein from the brown alga Pilayella littoralis [J]. Planta, 1981, 151:469-475[13] Satoh H, Okada M, Nakayama K, et al. Purificatron and further characterization of pyrenoid proteins and ribulose-1, 5-bisphosphate carboxylase- oxygenase from the green alga Bryopsis maxima [J]. Plant Cell Physiol., 1984, 25:1205-1214[14] Lacoste-Royal G, Gibbs S P. Immunocytochemical localization of ribulose-1, 5-bisphosphate carboxylasei in the pyrenoid and thylakoid region of the chloroplast of Chlamydomonas reinhardtii [J]. Plant Physiol., 1987, 83:602-606[15] Kuchitsu K, Tsuzuki M, Miyachi S. Characterization of the pyrenoid isolated from unicellular green alga Chlamydomonus reinhardtii: Particulate from of Rubisco protein [J]. Protoplasma, 1988, 144:17-24[16] Villarejo A, Isabel Orus M, Martinez F. Regulation of the CO2-concentratiing mechanism in Chlorella vulgaris UAM 101 by glucose [J]. Physiologia Plantarum, 1997, 99:293-301[17] Aizawa K, Miyachi S. Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria [J]. FEMS Microbiol. Rev., 1986, 39:215-223
计量
- 文章访问数: 1285
- HTML全文浏览量: 1
- PDF下载量: 481
下载: