多甲藻对不同光照强度的反应:色素沉着及暗光适应的可能性
THE RESPONSE OF PERIDINIUM GATUNENSE TO DIFFERENT LIGHT REGIMES: ITS PIGMENTATION AND POSSIBLE IMPLICATIONS ON THE LIGHT-SHADE ADAPTATION
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摘要: 在HPLC色素分析的基础上,研究了多甲藻对不同光照强度(16.6-250μ·Em-2s-1)的反应.类胡萝卡素中最重要的多甲藻素和硅甲藻黄素占叶黄素总量的90%.随光照强度的逐步增加,多甲藻细胞中叶绿素a含量呈现明显的下降趋势.结果表明硅甲藻黄素和β胡萝卜素具有光保护作用:1)当多甲藻暴露于较强光下时,硅甲藻黄素/叶绿素a之比呈现出明显增加的趋势;β胡萝卜素/叶绿素a之比也显现出明显的增加趋势;2)β胡萝卜素/叶绿素a之比在有光条件下比在完全黑暗条件下要高;3)硅甲藻黄素/叶绿素a之比在高辐照度、低辐照度,及由高到低、由低到高辐照度处理时,显示出组间差异.由此可推出色素代谢可能的途径及其湖沼学意义.Abstract: The response of Peridinium gatunense (formerly Peridinium cinctum fa.westii) to different light regimes was investigated based on pigment profiles, which were revealed by HPLC analyses, over the ranges of 16.6-250μE·m-2·S-1 Peridinin and diadinoxanthin are the two most important carotenoids, shared about 90% of the total xanthophylls.Cellular chlorophyll a showed an evident drop along the increased irradiance gradients.Evidence was presented to show the role of diadinoxanthin and β-carotene as light protectants, including: l) diadinoxanthin / chlorophyll a ratios increased drastically when Peridinium was exposed to increased light gradients and β-carotene / chlorophyll a ratios also showed an obvious increasing tendency; 2) β-carotene / chlorophyll a ratios under light condition were higher than those under completely dark condition; and 3) diadinoxanthin / chlorophyll a ratios exhibited inter-group differences among high irradiance, low irradiance, high to low and low to high irradiance treatments.Hereby the possible implication for pigment metabolic routes and their limnological behaviour was deduced.
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Keywords:
- Lake Kinneret /
- Light-shade adaptation /
- Peridinium gatunense /
- Pigmentation
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