力竭性运动后鲇鱼幼鱼乳酸、糖原和葡萄糖水平的变动
LACTATE, GLYCOGEN AND GLUCOSE LEVELS OF JUVENILE CATFISH (SILURUS ASOTUS LINNAEUS) IN RESPONSE TO EXHAUSTIVE EXERCISE
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摘要: 于(25±1)℃的条件下分别测定了鲇鱼(Silurus asotusLinnaeus)幼鱼静止状态(对照组)与力竭性运动后不同恢复时间(0、0.5、1、2、48、16h)肌肉、血液和肝脏三种组织中乳酸、糖原和葡萄糖的含量水平. 结果显示: 鲇鱼幼鱼力竭性运动后肌乳酸和血乳酸水平迅速上升并即刻达到峰值(12.13±0.19)μmol/g和(4.57±0.23)mmol/L, 而肝乳酸的峰值((9.78±0.69)μmol/g)却出现在运动后恢复1h;肌组织乳酸的迅速上升伴随着糖原的快速下降, 葡萄糖含量保持相对稳定;肌乳酸恢复历时为8h, 清除速率为0.9μmol/h.g. 实验结果表明鲇鱼力竭性运动后存在着明显的"乳酸泄露"现象, 其无氧代谢的底物主要是糖原;肝组织可能是一个乳酸的临时贮存库.Abstract: The lactate, glycogen and glucose levels in whitemuscle, blood and liver of juvenile catfish (Silurus asotus Linnaeus) were measured at rest(rest ing controls) and during different recovery periods (0、0. 5、1、2、4、8、16h) after exhaustive exercise at (25±1) e in this study. The results showed that lactate levels in muscle and blood increased to peak value immediately, reaching (12. 13±0. 19) Lmol/g and (4.57±0. 23) mmol/L, respectively. While the liver lactate reached to the maximum(9. 78±0. 69) Lmol/g during recovery at 1h post exercise. The increase in muscle lactate levels after exercise agreed well with the decrease in its glycogen levels, whereas concentrat ion of glucose remained relatively unchanged during this period. Muscle lactate returned to resting levels within 8h of recovery and its lactate clearance rate was 0.9Lmol/h·g. These results of our study suggest that -lactate leaking.-phenomenon actually exists in catfish after exhaustive exercise, glycogen is used as themain fuel source for anaerobic metabolism; the liver may act as a temporary sink for lactate.
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Keywords:
- Silurus asotus Linnaeus /
- Lactate /
- Glycogen /
- Exhaustive exercise /
- Recovery
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