武汉东湖光合色素与叶绿素a代谢产物的HPLC研究
STUDIES ON PHOTOSYNTHETIC PIGMENTS AND CHLOROPHYLL A DERIVATIVES OF LAKE DONGHU BY USING HPLC
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摘要: 用高效液相色谱(HPLC)法研究了武汉东湖周年及围隔实验水柱颗粒物色素的组成及变化.共检测到约20种色素,类胡萝卜素含量较高的有硅藻的标志色素岩藻黄素,隐藻的异黄素,蓝、绿藻的黄体素、玉米黄素及胡萝卜素.东湖叶绿素a的代谢产物主要为脱植基叶绿素a(全湖年均约占叶绿素a的5%),而非脱镁叶绿素a或脱镁叶绿酸a.围隔实验结果表明:叶绿素a与总浮游植物(r=0.84),叶绿素b与绿藻(r=0.77),岩藻黄素与硅藻(r=0.8),异黄素与隐藻生物量(r=0.83)之间具显著的相关性.表明用HPLC分析色素快速、简捷,是研究浮游植物群落组成及动态变化的有力辅助工具.Abstract: Pigments in seston of Lake Donghu and enclosures were studied by means of HPLC. There were totally 20 pigments separated in the study and the major carotenoids were fucoxanthin, alloxanthin, lutein, and zeaxanthin. The annual average concentration of each pigment at Station Ⅰ was always the highest, and Station Ⅱ ranked the second. Chlorophyllide a was the major degradation product of Chl a, comprising about 5% of the Chl a concentration. Other Chl a derivatives appeared mostly in minor quantities. The results of the enclosure experiment showed that there was a significant correlation between the concentration of the marker pigment and the biomass of the correspondent algae group. The correlation coefficients between Chl a and total phytoplankton biomass, Chl b and green algae, alloxanthin and cryptophytes, fucoxanthin and diatoms were 0.84,0.77,0.83and 0.68,respectively. The results suggest that pigment analysis is a useful tool for monitoring phytoplankton structure because it is rapid and time saving.
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