鱼微核试验筛检水体诱变物的应用与研究
THE APPLICATION AND STUDIES OF MICRONUCLEUS ASSAYS WITH FISH TO SCREEN MUTAGEN IN WATER
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摘要: 对五种环境污染物的遗传毒性采用鱼微核试验技术对其进行了筛检,试验结果表明鱼微核试验用作筛检诱变物的遗传毒性是有效的,对监测水体的质量也具有潜在的价值;同时,对国内外学者将各种各样的技术应用到鱼微核试验进行了总结,概述了微核形成的机理;在此基础上,提出应用鱼微核试验筛检水体诱变物时应注意的问题。Abstract: By using micronucleus assays with fish to screen the genotoxicity of five kinds of environmental pollutants, the results showed that they are useful techniques for genotoxicity testing, and potential for in situ monitoring of water quality; In discussion, the author summarized the various techniques used for micronucleus analysis in fish from references and discussed mechanisms for formation of micronuclei in cells.
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Keywords:
- Micronucleus assays /
- Genotoxicity /
- Cytokinesis-blocked method /
- Fish /
- Water /
- Mutagen
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[1] Harshbarge M A, Clark J B. Epizootiology of neoplasms in bony fish of North America [J]. Sci. Total Envion., 1990,94:1-32[2] 张天保.微核试验筛检化学诱变物的价值及方法学上的研究近况[J].环境与健康杂志,1986,3(1):43-46[3] 欧德有,周青山.湘江底泥中重金属铅对鱼类血相的毒性影响[J].湖南师范大学自然科学学报,1986,2(增刊):53-56[4] 贺维顺,王蕊芳.蝌蚪血红细胞微核和核异常监测水质污染的研究[J].动物学研究,1990,11(1):1-6[5] 沈光平,王钦南.微核与染色体畸变的相关性[J].遗传,1985,7(1):15-17[6] Buckey J A. Heinz Body hemolytic anemia in Coho Salmon (Oncorhynchus kisutch) exposed to chlorinated wastewater [J]. J. Fish. Res. Board. Can., 1977, 34: 215-224[7] 郭永灿,周青山.镉对白鲢外周血红细胞微核率影响的观察[J].湖南师范大学自然科学学报,1985,3:79-83[8] 唐明德,易义珍.百菌清诱导鳝鱼血液有核红细胞微核率的研究[J].湖南医学院学报,1987,12(2):153-155[9] Schultz N, Norrgren L, Grawe J A. Micronuclei frequency in circulating erythrocytes rom rainbow trout (Oncorhynchus mykiss) subjected to radiation, an imageanalysis and flow cytometric study [J]. Comp Biochem. Physiol., 1993, 105C:207-211[10] Hose J E, Cross J N, Smith S G, et al. Elevated circulating erythrocyte micronuclei in fishes from contaminated sites off Southern California [J]. Marine Environ. Res., 1987, 22:167-176[11] Williams R C, Metcalfe C D. Development of an invivo hepatic micronucleus assay with rainbow trout [J]. Aquatic Toxicology, 1992, 23:193-202[12] Ueda T M, Hayashi M, Ohtsuka Y. A preliminary study of the micronucleus test by acridin orange fluorescent staining compared with chromosomal aberration test using fish erythropoietic and embryonic cells [J]. Water Sci. Tech., 1992, 25(11):235-240[13] K. Al-Sabti. An in vitro binucleated blocked hepatic cell technique for genotoxicity testing in fish [J]. Mutation Research, 1995, 335:109-120[14] Heddle J A. A rapid in vivo test for chromosomal damage [J]. Mutation Research, 1973, 18:307-317[15] Schmidt W. The micronucleus test [J]. Mutation Research, 1975, 31:9-15[16] 刘爱华,施立明.鱼类血细胞的微核测定[J].动物学研究,1985,6:8-10[17] Kabil Al-sabti, Chris D Metcalfe. Fish micronuclei for assessing genotoxicity in water [J].Mutation Research, 1995, 343:121-135[18] 薛开先,季国芳.微核形成与细胞周期关系的初步研究[J].遗传学报,1986,13(5):397-402 Harshbarge M A, Clark J B. Epizootiology of neoplasms in bony fish of North America [J]. Sci. Total Envion., 1990,94:1-32[2] 张天保.微核试验筛检化学诱变物的价值及方法学上的研究近况[J].环境与健康杂志,1986,3(1):43-46[3] 欧德有,周青山.湘江底泥中重金属铅对鱼类血相的毒性影响[J].湖南师范大学自然科学学报,1986,2(增刊):53-56[4] 贺维顺,王蕊芳.蝌蚪血红细胞微核和核异常监测水质污染的研究[J].动物学研究,1990,11(1):1-6[5] 沈光平,王钦南.微核与染色体畸变的相关性[J].遗传,1985,7(1):15-17[6] Buckey J A. Heinz Body hemolytic anemia in Coho Salmon (Oncorhynchus kisutch) exposed to chlorinated wastewater [J]. J. Fish. Res. Board. Can., 1977, 34: 215-224[7] 郭永灿,周青山.镉对白鲢外周血红细胞微核率影响的观察[J].湖南师范大学自然科学学报,1985,3:79-83[8] 唐明德,易义珍.百菌清诱导鳝鱼血液有核红细胞微核率的研究[J].湖南医学院学报,1987,12(2):153-155[9] Schultz N, Norrgren L, Grawe J A. Micronuclei frequency in circulating erythrocytes rom rainbow trout (Oncorhynchus mykiss) subjected to radiation, an imageanalysis and flow cytometric study [J]. Comp Biochem. Physiol., 1993, 105C:207-211[10] Hose J E, Cross J N, Smith S G, et al. Elevated circulating erythrocyte micronuclei in fishes from contaminated sites off Southern California [J]. Marine Environ. Res., 1987, 22:167-176[11] Williams R C, Metcalfe C D. Development of an invivo hepatic micronucleus assay with rainbow trout [J]. Aquatic Toxicology, 1992, 23:193-202[12] Ueda T M, Hayashi M, Ohtsuka Y. A preliminary study of the micronucleus test by acridin orange fluorescent staining compared with chromosomal aberration test using fish erythropoietic and embryonic cells [J]. Water Sci. Tech., 1992, 25(11):235-240[13] K. Al-Sabti. An in vitro binucleated blocked hepatic cell technique for genotoxicity testing in fish [J]. Mutation Research, 1995, 335:109-120[14] Heddle J A. A rapid in vivo test for chromosomal damage [J]. Mutation Research, 1973, 18:307-317[15] Schmidt W. The micronucleus test [J]. Mutation Research, 1975, 31:9-15[16] 刘爱华,施立明.鱼类血细胞的微核测定[J].动物学研究,1985,6:8-10[17] Kabil Al-sabti, Chris D Metcalfe. Fish micronuclei for assessing genotoxicity in water [J].Mutation Research, 1995, 343:121-135[18] 薛开先,季国芳.微核形成与细胞周期关系的初步研究[J].遗传学报,1986,13(5):397-402
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