Abstract: Streptomyces violaceoruber and Xanthobacter autotrophics had been isolated from soil in the plague spot of Dongting lake by a selective culture medium and cultured about 30 hours in lab.,and its culture solution were diluted by 1000 times and used to kill Oncomelania hupensis, results indicated that effects of Streptomyces violaceoruber culture solution killing Oncomelania hupensis were better than that of Xanthobacter autotrophics culture solution after Oncomelania hupensis were marinated by different time in 5000ml wide-necked bottle,ratio of killing Oncomelania hupensis reached individually 90%,85%;When every kind of microbial pesticides was diluted by 1000 times for killing Oncomelania hupensis, effects of Huaronghao killing Oncomelania hupensis was better than that of BTbiopesticide,Kenuoteshaming biopesticide、Kenuoshudanbiopesticide and Kenuoqianshengbiopesticide,its percentages ratios of killing Oncomelania hupensis reached to 100% after being marinated during 48-72 hours and when all kinds of microbial pesticides were diluted and sprinkled to soil for killing Oncomelania hupensis, results indicated Huaronghao of them is still the best microbial biopesticide,Its percentages ratio of killing Oncomelania hupensis reached 100% as the same as that of pentachlorophen as a comparison diluted by 1000 times,the ratios of killing Oncomelania hupensis of pure water was 0%.