中国红树科7种红树植物遗传多样性分析
ANALYSIS ON GENETIC DIVERSITY OF SEVEN MANGROVES IN RHIZOPHORACEAE IN CHINA
-
摘要: 以红树、红海榄、秋茄、角果木、木榄、海莲、尖瓣海莲等7种红树科植物为材料,采用改进的CTAB法获得了纯度较高、得率高、片段完整的基因组DNA.通过筛选出的15个有效引物进行RAPD分析,探讨了7种红树植物间的亲缘关系.15个有效引物共扩增出617条DNA带,其中多态性条带415条,占总扩增条带的6726%.利用Nei指数法得出7个分类群间的遗传一致度和遗传距离,并运用UPGMA法进行聚类分析.7个分类群分为A、B两个大组,平均遗传距离为041.将得出的7个分类群的DNA分子分类系统图,与传统的分类进行比较,发现结果相符.同时获得一个OPG05-900的差异片段可作为区分海莲和尖瓣海莲的分子标记.Abstract: DNAs from 7 txax of mangroves in Rhizophoraceae were extracted by using the CTAB method. A 260 /A 280 ratio of DNA solutions ranged from 1.6 to 2.0,and the length of DNA fragment was about 23kb. RAPD markers were used to assess the relationships among the 7 taxa of mangroves in Rhizophoraceae. Fifteen effective primers were screened from 30 10-oligonucleotide arbitrary primers,and a total of 617 DNA bands were amplified,among which 415(67.26%)were polymorphic. Based on UPGMA cluster analysis of 617 DNA bands amplified by the 15 primers,a DNA molecular dendrogram was established,which divided 7 species in Rhizophoraceae into 2 main groups. The average genetic distance among the 7 taxa was 0.41,and the maximum genetic distance was 0.56(0.6),which between Ceriops tagal and Bruguiera sexangula var. rhynchopetala. It indicated that the 7 taxa are in the same family. Group A included 3 taxa-Rhizophora apiculata,R.stylosa,Ceriops tagal;group B contained 4 species Bruguiera gymnorrhiza,B. sexangula,B. sexangula var. rhynchopetala,and Kandelia candel. The dendrogram obtained from the cluster analysis was consistent to morphological classification. A special DNA fragment,which named OPG05-900,was amplified to distinguish B. sexangula and B. sexangula var. rhynchopetala. The results could be valuable to the projects of plant introduction, ecological restoration and conservation in mangroves.
-
Keywords:
- Rhizophoraceae /
- RAPD /
- Genetic diversity
-
-
[1] 林鹏.中国红树林生态系[M].北京;科学出版社.1997[2] Yang X,Quiros C,Identification and Classification of Celery Cultivars with RAPD Marker[J] Theor.Appl.Genet.,1993;86:205-212[3] 陈洪,朱立煌,徐吉臣等,水稻RAPD分子连锁图谱的构建[J].植物学报,1995,37;677—684[4] Penner G A,Chong J,Levesque,et al.Identification of a RAPD marker linked to the oat stem rust gene Pg3[J],Theor.Appl.Genet.,1993,85:702—705[5] Nei M,Li W.A mathematical model for studying genetic variation in the terms of restriction endonucleases[J],Proc.Natl Acad Sci.USA,1979,17:5269-5273[6] Behnke H D.Heidelberg sieve-element plastids of Cryillaceae and Rhizophoraceae:description significance of subtype PV plastids[J].Pl Syst Evol.,1982,141:31-39[7] 葛菁萍,杨志伟,林鹏.红树植物木榄种群遗传变异与遗传分化的初步研究[J].海洋学报2000,22(4);90—95 林鹏.中国红树林生态系[M].北京;科学出版社.1997[2] Yang X,Quiros C,Identification and Classification of Celery Cultivars with RAPD Marker[J] Theor.Appl.Genet.,1993;86:205-212[3] 陈洪,朱立煌,徐吉臣等,水稻RAPD分子连锁图谱的构建[J].植物学报,1995,37;677—684[4] Penner G A,Chong J,Levesque,et al.Identification of a RAPD marker linked to the oat stem rust gene Pg3[J],Theor.Appl.Genet.,1993,85:702—705[5] Nei M,Li W.A mathematical model for studying genetic variation in the terms of restriction endonucleases[J],Proc.Natl Acad Sci.USA,1979,17:5269-5273[6] Behnke H D.Heidelberg sieve-element plastids of Cryillaceae and Rhizophoraceae:description significance of subtype PV plastids[J].Pl Syst Evol.,1982,141:31-39[7] 葛菁萍,杨志伟,林鹏.红树植物木榄种群遗传变异与遗传分化的初步研究[J].海洋学报2000,22(4);90—95
计量
- 文章访问数: 808
- HTML全文浏览量: 0
- PDF下载量: 419
下载: