中国红树科7种红树植物遗传多样性分析
ANALYSIS ON GENETIC DIVERSITY OF SEVEN MANGROVES IN RHIZOPHORACEAE IN CHINA
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摘要: 以红树、红海榄、秋茄、角果木、木榄、海莲、尖瓣海莲等7种红树科植物为材料,采用改进的CTAB法获得了纯度较高、得率高、片段完整的基因组DNA.通过筛选出的15个有效引物进行RAPD分析,探讨了7种红树植物间的亲缘关系.15个有效引物共扩增出617条DNA带,其中多态性条带415条,占总扩增条带的6726%.利用Nei指数法得出7个分类群间的遗传一致度和遗传距离,并运用UPGMA法进行聚类分析.7个分类群分为A、B两个大组,平均遗传距离为041.将得出的7个分类群的DNA分子分类系统图,与传统的分类进行比较,发现结果相符.同时获得一个OPG05-900的差异片段可作为区分海莲和尖瓣海莲的分子标记.Abstract: DNAs from 7 txax of mangroves in Rhizophoraceae were extracted by using the CTAB method. A 260 /A 280 ratio of DNA solutions ranged from 1.6 to 2.0,and the length of DNA fragment was about 23kb. RAPD markers were used to assess the relationships among the 7 taxa of mangroves in Rhizophoraceae. Fifteen effective primers were screened from 30 10-oligonucleotide arbitrary primers,and a total of 617 DNA bands were amplified,among which 415(67.26%)were polymorphic. Based on UPGMA cluster analysis of 617 DNA bands amplified by the 15 primers,a DNA molecular dendrogram was established,which divided 7 species in Rhizophoraceae into 2 main groups. The average genetic distance among the 7 taxa was 0.41,and the maximum genetic distance was 0.56(0.6),which between Ceriops tagal and Bruguiera sexangula var. rhynchopetala. It indicated that the 7 taxa are in the same family. Group A included 3 taxa-Rhizophora apiculata,R.stylosa,Ceriops tagal;group B contained 4 species Bruguiera gymnorrhiza,B. sexangula,B. sexangula var. rhynchopetala,and Kandelia candel. The dendrogram obtained from the cluster analysis was consistent to morphological classification. A special DNA fragment,which named OPG05-900,was amplified to distinguish B. sexangula and B. sexangula var. rhynchopetala. The results could be valuable to the projects of plant introduction, ecological restoration and conservation in mangroves.
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Keywords:
- Rhizophoraceae /
- RAPD /
- Genetic diversity
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