温度和食物浓度对萼花臂尾轮虫休眠卵形成的影响
EFFECT OF TEMPERATURE AND FOOD CONCENTRATION ON RESTING EGG FORMATION OF FRESHWATER ROTIFER BRACHIONUS CALYCIFLORUS
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摘要: 应用群体累积培养法,在3种温度(20℃、25℃和30℃)和3种斜生栅藻浓度(6.0×106cells/mL、3.0×106cells/mL和1.5×106cells/mL)共9个条件组合下对萼花臂尾轮虫休眠卵形成的研究表明,温度为20℃和食物浓度为6.0×106cells/mL的条件组合下轮虫休眠卵的产量和形成效率最大,此为轮虫休眠卵规模化生产的最适条件;6.0×106cells/mL和3.0×106cells/mL的食物浓度分别是25℃和30℃下轮虫休眠卵生产的最适食物浓度.20℃时轮虫的混交雌体百分率显著大于25℃和30℃时,而温度和食物浓度以及两者间的交互作用均对混交雌体受精率无显著的影响.Abstract: The effect of temperature and food concentration on resting egg formation of freshwater rotifer Brachionus calyciflorus was studied by accumulative population culture under 9 treatments of 3 temperature gradients (20℃, 25℃ and 30℃) and 3 food concentration gradients (1.5 ×106cells / mL,3.0 ×106cells / mL and 6.0 ×106cells /mL). The results showed that under the culture condition of temperature 20℃ and food concentration 6.0×106cells / mL, the production and formation efficiency of resting egg were 2032.33±141.50 ind./(10mL. 13d) and 2.61±0.18 ind./106cells algae, respectively, and were both biggest among all the conditions. This condition was optimal for mass production of resting eggs. At temperature 25℃ and 30℃, the food concentration of 6.0 ×106cells / mL and 3.0 ×106cells / mL was also optimal for the production of resting eggs, respectively. At the temperature 20℃, the mixis rate was bigger than that of 25℃ and 30℃. There was no significant effect of temperature,food concentration and their interaction on the fertilization rate.
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Keywords:
- Brachionus calyciflours /
- Resting egg /
- Production /
- Formation efficiency /
- Temperature /
- Food concentration
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