圆形碘泡虫孢子发生的超微结构研究
STUDIES ON THE ULTRASTRUCTURE OF THE SPOROGENESIS OF MYXOBOLUS ROTUNDUS NEMECZCK
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摘要: 寄生于鲫的圆形碘泡虫的孢子发生过程中,最早可认识阶段的营养体是一个单核原初细胞,原初细胞通过分裂直接在细胞内产生生殖细胞,形成一个细胞包围另一个细胞的状态,在以后的过程中,包围细胞不再分裂,生殖细胞进行一系列的分裂,形成双孢子型泛孢子母细胞.生殖细胞分化成10个细胞,形成二个产孢子单元,每个产孢子单元由5个细胞组成,两个壳瓣原细胞位于两边包围着两个极囊原细胞和一个双核孢子质细胞,最后形成两个孢子.Abstract: This paper dealt with the sporogensis of Myxobolus rotundus Nemeczek, 1911 from Carassius auratus. Sporogensis of M. rotundus was observed by transmission electron microscopy. The earliest stage of the parasite was a primary cell which could give rise to generative cells by fission. Sporogensis was initiated by the envelopment of one generative cell with a enveloping cell. The enveloping cell did not divide anymore, whereas the large, generative cell divided to form a disporous pansporoblast containing 10 cells. Subsequently, the 10 cells began to differentiate and finally formed two 5 cell spore producing units. Each unit consisted of 2 capsulogenic cells and 1 binucleate sporoplasm, surrounded by 2 valvogenic cells.Each capsular primordium and its connecting external tubule formed a polar capsule which contained 7 helically coiled polar filament. The opening of the polar capsule was plugged by a stopper facing the discharge canal. Two valvogenic cells formed the spore valves. A desmosome like junction appeared in close contact the suture between two valvogenic cells. There were numerous microtubules at both sides of sutural ridge. The binucleated sporoplasm cell contained numerous sporoplasmosomes and spherical lipid inclusions in different size. Iodinophilous vacuoles, membrane bounded granules and membrane bounded vacuoles existed in the sporoplasm cell.In nearly mature spores, there was an unclear zones among sporoplasm cells. Several finger like extensions of sporoplasm extended to the zones.Other ultrastructural features were the same as those found in other myxosporeans, including absence of centrioles, the structure of mitochondria, distribution patterns of ribosomes and existence of glycogen particles, etc.
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Keywords:
- Carassius auratus /
- Myxobolus rotundus /
- Sporogensis
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ultrastructure of the plasmodium wall and sporogenesis [J].J.Protozool, 1979b, 26, 574—583[3] Yamamoto T, Sanders J E.Light and electron microscopic observations of sporogenesis in the Myxosporida, Ceratomyxa shasta(Noble, 1950)[J].J.Fish.Dis, 1979, 2, 411—428[4] Lom J, et al.Fine structure of Sphaerospora renicola Dykova and Lom, 1982 a Myxosporean from carp kidney and comments of the origin of pansporoblasts [J].Protistologica, 1982, 18, 489—502[5] Stehr C.Sporogenesis of the myxosporean Kudoa paniformis Kabata Whitaker, 1981 infecting the muscle of the Pacific whiting, Merluccius productus(Ayres)[J].J.Fish.Dis, 1986, 9, 493—504[6] 曾美棣、邹为民等.饼形碘泡虫在草鱼苗体内发育各阶段的显微及超微结构[J].水生生物学报,1988,12(2):133—136[7] 吴宝华.鲢疯狂病病原体鲢碘泡虫孢子形成各阶段的观察研究[J].动物学报,1993,39,1—3[8] Xiao W H, et al.Ultrastructural observations on sporogenesis of Myxobolus kwangsiensis Hsieh, GongXiao from the kidney of Dace Labeo rohita [J].Chin.J.Oceanol.Limmol, 1996, 14(3):215—221[9] Pulsford A, Matthews R A.Ultrastructural study of Myxobolus exiguus Thelohan, 1895(Myxosporea)from grey mullet, Crenimugil labrosus(Risso)[J].J.Fish.Dis, 1982, 5:509—526[10] Lom J, Dykova I.Comments on myxosporean life cycles [J].Symposia Biological Hungarica, 1986a, 33, 309—318[11] Kent M L, et al.The demise of a class of protists: Taxonomic and nomenclatural revisions proposed for the protists phylum Myxozoa Grasse, 1970 [J].Canadian Journal of Zoology, 1994, 72, 932—937[12] Siddal M E, et al.The demise of a phylum of protists: phylogeny of Myxozoa and other parasitic cnidaria [J].J.Parasitol, 1995, 81(6):961—967[13] 柴建原等.吸鱼粘体虫在异育银鲫心脏中的孢子发生[J].动物学报,1991,37(4):397—401[14] Lom J, Dykova I.Sporogenesis and spore structure in Kudoa lunata(Myxosporea, Multivalvulida)[J].Parasitol Res, 1988, 74, 521—530[15] Lom J.Notes on the ultrastructure and sporoblast development in fish parasitizing myxosporidian of the genus sphaeromyxa [J].Zeitschrift fur Zellforschung, 1969b, 97, 416—437[16] Schubert G.Elektronenmikrosckopische Untersuch ungen zur Sporenentwicklung von Henneguya pinnae Schuber(Sporozoa, Myxosporridia, Myxobolidae)[J].Z.Parasitenk, 1968, 30, 57—77[17] Current W L, Janovy J J.Comparative study of ultrastucture of interlamellar and intralamellar types of Henneguya exilis Kudo from Channel Catfish [J].J.Protozool, 1978, 25(1):56—65[18] Lom J.Notes on the extrusion and some other feature of myxosporidian spores [J].Acta Protozool, 1964, 2, 321—327[19] Walliker D.The nature of the iodinophilous vacuole of Myxosporidan spores, and a proposal to synonymize the genus Myxosoma Thelohan, 1892 with the genus Myxobolus Butschli, 1882 [J].J.Protozool, 1968, 15(3):571—575
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