铜绿微囊藻生物钟蛋白KaiA的自身相互作用研究
SELF-INTERACTION OF CIRCADIAN CLOCK PROTEIN KAIA OF MICROCYSTIS AERUGINOSA IN YEAST TWO HYBRIDIZATION SYSTEM
-
摘要: 正蓝藻虽为原核生物,但它也和真核生物一样具有生物钟,它的固氮作用、光合作用、氨基酸吸收、细胞分裂以及基因表达等生理代谢过程都受到生物钟的调控,具有昼夜节律性。虽然蓝藻生物钟和真核生物钟一样,都以近24h的周期运行,都具有温度补偿效应,光、温等环境因素都能重置生物钟的时相,但组成蓝藻生物钟的钟蛋白与真核生物钟蛋白间不具有任何同源性,蓝藻生物钟的计时机制也与真核生物钟存在差异[1-2]。蓝藻钟基因为一个基因簇kai,由三个基因kaiA、kaiB、kaiC以单一拷贝成簇排列,Kai蛋白组成蓝藻生物钟的核心即中央振荡器,其中kaiC蛋白的磷酸化状态是中央振荡器产生周期性震荡的关键,它决定中央振荡器的时相,而kaiC的磷酸化状态则受到kaiA和kaiB的调节。kaiA是接受和整合环境信息的钟蛋白,具有N-端和C-端两个结构域,N-端缺乏保守天冬氨酰残基的伪接受域能通过与输入途径的某种蛋白(目前未知)发生相互作用而感受环境信号
-
Keywords:
- Microcystis aeruginosa /
- Clock protein /
- Yeast two hybridization
-
-
[1] Johnso C H, Golden S S. Circadian programs in cyenobacteria: adaptiveness and mechanism[J].Annu. Rev. Microbiol. 1999,53:389-409
[2] Ditty J L, Williams S B, Golden S S. Cyenobacteriel circadian time mechanism[J].Annv. Rev. Genet, 2003, 37: 513-543
[3] Shiura M, Kutsuna S, Aoki S.et al. Expression of a clock gene cluster kaiABC as a circadian feedback process in cyanobacteria[J].Science,1998,281:1519-1523
[4] Nishiwaki T, Iwasaki H, lshiura M, et al. Nucleotide binding and autophosphorylation of clock protein KaiC as a circadian timing process of cyanobacteria. PNAS.2000,97:495-499
[5] Taniguchi Y, Yamaguchi A，Iwasaki H, et al.Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. FEBS letters，2001,496:86-90
[6] Williame S B. Vakonakis I. Golden S S. Structure and function from the circadian clock protein KaiA of Synechococcua elongatra:A potential clock input mechanism. PNAS.2002,99(24):15357-15363
[7] Vakonakie I,Sun J,Wn T,Honzenburg A.Golden SS and Liweng AC. NMR sweture of the KaiC-interacting C-terminal domain of KaiA, a circadian clock protein: Implications for KaiA-KaiC interaction. PNAS,2004,101(6):1479-1484
[8] Iwasaki H, Niehiweki T, Kitayama Y, et al.KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria. PNAS. 2002,99(24):15788-15793
[9] Kitayama Y. Iwasaki H.Nishiwaki T, et al.KeiB functions as an attenuator of KaiC phosphorylation in the cyanobecteriel ciecadian clock system. EMBO J. 2003. 22(9): 2127-2134
[10] Smoker J A,Bamum S R. Rapid small-scale DNA isolation from filamentous cyanobecterie. FEMS Microbiol. Letters.1998.56:119-122
[11] Iwasaki H, Taniguchi Y,Ishiura M, et al.Physical interactions among circtufian clock protein KaiA, KaiB and KaiC in cyanobacteria. EMB0. J.1999.18(5):1137-1145
计量
- 文章访问数: 1050
- HTML全文浏览量: 0
- PDF下载量: 453
下载: