双酚A和壬基酚对隆线溞和微型裸腹溞的毒性
THE TOXIC EFFECTS OF BPA AND NP ON D. CARINATA AND M. MICRURA
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摘要: 我们以隆线溞和微型裸腹溞为实验动物,进行了两种酚类内分泌干扰物双酚A(BPA)和壬基酚(NP)的毒性效应研究。急性实验测定出双酚A对隆线溞的24h和48h半数致死浓度(LC50)为:12.02mg/L和11.64mg/L,对微型裸腹溞24h和48h半数致死浓度LC50为:13.70mg/L和9.63mg/L。而壬基酚对隆线溞的24h和48h半数致死浓度(LC50)分别为:0.221mg/L和0.159mg/L,对微型裸腹溞的24h和48h半数致死浓度(LC50)分别为:0.334mg/L和0.126mg/L。实验表明微型裸腹溞对双酚A和壬基酚的敏感度高于隆线溞,能够很好的指示水环境的污染。慢性毒性实验发现双酚A对微型裸腹溞后代的雌雄比例有明显的影响,影响类似于hormesis现象。而壬基酚对隆线溞的慢性毒性研究发现,隆线溞的生活史、后代成活率均受到暴露的壬基酚浓度升高的不利影响庑┭芯勘砻?低浓度双酚A长期暴露的潜在毒性与高浓度壬基酚的急性毒性在这两种内分泌干扰物污染环境的研究中有重要意义。Abstract: In our experiments, Daphnia carinata and Moina micura were used as material to evaluate the toxicity of BPA and NP. From acute experiments, the 24h and 48h LC50 of D. carinata to BPA were 12.02mg/L and 11.64mg/L, the 24h and 48h LC50 of M. micura to BPA were 13.70mg/L and 9.63mg/L, respectively. The 24h and 48h LC50 of D. carinata to NP were 0.221mg/L and 0.159mg/L, the 24h and 48h LC50 of M. micura to NP were 0.334mg/L and 0.126mg/L, re-spectively. It indicated that M. micura was more sensitive to the toxicity of BPA and NP than D. carinata, which offers a good tool to evaluate the pollution of water environment. In chronic experiments, there is a significant relationship between the ratio of male offspring in total offspring and the exposed concentration of BPA and the effects looks like a hormesis phe-nomenon. The life history and the survive rate of offspring exhibited a negative correlation to the exposed concentration of NP, our results suggested that the chronic toxicity of BPA and the acute toxicity of NP were very important to evaluate the pollution of water environment by the two compounds.
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[1] Staples C A,Dorn P D,Klecka G M,et al.A review of the environmental fate,effects,and exposure of bisphenol A[J].Chemosphere,1998,40:521-525
[2] European Union,Risk assessment of 2,2-bis(4-hydroxyphenol) propane (Bisphenol A):Human Health Effects.Document prepared by the UK health and Safety Executive on behalf of the European Union under the existing substances regulation (Council Regulation EEC/793/93).Draft report,July,2001
[3] European Commission,Health & Consumer Protect Directorate General.Opinion of the Scientific Committee on Food on bisphenol A.Brussel Belgium.2002
[4] Baldwin W S,Graham S E,Shea D,et al.Metabolic androgenization of female Daphnia magna by the Xenoestrogen 4-nonylphenol[J].Environmental Toxicology and Chemistry,1997,16:1905-1911
[5] Hense BA,Juttner I,Welzl G,et al.Effects of 4-nonylphenol on phytoplankton and periphyton in aquatic microcosms[J].Environmental Toxicology and Chemistry,2003,22:2727-2732
[6] Hoss S,Juttner I,Traunspurger W,et al.Enhanced growth and reproduction of Caenorhabditis elegans (Nematoda) in the presence of 4-Nonylphenol[J].Environmental Pollution,2002,120:169-172
[7] Jobling S,Sheahan D,Osborne J A,et al.Inhibition of testicular growth in rainbow trout (Oncorhynchus mykiss) exposed to estrogenic alkylphenolic chemicals[J].Environmental Toxicology and Chemistry,1996,15:194-202
[8] Schmude K L,Liber K,Corry T D,et al.Effects of 4-nonylphenol on benthic maeroinvertebrates and insect emergence in littoral enclosures[J].Environmental Toxicology and Chemistry,1999,15:386-393
[9] Huls A G.Acute and chronic toxicity of nonylphenol to Daphnia magna.Final Study Report Numbers DK-522,DL-143,Marl,1992,Germany
[10] Brooke L T.Acute and chronic toxicity of nonylphenol to ten species of aquatic organisms.Report to the US Environmental Protection Agency,Duluth,MN (contract No.68-C1-0034).Lake Superior institute,University of Wisconsin-Superior,Superior,WI.1993
[11] Comber M.H.I,Williams T D,Stewart K M.The effects of nonylphenol on Daphnia magna[J].Water Research,1993,27:273-276
[12] Zhang L,Gibble R,Baer K N.The effects of 4-nonylphenol and ethanol on acute toxicity,embryo development,and reproduction in Daphnia magna[J].Ecotoxicology and Environmental Safety,2003,55:330-337
[13] Liang Y L,Zhang G X.The intrinsic rate of Daphnia carinata King[J].Acta Hydrobiologia Sinica,1964,5:31-35[梁彦龄,张国馨.隆线潘的内桌增长能力.水生生物学集刊.1964,5:31-35]
[14] Jiang X Z and Du N S.Fauna Sinica Crustacea Freshwater Ctadocera.Bejing:Science Press.1979[蒋燮治,堵南山.中国动物志节肢动物门甲壳纲淡水枝角类.北京:科学出版社.1979]
[15] Shi X B.Daphnio carinata and its cultivation[J].Bulletin of Biology,2000,35:14-17[史新柏.隆线潘及其培养.生物学通报,2000,35:14-17]
[16] Majdic G,Sharpo R M,Oshaughnessy P J,et al.Expression of cytochrome P450 17 alpha-hydroxylase/C17-20 lyase in the fetal rat testis is reduced by maternal exposure to exogenous estrogens[J]. Endocrinology,1996,137:1063-1070
[17] Murono E P,Derk R C,de Leon J H.Biphasic effects of octylphenol on testosterone biosynthesis by cultured Leydig cells from neonatal rats[J].Reproductive Toxicology,1999,13:451-462
[18] Alexander H C,Dill D C,Smith L W,et al.Bisphenol A:acute aquatic toxicity[J].Environmental Toxicology,1985,7:19-26
[19] Calabrese E.Evidence that hormesis represents an "overeompensation" response to a disruption in hormeostasis[J]. Ecotoxicology and Environmental Safety, 1999,42:135-137
[20] Stebbling ARD.A theory for growth hormesis[J].Belle News Letter,1997,6(1)
[21] Calabrese E.Estrogen and related compounds:biphasic dose responses[J].Toxicology,2001,31:503-515
[22] Yang H,Zhang K R,Wu D S.The research of BPA detoxifation catalyze by light[J].Chinese Journal of Health Laboratory Technology,2002,12:521-522[杨红,张克荣,吴德生.双酚A光催化降解研究.中国卫生检验杂志,2002,12:521-522]
[23] Gibble R,Bacr K N.Effects of 4-Nonylphenoi on sexual maturation in Daphnia magna[J].Environmental Contamination and Toxicology,2003,70:315-321
[24] Li Z Y.The pollution of BPA and NP in Shihwa Lake[J].Journal of Ocean University of Qingdao,2003,33:847-853[李正炎.西瓦湖中壬基酚和双酚A的污染特征.青岛海洋大学学报,2003,33:847-853]
[25] LeBlane G A,Rider C,Kast-Hutcheson K,et al.Development toxicity of testosterone and chemicals that inhibit steroid clearance in Daphnids:the elusive mechanism.In:SETAC 20《'th》 Annual Meeting,Philaldelphia,PA,November 14-18.Abstract.1999
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