利用鱼肝中视黄化合物和α-生育酚指示水污染毒性的探讨
STUDIES ON RETINIODS AND α-TOCOPHEROL IN LIVER OF SILVER CARP(Hypophthalmichthys molitrix)FROM YAN-JIA LAKE-HIGH ORANOCHLORINE POLLUTED LAKE
-
摘要: 利用高效反相液相色谱法检测了夏冬两季受有机氯污染的典型湖泊──鸭儿湖严家湖区鲢肝中的视黄醇,软脂酸视黄酯及α-生育酚的含量.结果表明,两种营养素在鱼体内的代谢转换明显受环境温度的影响,夏季外界温度较高,肝脏内维生素主要以其生理活性形式存在,表现出视黄醇及α-生育酚含量较高;而冬季外界温度较低,肝脏内维生素主要以其储存形式存在,表现出软脂酸视黄酯含量高.鱼肝中视黄化合物的浓度随氧化塘中污染物浓度的增加而呈现出有规律下降的显着剂量/效应关系,表现在有机氯污染水域中鲢肝中的视黄化合物可作为一种生物标志物指示其污染毒性.Abstract: Retinol, retinyl palmitate and a-tocopherol were measured by reverse-phase HPLC in liver of silver carp(Hypophthalmichthys molitrix)from Yanjia Lake, high organochlorine polluted lake, in summer and winter, 1999. The results indicated that the biotransformation/metabolism of two nutriments were affected by ambient' temperature. In summer while the temperature was higher, vitamins were mainly in their active forms, hence retinol and tocopherol levels in livers of silver carp were higher. But in winter while the temperature was lower, they were mostly in deposited forms, so retinyl palmitat was at higher level than that in summer. Furthermore, increasing trends of retinoids was significantly found in those Samples from pond 1 to pond 5, showing a good dose-response relationship between water contamination and their toxic effects. It is considered that retiniods in fish liver might be used as an effective biomarker in assessing toxic effects of organochlorine pollution in waters.
-
Keywords:
- Retinoids /
- α-Tocopherol /
- HPLC /
- Organochlorine contamination
-
-
[1] Vince P P,Scott B B.HPLC determination of Tocopherol,retinol,dehydroretinol and retinyl palmitate in tissues of lake char(Salvelinus namaycush)exposed to coplanar 3,3',4,4',5-pentachlorobiphenyl[J].Environ.Toxicol.Chem.1994,13:473—476[2] Harry T B.,Esther E F,Martin L E,et al.Alteration in plasma and hepatic retinoid levels in flounder(platichthys flesus)after chronic exposure to contaminated harbour sludge in a mesocosm study[J].Aquat.Toxico,1981,42,271—285[3] Stohs S J,Shara M A,Alsharif N z,et al.2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in female rats.[J].Toxicol.Appl.Pharmacol.,1990,106:126—135[4] Helen H.The role of retinoids in dioxin toxicity.Dioxin'97,Indianapolis,Indiana,USA.1997,34:402—405[5] Hakansson H,Manzoor E Ahlborg U G.Interaction between vitamin A and single oral doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on the TCDD-induced toxity and on the vitamin A status in the rat [J] Nutr.Sci.Vitaminol.1991,37:239—255[6] Arnaud J.,Fortis I.S.Blachier,et al.Simultaneous determination of retinol,α-tocopherol and β-carotene inserum by isocratic high-performance liquid chromatography.[J].J.Chromatogr.1991,572:103—116[7] Spear P A,Bilodeau A Y,Branchard A.Retinoids:From metabolism to environmental monitoring[J].Chemosphere,1992,25:1733—1738[8] Xu Y,Zhang Y Y,Oxynos K.et al.Hexachlorocyclohexane(HCH)residues in Ya-Er lake area,China[J] International Journal of Environmental Analytical Chemistry,1994,57:53—61[9] Wu W Z,Schramm K.-W,Xu Y,et al.PCDD/Fs,PCBs,HCHs and HCB in Sediment and soils of Ya-Er Lake Area in China:Results on Residual levels and correlation to the organic carbon and particle size [J],Chemoshpere,1997,34:191—202[10] Chen G S,Schramm K.-W,Xu Y,et al.Polycyclic Aromatic Hydrocarbon in Ya-Er Lake(Hubei,China):Source and Distribution [J].Fresenius Journal of Analytical Chemistry,1997,359:280—284[11] Chen G S,Xu Y,Xu L H,et al.Influence of dioxin and metal-contaminated sediment on phase I and II biotransformation enzymes in silver crucian carp [J].Ecotoxicology and Environmental safety,1998,40:234—238[12] 徐 盈,吴文忠,张银华,等.湖北鸭儿湖地区六氯环己烷的残留动态与长期归宿研究,水生生物学报,1999,23(4):337—345 Vince P P,Scott B B.HPLC determination of Tocopherol,retinol,dehydroretinol and retinyl palmitate in tissues of lake char(Salvelinus namaycush)exposed to coplanar 3,3',4,4',5-pentachlorobiphenyl[J].Environ.Toxicol.Chem.1994,13:473—476[2] Harry T B.,Esther E F,Martin L E,et al.Alteration in plasma and hepatic retinoid levels in flounder(platichthys flesus)after chronic exposure to contaminated harbour sludge in a mesocosm study[J].Aquat.Toxico,1981,42,271—285[3] Stohs S J,Shara M A,Alsharif N z,et al.2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in female rats.[J].Toxicol.Appl.Pharmacol.,1990,106:126—135[4] Helen H.The role of retinoids in dioxin toxicity.Dioxin'97,Indianapolis,Indiana,USA.1997,34:402—405[5] Hakansson H,Manzoor E Ahlborg U G.Interaction between vitamin A and single oral doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on the TCDD-induced toxity and on the vitamin A status in the rat [J] Nutr.Sci.Vitaminol.1991,37:239—255[6] Arnaud J.,Fortis I.S.Blachier,et al.Simultaneous determination of retinol,α-tocopherol and β-carotene inserum by isocratic high-performance liquid chromatography.[J].J.Chromatogr.1991,572:103—116[7] Spear P A,Bilodeau A Y,Branchard A.Retinoids:From metabolism to environmental monitoring[J].Chemosphere,1992,25:1733—1738[8] Xu Y,Zhang Y Y,Oxynos K.et al.Hexachlorocyclohexane(HCH)residues in Ya-Er lake area,China[J] International Journal of Environmental Analytical Chemistry,1994,57:53—61[9] Wu W Z,Schramm K.-W,Xu Y,et al.PCDD/Fs,PCBs,HCHs and HCB in Sediment and soils of Ya-Er Lake Area in China:Results on Residual levels and correlation to the organic carbon and particle size [J],Chemoshpere,1997,34:191—202[10] Chen G S,Schramm K.-W,Xu Y,et al.Polycyclic Aromatic Hydrocarbon in Ya-Er Lake(Hubei,China):Source and Distribution [J].Fresenius Journal of Analytical Chemistry,1997,359:280—284[11] Chen G S,Xu Y,Xu L H,et al.Influence of dioxin and metal-contaminated sediment on phase I and II biotransformation enzymes in silver crucian carp [J].Ecotoxicology and Environmental safety,1998,40:234—238[12] 徐 盈,吴文忠,张银华,等.湖北鸭儿湖地区六氯环己烷的残留动态与长期归宿研究,水生生物学报,1999,23(4):337—345
计量
- 文章访问数: 862
- HTML全文浏览量: 0
- PDF下载量: 413
下载: