Abstract: Retinol, retinyl palmitate and a-tocopherol were measured by reverse-phase HPLC in liver of silver carp(Hypophthalmichthys molitrix)from Yanjia Lake, high organochlorine polluted lake, in summer and winter, 1999. The results indicated that the biotransformation/metabolism of two nutriments were affected by ambient' temperature. In summer while the temperature was higher, vitamins were mainly in their active forms, hence retinol and tocopherol levels in livers of silver carp were higher. But in winter while the temperature was lower, they were mostly in deposited forms, so retinyl palmitat was at higher level than that in summer. Furthermore, increasing trends of retinoids was significantly found in those Samples from pond 1 to pond 5, showing a good dose-response relationship between water contamination and their toxic effects. It is considered that retiniods in fish liver might be used as an effective biomarker in assessing toxic effects of organochlorine pollution in waters.