中华绒螯蟹受精卵、流产卵氨基酸及脂肪酸组成的比较研究
A COMPARATIVE STUDY ON THE COMPOSITION OF AMINO ACID AND FATTY ACIDIN FERTILIZED EGGS AND LOST EGGS OF ERIOCHEIR SINENSIS
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摘要: 采用气相色谱仪和氨基酸分析仪测定中华绒螯蟹不同种群(太湖种群和温州种群)受精卵和温州种群流产卵脂肪酸及氨基酸的组成.结果表明,中华绒螯蟹太湖种群受精卵、温州种群受精卵和温州种群流产卵在必需氨基酸和半必需氨基酸的含量上无显著差异,而谷氨酸(Glu)、胱氨酸(Cys)和丝氨酸(Ser)三种非必需氨基酸的含量差异显著.太湖种群受精卵、温州种群受精卵及温州种群流产卵的脂肪酸种类分别为16、13和14种.饱和脂肪酸(SFA)占脂肪酸总量的18.96%-23.09%,单烯酸(MUFA)占51.6%-62.65%,多不饱和脂肪酸(PUFA)占14.2%-28.96%,其中最主要的脂肪酸为C16:0、C16:1和C18:1.除C20:1外,其他脂肪酸含量在三者之间都有显著差异.太湖种群受精卵的C18:2、C18:3显著高于温州种群受精卵,而温州种群流产卵的DHA要显著高于太湖种群受精卵.温州种群受精卵和流产卵在C16以下的脂肪酸,其含量均无显著差异.此外,本文还讨论了脂肪酸及氨基酸含量与流产的关系及脂肪酸组成对出苗率的影响.Abstract: The composition of amino acid and fatty acid in fertilized eggs and lost eggs of Eriocheir sinensis were determined with capillary gas chromatography and HPLC.The results showed that there was no difference in the contents of essential amino acid (EAA) and supplemental amino acid (SAA) among the fertilized eggs of Taihu population and Wenzhou population,and the lost eggs of Wenzhou population of Eriocheir sinensis,and the non-essential acid (NEAA),including Glu,Cys and Ser had some difference between the eggs of three groups. There were 16,13 and 14 kinds of fatty acids in fertilized eggs of Taihu population and Wenzhou population,and the lost eggs of Wenzhou population,respectively.Saturated,monounsaturated and polyunsaturated fatty acids represented 18.96%-23.09%,51.76%-62.65%and 14.27%-28.96% of total fatty acids in the eggs respectively.The major fatty acid in each group were C16∶0(14.97%-18.59%),C16∶1(12.07%-20.28%)and C18∶1(37.98%-41.21%).There was significant difference in the contents of fatty acid between the eggs of three groups. The C18∶2,C18∶3 of fertilized eggs of Taihu population were more than that of Wenzhou population,and the DHA of fertilized eggs of Taihu population was much less than that of lost eggs of Wenzhou population.There were no significant changes of C14∶n and C16∶n between the fertilized eggs and the lost eggs of Wenzhou population.The relation between the abortion and the contents of amion acid and fatty acid,and the contents of fatty acid which influence the survival rates were also discussed in this paper.
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Keywords:
- Eriocheir sinensis /
- Amion acid /
- Fatty acid /
- Fertilized eggs /
- Lost eggs
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sinensis)in hot weather[J].Acta Hydrobiologica Sinica,2000, 24 (6):663-665.[朱钧,陈薇,张道南等.高温季节中华绒螯蟹的人工育苗.水生生物学报,2000,24 (6):663-665][7] Zang W L,Jiang M,Dai X L,et al.Effects of Mg2+,Ca2+and Mg2+/Ca2+contents on survival rates of Eriocheir sinensis larvar reared in mixed water[J].Journal of Fisheries of China,1998,22(2):111-115.[臧维玲,江敏,戴习林等.中华绒螯蟹育苗用水中Mg2+与Ca2+含量及Mg2+/Ca2+对出苗率的影响.水产学报,1998,22(2):111-115][8] Zhao Y L,Du N S,Lai W.Effects of different gradient temperature on embryonic development of the Chinese mitten-handed crab,Eriocheir sinensis[J].Zoological Research,1993,14(1):49-53.[赵云龙,堵南山,赖伟.不同水温对中华绒螯蟹胚胎发育的影响.动物学研究,1993,14(1):49-53][9] Talbot P,Zao P.Secretion at molting by the pleopod tegumental glands of the lobster Homarus americanus(Milne Edwards)[J].Journal of Crustacean Biology,1991,11(1):1-9[10] Saigusa M.A substance inducing the loss of premature embryos from ovigerous crabs[J].Boil Bull.1994,186(1)∶81-89[11] Saigusa M,Terajima M,Yamamoto M.Structure,formation,mechanical properties,and disposal of the embryo attachment system of an estuarine crab,Sesarma haematocheir[J].Biol.Bull.,2002,203: 289-306[12] Yang W X,Du N S,Lai W.Studies on the mechanism of egg attachment in Macrobrachium nipponense I.Scanning electron microscopy observation of egg membranes and egg attachment[J]Journal of Hebei University(Natural Science),1996,16(4):34-40.[杨万喜,堵南山,赖伟.日本沼虾卵子附着机制研究I.卵膜及卵子附着的扫描电镜观察.河北大学学报(自然科学报),1996,16 (4):34-40][13] Yang W X,Zhou H.Primart study on the histology of exoskelecton of female pleopod in Eriocheir sinensis[J].Donghai Marine Science,2000,18 (3)∶29-33.[杨万喜,周宏.中华绒螯蟹雌体腹肢外骨骼组织学初步研究.东海海洋,2000,18 (3)∶29-33][14] Kang X J,Li S J,Wang G Z,et al.Studies on the egg membrane variation and egg attachment in Scylla serrata[J].Journal of Fisheries of China,2000,24(6)∶500-503.[康现江,李少菁,王桂忠等.锯缘青蟹卵膜变化与卵子附着研究.水产学报,2000,24(6):500-503][15] Wang J P,Du H G, Li Y K,et al.Analysis on amino acids in roe of some common sea fishes in Zhejiang[J].Pact(Part B:Chemical analysis),2001,37:35-36.[王冀平,杜汉根,李玉葵,等.浙江省常见海水鱼鱼卵中氨基酸成分分析[J].理化检验-化学分册,2001,37(增刊):35-36][16] Yao C L,Wang W N,Wang A L.Advance in the research on the influence of amino acid content variations in the body of Shrimps[J].Marine Sciences,2000,24(9):39-42.[姚翠鸾,王维娜,王安利.虾体内氨基酸含量变化及其影响因素的研究进展[J].海洋科学,2000,24(9):39-42][17] Cheng Y X,Li S J,Wang G Z.Changes in lipid class and fatty acid compositions in the ovaries and hepatopancreas of the mud crab Scylla serrata during the vitellogenesis of the second ovarian maturation[J].Acta Oceanologica Sinica,2001,22(3):66-77.[成永旭,李少菁,王桂忠,等.锯缘青蟹卵黄发生期卵巢和肝胰腺脂类的变化.海洋学报,2001,22(3):66-77][18] Gu T J.Biochemistry[M].4thED.,Beijing:People Hygienic Press,1995,133.[顾天爵.生物化学(第4版).北京:人民卫生出版社,1995,136][19] Teshima S,Kanazawa A.Variation in lipid compositions during the larval development of the prawn(Penaeus japonicus)[J].Mem.Fac.Fish.Kagoshima Univ.,1982,31: 205-212[20] Ji W J.Fatty acid changes during larval development of Penaeus chinensis[J].Journal of Fishery Sciences of China,1996,3 (4):28-34.[季文娟.中国对虾幼体发育各阶段脂肪酸组成的研究.中国水产科学,1996,3 (4):28-34][21] Ward G D,Middle ditch B S,Missler S R,et al.Fatty acid changes during larval development of Penaeus setiferus[J].Proc.World Maricul Soc.,1979,10 :464-471[22] Lytle J S.Polyunsaturated fatty acid profiles as a comparative tool in assessing maturation diets of Penaeus vannamei[J].Aquacult,1990,89(314):287-299[23] Wang J Y, Zhu S G, Xu C F.Biochemistry[M]3rdED.,Beijing:Higher Education Press,2002,82-91.[王镜岩,朱圣庚,徐长法.生物化学(第3版).北京:高教出版社,2002,82-91][24] Kanazawa A,Koshio S.lipid nutrition of the spiny lobster Panulirus japonicus(decapoda,lalinuridae)[J].Crustaceana,1994,67,226-232
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