洞庭湖浮游生物群落DNA指纹与理化因子的关系
RELATIONSHIP BETWEEN DNA FINGERPRINTING OF PLANKTON COMMUNITY AND PHYSICO2CHEMICAL FACTORS
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摘要: 对东洞庭湖7个采样站点的浮游生物群落DNA多态性进行了RAPD指纹分析,并探讨了其DNA指纹与理化因子的关系。结果如下:(1)从80条随机引物中筛选出的11条引物共获得148条谱带,多态率为98.6%;所用引物在各站点可获得45-70条扩增谱带,平均为57.6条,其中Ⅳ站最少;(2)Ⅳ站的泥沙含量最高(75.5mg/L),其他各站在1.2-40.3mg/L;各站点间的pH值比较接近,都呈弱碱性;Ⅰ站的TN、TDN及TP含量最高,Ⅲ站的TDP最高,但各站点相差不大。相似性聚类分析表明,浮游生物群落DNA指纹将7个站点划分为两大类---Ⅳ站作为单独的一类而明显有别于其他6个站点,这与洞庭湖浮游生物分布的主要制约因子---泥沙含量的聚类结果是一致的。因此,研究表明浮游生物群落DNA指纹与环境主要限制因子是密切相关的,这类资料的积累将为建立简易、有效的水资源质量评价体系提供科学依据。Abstract: Water resources are of great importance in maintaining the sustainable development of natural resources. However,with the rapid increase of population and economy, pollution leads to water crisis and other challenges on aquatic ecosystem.Therefore, how to assess water quality rapidly and accurately and maintain the health of aquatic ecosystem has become the focusfor ecological study.In order to offerfacilities and accumulate basic data for water quality assessment, this paper studied the relationship betweengenetic information of plankton community and the major environmental factors. The physico chemical factors of seven samplingstations in Eastern Dongting Lake were measured with general methods and the DNA polymorphism of plankton community wasanalyzed by random amplified polymorphic DNA (RAPD). Then,their relationship was assessed with cluster analysis. The resultswere: (1) 11of 80 screened random primers used in the study generated a total of 148 observable RAPD bands and 98.6 % ofwhich were polymorphic bands; the mumber of amplified bands at different sampling stations ranges from 45 to 70, the meannumber was 57.6 and the station Ⅳgot the minimum; (2) the content of silt at station Ⅳwas the highest (75.5 mg/L) and theother stations were from 1.2 to 40.3 mg/L ; the discrepancy of other five factors measured in the investigation were not obviousin different stations. Cluster analysis based on RAPD markers and the major environmental factor showed that DNAfingerprintingof plankton community was correspondent to the content of silt. And the seven sampling stations could be grouped into two clusters: the station Ⅳwas a single cluster and another cluster consists of the other six stations.In conclusion, the DNAfingerprintingof plankton community was closely related to the environmental physico chemical factors that mainly restrict the distribution of planktonc organisms, and the data of such kind accumulated will play an important rolein constructing one handy and effectual systemfor water quality assessment.
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Keywords:
- Plankton /
- RAPD /
- DNA fingerprinting /
- Physico chemical factors /
- Dongting Lake
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