一种海洋席藻淡化过程中脂肪酸组成的应急变化
FATTY ACIDS STRESS RESPONSES OF A MARINE CYANOPHYTE PHORIDIUM SP. DURING DESALINATION PROCESS
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摘要: 采用三种淡化方式,对一种海洋席藻(Phormidium sp.)淡化培养中脂肪酸组成的应急变化进行了研究.结果表明:游离脂肪酸和结合脂肪酸在整个培养过程中有着完全不同的变化特征.对淡化过程的应急反应,结合脂肪酸首先表现出含量降低而游离脂肪酸含量增加;当藻体开始适应后结合脂肪酸的含量变化开始趋于稳定而游离脂肪酸含量呈现恢复性的降低;当藻体生长状况开始下降时,游离脂肪酸含量立即开始增加.其中游离脂肪酸C18:2(n-6)有着与其他脂肪酸不一样的变化特征,在整个培养过程中都有着持续的增加直至开始死亡,此现象在不更换培养液的方式中尤其明显,认为它是该种席藻生长的自我抑制剂之一.采取换水培养和缓慢淡化的方式,可以部分消减海生席藻淡化过程中脂肪酸等生理生化因子的剧烈变化.Abstract: A marine filamentous, mat-forming cyanobacteria, which was originally isolated from Zhoushan coastal area in 1985, and kept in the Key Laboratory of Marine Biotechnology, Ningbo University, has exhibited potential application in water cleaning. In order to culture a marine cyanophyte Phormidium sp. in fresh water, three modes of desalination were carried out (mode 1, the alga was directly cultured into fresh water, and followed by change of 2/3 volume of culture medium every 3 days; mode 2,the alga was cultured in 16‰ sea water, and desalinated to be 8‰ in the second day, and total fresh water in the third, and followed by change of 2/3 volume of culture medium every 3 days; mode 3, the desalination process was the same as mode 2, except not followed by the change of culture medium). The growth curve experiment showed that the worst growth condition occurred in mode 3 with negative growth and bleaching phenomena. The changes of fatty acids upon the desalination stress were determined using GC-MS. Total lipids were extracted using Bligh-Dyer method, and free fatty acid and bound fatty acids were separated using silica gel chromatography. After saponification and methylation, the fatty acids were subjected to Shimadzu QP2010 gas chromatography mass spectrometer using AOC-20 autosampler, with SPB-50 column (30m×0.25mm×0.25μm). The fatty acid was qualified by retention time and mass spectrometry, and quantified using relative composition with C19∶0 as internal standard. The results indicated that free fatty acids and bound fatty acids change in different patterns. At first 3 days, the bound fatty acids decrease while free fatty acids increase. Then, after the alga begins to accommodate the environmental conditions, the contents of bound fatty acids are stabilized, and the free fatty acids decrease.When the alga enters into senescent stage, the free fatty acids increase rapidly again. Among each individual fatty acid, the change pattern of free fatty acid C18∶2(n-6) is weird, that it increases all the time during the culture period until the death of the alga, especially in the culture mode 3 (no change of culture medium). Therefore, C18∶2 (n-6) is considered as the growth auto-inhibition compound. Based on the research data, it is proposed that frequent culture medium change and prolonged desalination process could alleviate the unwanted effects of radical changes of fatty acids content.
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Keywords:
- Marine cyanophyte /
- Phoridium sp /
- Desalination /
- Fatty acid
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