一种海洋席藻淡化过程中脂肪酸组成的应急变化
FATTY ACIDS STRESS RESPONSES OF A MARINE CYANOPHYTE PHORIDIUM SP. DURING DESALINATION PROCESS
-
摘要: 采用三种淡化方式,对一种海洋席藻(Phormidium sp.)淡化培养中脂肪酸组成的应急变化进行了研究.结果表明:游离脂肪酸和结合脂肪酸在整个培养过程中有着完全不同的变化特征.对淡化过程的应急反应,结合脂肪酸首先表现出含量降低而游离脂肪酸含量增加;当藻体开始适应后结合脂肪酸的含量变化开始趋于稳定而游离脂肪酸含量呈现恢复性的降低;当藻体生长状况开始下降时,游离脂肪酸含量立即开始增加.其中游离脂肪酸C18:2(n-6)有着与其他脂肪酸不一样的变化特征,在整个培养过程中都有着持续的增加直至开始死亡,此现象在不更换培养液的方式中尤其明显,认为它是该种席藻生长的自我抑制剂之一.采取换水培养和缓慢淡化的方式,可以部分消减海生席藻淡化过程中脂肪酸等生理生化因子的剧烈变化.Abstract: A marine filamentous, mat-forming cyanobacteria, which was originally isolated from Zhoushan coastal area in 1985, and kept in the Key Laboratory of Marine Biotechnology, Ningbo University, has exhibited potential application in water cleaning. In order to culture a marine cyanophyte Phormidium sp. in fresh water, three modes of desalination were carried out (mode 1, the alga was directly cultured into fresh water, and followed by change of 2/3 volume of culture medium every 3 days; mode 2,the alga was cultured in 16‰ sea water, and desalinated to be 8‰ in the second day, and total fresh water in the third, and followed by change of 2/3 volume of culture medium every 3 days; mode 3, the desalination process was the same as mode 2, except not followed by the change of culture medium). The growth curve experiment showed that the worst growth condition occurred in mode 3 with negative growth and bleaching phenomena. The changes of fatty acids upon the desalination stress were determined using GC-MS. Total lipids were extracted using Bligh-Dyer method, and free fatty acid and bound fatty acids were separated using silica gel chromatography. After saponification and methylation, the fatty acids were subjected to Shimadzu QP2010 gas chromatography mass spectrometer using AOC-20 autosampler, with SPB-50 column (30m×0.25mm×0.25μm). The fatty acid was qualified by retention time and mass spectrometry, and quantified using relative composition with C19∶0 as internal standard. The results indicated that free fatty acids and bound fatty acids change in different patterns. At first 3 days, the bound fatty acids decrease while free fatty acids increase. Then, after the alga begins to accommodate the environmental conditions, the contents of bound fatty acids are stabilized, and the free fatty acids decrease.When the alga enters into senescent stage, the free fatty acids increase rapidly again. Among each individual fatty acid, the change pattern of free fatty acid C18∶2(n-6) is weird, that it increases all the time during the culture period until the death of the alga, especially in the culture mode 3 (no change of culture medium). Therefore, C18∶2 (n-6) is considered as the growth auto-inhibition compound. Based on the research data, it is proposed that frequent culture medium change and prolonged desalination process could alleviate the unwanted effects of radical changes of fatty acids content.
-
Keywords:
- Marine cyanophyte /
- Phoridium sp /
- Desalination /
- Fatty acid
-
-
[1] Mundt S,Kreitlow S,Nowotny A,et al.Biochemical and pharmacological investigations of selected cyanobacteria [J].In.J Hyg and Environ Health,2001,203:327-334[2] Dumas A,Laliberté G,Lessard P,et al.Biotreatment of fish farm effluents using the cyanobacterium Phormidium bohneri [J].Aqua Eng,1998.17: 57-68[3] Blier R,Laliberté G,de la Noüe J.Tertiary treatment of cheese factory anaerobic effluent with Phormidium bohneri and Micractinium pusillum [J].Biores Technol.1995,52(2):151-155[4] Stadler T,Mollion J,Verdus M-C,et al.Algal biotechnology [M].Barking,UK:Elsevier Applied Science.1988.403-411[5] Lu K H,Lin X.The effect of domestication with seawater and freshwater on fatty acid composition of five species of microalgae [J].Acta Hydrobiol Sin,2001, 25(2):179-184.[陆开宏,林霞.海、淡水驯化对5种微藻脂肪酸组成的影响[J].水生生物学报,2001,25(2):179-184][6] Caizares-Villanueva R O,Domínguez A R,Cruz M S,et al.Chemical composition of cyanobacteria grown in diluted,aerated swine wastewater [J].Biores Technol,1995,51(2-3):111-116[7] Yamada N,Murakami N,Kawamura N,et al.Mechanism of an early lysis by fatty acids from axenic Phormidium tenue(musty odor-producing cyanobacterium)and its growth prolongation by bacteria [J].Biol Pharm Bull,1994,17(9):1277-1281[8] Murakami N,Yamada N,Sakakibara J.An autolytic substance in an axenic cyanobacterium Phormidium tenue [J].Chem Pharm Bull,1990,38(3):812-814[9] Welti R,Weiqi L,Maoyin L.et al.Profiling menbrane lipids in plant stress responses:role of phospholipase in freezing-induced lipid changes in arabidopsis [J].J Biol Chem,2002,277(35):31994-32002[10] Barclay K D,Mckersie B D.Peroxidation reactions in plant membranes:effects of free fatty acids [J].Lipids,1994,29(12):877-883[11] Bligh E G,Dyer W J.A rapid method lipid extraction and purification [J].Can J Biochem Physiol,1959,37:911-923[12] Saito H,Kotani Y,Keriko J M,et al.High levels of n-3 polyunsaturated fatty acids in Euphausia pacifica and its role as a source of docosahexaenoic and icosapentaecoic aceds for higher trophic levels [J].Mar Chem,2002,78:9-28[13] Wang Z F.Qualitative and quantitative analysis of chromatography[M].Beijing:Chemical Industry Press,2000.170-172.[汪正范.色谱定性与定量[M].北京:化学工业出版社,2000,170-172][14] Kayama M,Araki S,Sato S.Lipids of Marine Plants[M].Vol II.Florida:CRC Press,Inc.1989,4-21[15] Ching-Piao Liu,Liang-Ping Lin.Ultrastructural study and lipid formation of Isochrysis sp.CCMP1324 [J].Bot Bull Acad Sin, 2001,42:207-214 Mundt S,Kreitlow S,Nowotny A,et al.Biochemical and pharmacological investigations of selected cyanobacteria [J].In.J Hyg and Environ Health,2001,203:327-334[2] Dumas A,Laliberté G,Lessard P,et al.Biotreatment of fish farm effluents using the cyanobacterium Phormidium bohneri [J].Aqua Eng,1998.17: 57-68[3] Blier R,Laliberté G,de la Noüe J.Tertiary treatment of cheese factory anaerobic effluent with Phormidium bohneri and Micractinium pusillum [J].Biores Technol.1995,52(2):151-155[4] Stadler T,Mollion J,Verdus M-C,et al.Algal biotechnology [M].Barking,UK:Elsevier Applied Science.1988.403-411[5] Lu K H,Lin X.The effect of domestication with seawater and freshwater on fatty acid composition of five species of microalgae [J].Acta Hydrobiol Sin,2001, 25(2):179-184.[陆开宏,林霞.海、淡水驯化对5种微藻脂肪酸组成的影响[J].水生生物学报,2001,25(2):179-184][6] Caizares-Villanueva R O,Domínguez A R,Cruz M S,et al.Chemical composition of cyanobacteria grown in diluted,aerated swine wastewater [J].Biores Technol,1995,51(2-3):111-116[7] Yamada N,Murakami N,Kawamura N,et al.Mechanism of an early lysis by fatty acids from axenic Phormidium tenue(musty odor-producing cyanobacterium)and its growth prolongation by bacteria [J].Biol Pharm Bull,1994,17(9):1277-1281[8] Murakami N,Yamada N,Sakakibara J.An autolytic substance in an axenic cyanobacterium Phormidium tenue [J].Chem Pharm Bull,1990,38(3):812-814[9] Welti R,Weiqi L,Maoyin L.et al.Profiling menbrane lipids in plant stress responses:role of phospholipase in freezing-induced lipid changes in arabidopsis [J].J Biol Chem,2002,277(35):31994-32002[10] Barclay K D,Mckersie B D.Peroxidation reactions in plant membranes:effects of free fatty acids [J].Lipids,1994,29(12):877-883[11] Bligh E G,Dyer W J.A rapid method lipid extraction and purification [J].Can J Biochem Physiol,1959,37:911-923[12] Saito H,Kotani Y,Keriko J M,et al.High levels of n-3 polyunsaturated fatty acids in Euphausia pacifica and its role as a source of docosahexaenoic and icosapentaecoic aceds for higher trophic levels [J].Mar Chem,2002,78:9-28[13] Wang Z F.Qualitative and quantitative analysis of chromatography[M].Beijing:Chemical Industry Press,2000.170-172.[汪正范.色谱定性与定量[M].北京:化学工业出版社,2000,170-172][14] Kayama M,Araki S,Sato S.Lipids of Marine Plants[M].Vol II.Florida:CRC Press,Inc.1989,4-21[15] Ching-Piao Liu,Liang-Ping Lin.Ultrastructural study and lipid formation of Isochrysis sp.CCMP1324 [J].Bot Bull Acad Sin, 2001,42:207-214
-
期刊类型引用(4)
1. 韩琪琪，张杰. 中药对水产动物免疫应答、抗氧化功能及抗病力影响的研究进展. 黑龙江畜牧兽医. 2024(22): 17-22 . 
百度学术
2. 马霞，周延州，郭振环，赵丽，刘永录，王林，张国祖. 玉屏风散与超微粉中毛蕊异黄酮葡萄糖苷体外溶出率的研究. 中国兽药杂志. 2019(11): 38-42 . 百度学术
3. 黄小红，曹岩，江俊勇，马贵华，孔红兴，李达. 饲料中添加纳米硒对草鱼生长性能、免疫器官指数和抗氧化性能的影响. 中国饲料. 2017(16): 30-34 . 百度学术
4. 巩华，刘春花，赵长臣，孙承文，黄志斌，陆文雄，龚钜财. 中草药预混料对罗非鱼生长性能和免疫抗病力的影响. 中国渔业质量与标准. 2017(02): 43-49 . 百度学术
其他类型引用(14)
计量
- 文章访问数: 924
- HTML全文浏览量: 2
- PDF下载量: 449
- 被引次数: 18
下载: