一种海生超微型黄藻的色素蛋白复合体
PIGMENT-PROTEIN COMPLEXES IN A MARINE SPECIES OF XANTHOPHYTA
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摘要: 从海生超微型黄藻品系PP983中分离得到一种水溶性色素蛋白复合物,其光学特性与普遍存在于甲藻叶绿体中的多甲藻素-叶绿素a-蛋白复合物(Peridinin-Chlorophyll a-Protein Complexes, PCP)类似;初步测得该色素蛋白复合体的分子量约为15,200,分子中多甲藻素、叶绿素a、蛋白的摩尔比为16:2:5.研究结果显示,PP983中的PCP是作为聚光色素复合体存在于藻细胞的叶绿体中的.Abstract: A water-soluble photosynthetic light-harvesting complex was being isolated from a marine picoalga strain PP983 of Xanthophyta, grown under mass culture conditions. A crude preparation of this complex was obtained as a brick red supernatant by sonication of a whole cell suspension in 0 1 mol L-1 Tris buffer pH8.4 followed by high speed centrifugation. The extract was purified by 30% and 45%(NH4)2SO4 fractionation and Sephadex G-100 chromatography. The pigment-protein complex is characterized by its absorption spectrum having main absorption maxima at 458nm, 482nm and 670nm, and another maximum at approximately 300 nm by using a SHIMADZU UV-1601 spectrophotometer to scan room spectra ranging from 300 to 750nm. The results of absorption spectra showed that the complex have the characteristics of the peridinin-chlorophyll a-protein complex(PCP) of dinoflagellates. Chromophore analysis, by spectral analysis of extracts and polyamide thin-layer chromatography confirmed the presence of peridinin and chlorophyll a in the complex. Therefore, the peak at 458nm and 670 nm corresponds to Soret band and α band of chlorophyll a respectively, and the maximum at 482 nm is due to peridinin present in the complex. The chromoprotein has a molecular weight of about 154,200 and shows that a peridinin:chlorophyll a:protein ratio is close to 16:2:5 according to spectrophotometric analyses of chromophore content. The result indicated that the peridinin-chlorophyll a-protein complex presumably functions in vivo as photosynthetic lightharvesting pigments.
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[1] Rowan K S. Photosynthetic pigments of algae[M]. New York:cambridge University Press,1989. 124,212-225[2] Lee R E. Phycology(2nd)[M]. New York:Cambridge University Press, 1989.516[3] Hu H H, Dai L F, Dai H P, et al. Amino acid and fatty acid composition of a marine species of Xanthophyceae.Chin. J. Appl. Environ. Biol.1999,5(5):487-491. [胡晗华,戴玲芬,戴和平,等. 一种海生黄藻的氨基酸和脂肪酸组成. 应用与环境生物学报,1999,5(5):487-491][4] Haxo F T,Kycia J H, Somers G F, et al. Peridinin-chlorophyll a proteins of the dinoflagellate Amphidinium carterae(Plymouth 450)[J].Plant physiol, 1976,57:297-303[5] Song P S, Koka P,Prézelin B B, et al.Molecular topography of the photosynthetic light-harvesting pigment complex, peridinin-chlorophyll a-protein, from marine dinoflagellates [J].Biochemistry,1976,15(20):4422-4427[6] Hu H H, Dai L F, Dai H P, et al. Occurrence of Peridinin in a Marine Species of Xanthophyta. Oceanologia et Limnologia Sinica. 2001,32(5):489-493. [胡晗华,戴玲芬,戴和平,等.多甲藻素在一种海生黄藻中的存在. 海洋与湖沼,2001,32(5):489-493][7] Haidak D J, Mathews C K, Sweeney B M. Pigment protein complex from Gonyaulax[J]. Science,1966,156:212-213[8] Roman S J, Govind N S, Triplett E l, et al. Light regulation of peridinin-chlorophyll a-protein(PCP)complexes in the dinoflagellate, Glenodinium sp[J].Plant Physiol,1988,88:594-599[9] Jovine R V M, Triplett E L, Nelson N B, et al. Quantification of chromophore pigments, apoprotein abundance and jsoelectric variants of peridinin-chlorophyll a-protein complexes(PCPs)in the dinoflagellate Heterocapsa pygmaea grown under variable light conditions[J]. Plant Cell Physiol,1992,33(6):733-741[10] Ten Lohuis M R,Miller D J. Light-regulated transcription of genes encoding peridinin chlorophyll a proteins and the major intrinsic light-harvesting complex proteins in the dinoflagellate Amphidinium carterae Hulburt(Dinophycae): Changes in cytosine methylation accompany photoadaption [J]. Plant physiol. 1998,117: 189-196 Rowan K S. Photosynthetic pigments of algae[M]. New York:cambridge University Press,1989. 124,212-225[2] Lee R E. Phycology(2nd)[M]. New York:Cambridge University Press, 1989.516[3] Hu H H, Dai L F, Dai H P, et al. Amino acid and fatty acid composition of a marine species of Xanthophyceae.Chin. J. Appl. Environ. Biol.1999,5(5):487-491. [胡晗华,戴玲芬,戴和平,等. 一种海生黄藻的氨基酸和脂肪酸组成. 应用与环境生物学报,1999,5(5):487-491][4] Haxo F T,Kycia J H, Somers G F, et al. Peridinin-chlorophyll a proteins of the dinoflagellate Amphidinium carterae(Plymouth 450)[J].Plant physiol, 1976,57:297-303[5] Song P S, Koka P,Prézelin B B, et al.Molecular topography of the photosynthetic light-harvesting pigment complex, peridinin-chlorophyll a-protein, from marine dinoflagellates [J].Biochemistry,1976,15(20):4422-4427[6] Hu H H, Dai L F, Dai H P, et al. Occurrence of Peridinin in a Marine Species of Xanthophyta. Oceanologia et Limnologia Sinica. 2001,32(5):489-493. [胡晗华,戴玲芬,戴和平,等.多甲藻素在一种海生黄藻中的存在. 海洋与湖沼,2001,32(5):489-493][7] Haidak D J, Mathews C K, Sweeney B M. Pigment protein complex from Gonyaulax[J]. Science,1966,156:212-213[8] Roman S J, Govind N S, Triplett E l, et al. Light regulation of peridinin-chlorophyll a-protein(PCP)complexes in the dinoflagellate, Glenodinium sp[J].Plant Physiol,1988,88:594-599[9] Jovine R V M, Triplett E L, Nelson N B, et al. Quantification of chromophore pigments, apoprotein abundance and jsoelectric variants of peridinin-chlorophyll a-protein complexes(PCPs)in the dinoflagellate Heterocapsa pygmaea grown under variable light conditions[J]. Plant Cell Physiol,1992,33(6):733-741[10] Ten Lohuis M R,Miller D J. Light-regulated transcription of genes encoding peridinin chlorophyll a proteins and the major intrinsic light-harvesting complex proteins in the dinoflagellate Amphidinium carterae Hulburt(Dinophycae): Changes in cytosine methylation accompany photoadaption [J]. Plant physiol. 1998,117: 189-196
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