鲸类微卫星引物对长江江豚的适用性研究
APPLICABILITY OF CETACEAN MICROSATELLITE PRIMERS IN THE YANGTZE FINLESS PORPOISE
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摘要: 微卫星在长江江豚(Neophocaena phocaenoides asiaeorientalis)中的应用研究还未见报道.本研究采用已发表的来自6个鲸种的23对微卫星引物对一个长江江豚群体DNA样本进行了微卫星扩增.结果表明其中有对引物在此群体中的扩增产物是稳定且多态的,序列分析结果表明这对引物的扩增产物都具有AC或GT两碱基重复单元,从而证明了扩增的有效性.研究结果表明用从其他鲸类分离出的微卫星引物可以快速筛选到适用于长江江豚指纹分析的引物.Abstract: Application of microsatellites in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis) has not been reported.In this study amplification of microsatellites were performed in DNA samples of the Yangtze finless porpoise population by PCR using twenty-three published microsatellite primers from Phocaena phocoena,Eubalaena glacialis,Physeter catodon,Megaptera novaeangliae,Tursiops truncates,Globicephala melas. The results indicated seven primer pairs produced stable and polymorphic PCR products in the population.Further sequencing analysis showed that the amplified product sequences of the seven primer pairs all own AC or GT di-nucleotide repeats,which confirmed that the amplification was valid.The results suggest that applicable primers in fingerprinting analysis of the Yangtze finless porpoise could be obtained faster by screening of microsatellite primers isolated from other cetacean species.
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Keywords:
- The Yangtze finless porpoise /
- Microsatellite /
- Screening
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Basin,2002,11(5):427-432[魏卓,王丁,张先锋,等.长江八里江江段江豚种群数量、行为及其活动规律与保护.长江流域资源与环境,2002,11(5):427-432][6] Zhang X F,Wang K X.Population viability analysis for the Yangtze finless porpoise[J].Acta Ecologica Sinica,1999,19(4):529-533[张先锋,王克雄.长江江豚种群生存力分析.生态学报,1999,19(4):529-533][7] Hilton-Taylor C.IUCN Red list of Threated Species[C].Gland:IUCN-The World Conservation Union,2000[8] Dietrich W,Katz H,Linclon S E.A genetic map of the mouse suitable for typing intraspecific crosses[J].Genetics,1992,131:423-447[9] Yang G,Zhou K Y.Variability of the mitochondrial control region in population of finless porpoise,Neophocaena phocaenoides,in Chinese waters[J].Acta Zoologica Sinica,1997,43(4):411-419[杨光,周开亚.中国水域江豚种群遗传变异的研究.动物学报,1997,43(4):411-419][10] Yang G,Zhou K,Ren W,et al.Population genetic structure of finless porpoises,Neophocaena phocaenoides,in Chinese waters inferred from mitochondrial control region sequences[J].Mar.Mammal.Sci.,2002,18(2):336-347[11] Zane L,Bargelloni L,Patarnello T.Invited 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multiple molecular genetic analyses[J].Proc.Natl.Acad.Sci.USA,1996,93(16):8792-8795[25] Palsboll P J,Allen J,Berube M,et al.Genetic tagging of humpback whales[J].Nature,1997,388(6644):767-769[26] Valsecchi E,Palsboll P,Hale P,et al.Microsatellite genetic distances between oceanic populations of the humpback whale(Megaptera novaeangliae)[J].Mol.Biol.Evol.,1997,14(4):355-362[27] Amos W,Wilmer J W,Fullard K,et al.The influence of parental relatedness on reproductive success[J].Proc.R.Soc.Lond.Ser.B.,2001,268:2021-2027[28] Moller L M,Beheregaray L B,Harcourt R G,et al.Alliance membership and kinship in wild male bottlenose dolphins(Tursiops aduncus)of southeastern Australis[J].Proc.R.Soc.Lond.Ser.B.,2001,268:1941-1947[29] Waldick R C,Kraus S,Brown M,et al.Evaluating the effects of historic bottleneck events:an assessment of microsatellite variability in the endangered,North Atlantic right whale[J].Mol.Ecol.,2002,11(11):2241-2249
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