中华鳖非特异性免疫功能的群体差异研究
A STUDY ON THE VARIATION OF NON-SPECIFIC IMMUNE FUNCTIONS AMONG POPULATIONS OF SOFT-SHELLED TURTLE TRIONYX SINENSIS
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摘要: 从补体总量、红细胞C3b受体花环率、红细胞天然免疫肿瘤细胞花环率、T淋巴细胞活性E花环率及白细胞吞噬活性五方面,探讨了黄河鳖、淮河鳖、洞庭湖鳖、鄱阳湖鳖及太湖鳖在非特异性免疫功能上的差异.主要结果:(1)在补体总量及红细胞天然免疫肿瘤细胞花环率上,五群体之间差异不显著(F淮河鳖>洞庭湖鳖>鄱阳湖鳖>太湖鳖;(3)在T淋巴细胞活性E花环率上,黄河鳖显著高于鄱阳湖鳖(P0.01),其余群体间则差异不显著(P>0.05),花环率大小顺序为:黄河鳖>淮河鳖>洞庭湖鳖>鄱阳湖鳖>太湖鳖;(4)在白细胞吞噬活性上,除淮河鳖与鄱阳湖鳖差异不显著外(P>0.05),其余群体间则差异极显著(F>0.01),活性大小顺序为:黄河鳖>洞庭湖鳖>淮河鳖>鄱阳湖鳖>太湖鳖. 综上所述,在非特异性免疫功能上,黄河鳖最强,太湖鳖较弱.Abstract: The non-specific immune functions were studied among five populations of soft-shelled turtle from Yellow River,Huai River,Dongting Lake,Poyang Lake and Taihu Lake from five aspects--1) the total amount of complement, 2) the percentage of erythrocyte C3b receptor rosette, 3) the percentage of erythrocyte native immune adhering tumor cell rosette, 4)the percentage of erythrocyte active rosette(EaR)of T lymphocyte and 5)the activity of leukocyte phagocyte The major results were:(1) There were no significant difference on the total amount of complement and the percentage of erythrocyte native immune adhering tumor cell rosette among the five populations of soft-shelled turtle (F3b receptor rosette,Yellow River population was significantly higher than that of Poyang Lake population and Taihu Lake population(PHuai River population>Dongting Lake population>Poyang Lake population>Taihu Lake population.(3) On the percentage of EaR of T lymphocyte, Yellow River population was significantly higher than that of Poyang Lake population(P0.01),there was no significant difference among other populations(P>0.05).The order of EaR percentage was Yellow River population>Huai River population>Dongting Lake popuation>Poyang lake population>Taihu Lake population.(4) On the activity of leukocyte phagocyte,there was no significant difference between Huai River population and Poyang Lake population(P>0.05),while there was highly significant defference among other populations(F>0.01).The order of activity was Yellow River population>Dongting Lake population>Huai River population>Poyang Lake population>Taihu Lake population.From above all,it could be concluded that Yellow River population was the highest one on non-specific immune functions while Taihu Lake population was the lowest one.
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L.Identification of an interleukin-2 substance in splenocyte culture supernatant of Grass carp,Ctenopharyngodon idellus and Chinese soft-shelled turtle, Trionyx sinensis [J].Acta Hydrobiologica Sinica,2001,25(1):21-27.[郭琼林.草鱼、中华鳖脾脏细胞培养上清液IL-2物质的检测.水生生物学报,2001,25 (1):21-27][6] Sheng G X,Zhou R L.Modern Immunology Experimental Technolo-gy[M].Hu Bei Science and Technology Press,2002,301-303,429-430.[沈关心,周汝麟.现代免疫学实验技术.武汉:湖北科学技术出版社,2002,301-303,429-430][7] Cai W Q,Sun P F.Resistance of three strains of crucian carps to the outbreak-fish-disease[J].Journal of Fisheries of China.,1993, 17(4):44-51.[蔡完其,孙佩芳.三种鲫鱼对暴发性鱼病的抗病力.水产学报,1993,17(4):44-51][8] Cai W Q, Sun P F.Within species Variation of resistance of common carps in the case of outbreak-fish-disease[J].Journal of Fisheries of China,1994,18(4):290-295.[蔡完其,孙佩芳.三种鲤鱼对暴发性鱼病抗病力的差异.水产学报,1994,18(4):290-295][9] Cai W Q,Sun P F.Among-species variation of resistance of grass carp,black carp,bighead carp and silver carp to the outbreak-fish disease[J].Journal of Fisheries Science of China,1995:2(2):71-77.[蔡完其,孙佩芳."四大家鱼"对暴发性鱼病的抵抗力的种间差异.中国水产科学,1995:2(2):71-77][10] Li Z L.Microbiology and the Assaying Technology[M].Guang zhou:Guang Dong Science and Technology Press,1993,68-69,536-539.[李振林.微生物学及其检验技术.广州:广东科技出版社.1993,68-69,536-539][11] Du N X.Veterinary Immunology[M].Beijing:Chinese Agriculture Press,1995,31,59-66,210-213.[杜念兴.兽医免疫学.北京:中国农业出版社.1995,31,59-66,210-213][12] Guo F,Yu Z Q, Zhao Z P.The preliminary study on erythrocyte immune function [J].Journal of Chinese Medicine,1982,62(12):715-716.[郭峰,虞紫茜,赵中平.红细胞免疫功能的初步研究.中华医学杂志,1982,62(12):715-716][13] Cai W Q,Xuan X R.Variations of erythrocyte immune functions in four populations of red common carp[J].Journal of Fishery Sciences of China,2003,10(2):133-136.[察完其,轩兴荣.红鲤4群体间红细胞免疫功能及其差异[J].中国水产科学,2003,10(2):133-136][14] Chen P Y,Huang Z M.SPSS 10.0 for Windows;Practical Guide [M].Beijing:People's Military Medical Publisher,2002,102-107.[陈平雁,黄浙明.SPSS 10.0统计软件应用教程.北京:人民军医出版社.2002,102-107][15] Cai W Q,Gong X W,Sun P F.Comparison on the function of nonspecificity immunity between Taihu Lake population and Taiwan population of soft-shelled turtle( Trionyx sinensis)[J].Acta Hydrobiologica Sinica,2001,25(1):95-97.[蔡完其,宫兴文,孙佩芳.中华鳖太湖群体和台湾群体非特异性免疫功能比较.水生生物学报,2001,25(1):95-97][16] Xie Z W,Li S H.Complement activity of GIANT PTNDA[J].Acta ZooLogica Sinica,1991,37(1):107-108.[谢秩勋,李素华.大熊猫总补体活性初探.动物学报,1991,37(1):107-108][17] Siegel I.,Lin T L.,Gleichan,N.The red cell immune system[J].Lancet,1981,2(8246):556-559[18] Wang X D,Rao J R.The discovery and studies of the extension erythrocyte phagocytosis[J].Journal of Fisheries of China,1996, 20(1):72-75.[王旭东,饶家荣.红细胞广泛吞噬作用的发现和研究.水产学报,1996,20(1):72-75][19] Bi A H.Medicine Immunology [M].Beijing:People's Military Medical Publisher,1995,44-59,312-315.[毕爱华,医学免疫学.北京:人民军医出版社.1995,44-59,312-315][20] Cai W Q, Li S F,Liu Z Z,et al.Evaluation of aquaculture performance of seven populations of Trionyx sinensis from larvae to adult stage[J].Journal of Fisheries of China.2002,26(5):433-439.[蔡完其,李思发,刘至治,等.中华鳖七群体稚鳖--成鳖阶段养殖性能评估.水产学报,2002,26(5):433-439][21] Shen J Y, Zhang Z Y,Qian d,et al.Studies on humoral immune response of Trionyx sinensis to Aeromonas sobria inactived vaccine[J].Acta Hydrobiologica Sinica,2003,27(1):27-30.[沈锦玉,张志育,钱冬,等.中华鳖对温和气单胞菌体液免疫应答规律的研究.水生生物学报,2003,27(1):27-30]
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