河蟹MTXO细胞的离体培养和细胞学研究
CYTOLOGY AND CULTURE OF NEUROSECRETORY CELL IN THE EYESTALK OF ERIOCHEIR SINENSIS
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摘要: 对河蟹眼柄神经分泌细胞的细胞学进行了研究,建立了河蟹眼柄视神经节终髓X器官(MTXO)细胞原代培养的实用方法.MTXO神经分泌细胞在添加了谷氨酰胺和常量抗菌素的L-15培养基中表现出快速的再生生长,细胞生长可保持3-5d,维持存活约18d.在pH.8-7.8、温度20℃-28℃及渗透压950-1100mOsm条件下,均能存活和生长,但在无Ca2+或添加Ca2+通道阻断剂CdCl2的培养基中不能生长.依据大小、形态、分布和超微结构特征及体外生长方式区分出A、B、C三种不同类型细胞.Abstract: the neurosecretory cells were studied in terms of cytology and primary culture in the MTXO of Eriocheir sinensis, and a practical method for primary culture of peptidergic neurons was set up.The peptidergic neurons, when dissociated from the MTXO, exhibited immediate outgrowth for 3-5 days and survived for 18 days or more in the defined medium supplemented with glutamine and antibiotics.The neurons could survive in some conditions involving changes of pH(7.0- 7.9).temperature(22℃- 28℃) and osmolarity (950- 1100mOsm).The outgrowth of the peptidergic neurons could be restrained in the Ca-free medium and blocked by Cd2+ (Cd2+ current blocker)in the medium.Three types of neurosecretory cells were distinguished on the basis of size, morphology, distribution, form of outgrowth and ultrastructure.There is an ultrastructural evidence that B, C types of neurosecretory cells have a rest phase in the development of Eriocheir sinensis.
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Keywords:
- Crustacea /
- Eriocheir sinensis /
- X-organ /
- Neurosecretory cells /
- Ultrastructure /
- Cell culture
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