6-DMAP抑制栉孔扇贝(♀)×虾夷扇贝(♂)受精卵第一极体释放后染色体的分离状况
CHROMOSOME SEGREGATION IN FERTILIZED EGGSFROM CHLAMYS FARRERI♀×PATINOPECTEN YESSOENSIS♂FOLLOWING INHIBITION OF POLAR BODY1WITH6-DMAP
-
摘要: 三倍体贝类因其具有生长快、个体大、肉质佳和成活率高等优良的经济性状而在水产养殖业中具有重要的应用价值[1]。获得100%三倍体贝类的最佳途径是通过四倍体贝类与二倍体贝类杂交来获得。目前已经发展出多种诱导四倍体的方法,包括抑制受精卵第一极体的释放、抑制受精卵的卵裂、细胞融合和利用人工雌核发育等方法。人工诱导四倍体技术已经在多种鱼类上获得成功[2]。迄今,国内外学者已对多种贝类[3]进行了四倍体育种研究,都获得了一定比例的四倍体胚胎或幼虫,甚至是稚贝,但是除了太平洋牡蛎Crassostreagigas(Thunberg)[4]外大部分由于存活率低最后都没有获得两性能育且形成群体的四倍体贝类。
-
-
[1] Yang A G, Wang Q Y, Zhang Y,et al. Comparison of the growth characters between the triploid and diploid Chlamys (Azumapecten) farreri [J].Marine Sciences, 2000,24(8): 21)23 [杨爱国,王清印,张岩,等.栉孔扇贝三倍体与二倍 体的生长比较.海洋科学, 2000,24(8): 21)23]
[2] Wu P. Research progress of fish polyploidy breeding in China [J].Journal ofShanghaiFisheries University, 2005,14(1): 72)78 [吴萍.我国鱼类多倍体育种的研究进展.上海水产 大学学报, 2005,14(1): 72)78]
[3] Wang Z P, LiK J, Yu R H,etal.Progress of tetraploid breed-ing in molluscs [J].Journal ofOcean University ofChina, 2004,34(2): 195)20[王昭萍,李慷均,于瑞海,等.贝类 四倍体育种研究进展.中国海洋大学学报, 2004,34(2): 195)202]
[4] Guo X, Allen S K Jr. Viable tetraploids in the Pacific oysters (Crassostrea gigasThunberg) produced by inhibiting polarbody ?in eggs from triploids [J].MolecularMarBiolBiotechnology, 1994,3(1): 42)54
[5] GuoX, HershbergerW K, CooerK,etal.Genetic consequences ofblocking polar body?with cytochalasin B in fertilized eggs of the Pacific oyster,Crassostrea gigasò. Segregation of chromo-somes [J].BiolBull, 1992,183: 387)393
[6] Hua Y Q, XiM G, Fu S Z,et al. Chromosome segregation in fertilized eggs from triploid pacific oysters,Crassostrea gigas (Thunberg), following inhibition of polar body?[J].Biol. Bull, 1997,193: 14)19
[7] LiY R, QueHY, ZhangG F. Effectof inhibiting the firstpolar body on chromosome segregation in fertilized eggs and ploidy composition of embryos in the zhikong scallop,Chlamys farreri [J].Marine Sciences, 2005,29(8): 66)70 [李永仁,阙华 勇,张国范.抑制栉孔扇贝第一极体对受精卵染色体行为及 胚胎倍性组成的影响.海洋科学, 2005,29(8): 66)70]
[8] YangH, QueHY, HeY,etal. Chromosome segregation in fer-tilized eggs from zhikong scallopChlamys farrerifollowing polar body I inhibition with CB [J]. JShellfish Res,2000,19(1): 101)105
[9] ChangY Q, WuW M, YangD G. Induced segregation of chro-mosome ofployploid in Patinopecten yessoensiswith cytochalasin B [J].Journal ofDalian Fisheries University, 2001,16(4): 235)240 [常亚青,吴伟明,杨旦光.细胞松弛素B诱导虾 夷扇贝多倍体的受精卵染色体分离状况.大连水产学院学 报, 2001,16(4): 235)240]
[10] HeM X, JiangW G, Pan JP. Chromosome segregation in ferti-l ized eggs from pearl oyster Pinctada martensii Dunker following the firstpolar body inhibition with cytochalasin B [J].Journal ofFisheries ofChina, 2002,26(1): 15)20 [何毛贤,姜卫国, 潘金培. CB抑制合浦珠母贝受精卵第一极体释放的染色体 分离.水产学报, 2002,26(1): 15)20]
[11] Lan G B, XieR C, Yan B,etal.Mechanism of theFormation of Tetraploids of Pinctada martensii Induced with Cytochalasim B [J].GuangxiSciences, 2001,8(3): 204)209 [兰国宝,谢 若痴,阎冰,等.细胞松弛素B诱导马氏珠母贝四倍体形成 机制研究.广西科学, 2001,8(3): 204)209]
[12] LiX, WangZ C, ZhangG F,etal.Studieson themechanism of effects ofmedicines on inducing triploid abalone [J]. Journalof Dalian Fisheries University,1999,14(3): 7)1[李霞,王子 臣,张国范,等.药物诱导皱纹盘鲍三倍体作用机理研究. 大连水产学报, 1999,14(3): 7)12]
[13] Liu S L, YangA G. Healthy breeding techniques ofprimaryma-rinemollusk in China [M]. Beijing: Ocean Press. 2005, 40) 60[刘世禄,杨爱国.中国主要海产贝类健康养殖技术.北 京:海洋出版社. 2005, 40)60]
[14] Zhou LQ, YangA G, Liu Z H,et al.Cytological observations on cross fertilization betweenChlamys farreri(a) andPatin-opecten yessoensis(`) scallops [J]. Chinese Journal ofZoolo-gy,2003,38(4): 20)24 [周丽青,杨爱国,刘志鸿,等. 栉孔扇贝(a)@虾夷扇贝(`)受精细胞学观察.动物学杂 志, 2003,38(4): 20)24]
[15] YangA G, WangQ Y, Liu ZH,etal.The hybrid between the scallopsChlamys farreri and Patinopecten yessoensis and the in-heritance characteristics of its first filial generation [J].Marine FisheriesResearch, 2004,25(5): 1)5 [杨爱国,王清印,刘志 鸿,等.栉孔扇贝与虾夷扇贝杂交及子一代的遗传性状.海 洋水产研究, 2004,25(5): 1)5]
[16] GuerraM. Hematoxylin: a simple, multiple-use dye for chromo-some analysis [J].Genetics andMolecularBiology, 1999,2(1): 77)80
[17] ShuderG, Rieder C L. Centriole number and the reproductive capacity of spindle poles [J].J CellBiol, 1985,100: 887) 896
[18] LiX X. Effects of centrosome on cell division and ploidy during the chromosome manipulation in marine invertebrates [J]. Oceanologia etLimnologia Sinica, 1995,26(4): 443)493 [李 孝绪.论海洋无脊椎动物染色体操作过程中中心体对细胞 分裂和倍性的影响.海洋与湖沼, 1995,26(4): 443)493]
[19] KomaruA, MatsudaH, YamakawaT,etal.Chromosome behav-ior of meiosis inhibited eggs with cytochalasin B in Japanese pearl oyster [J].BullJapSocSciFish, 1990,56: 1419)1422
[20] LongoF J, MathewsL, HedgecockD. Morphogenesis of maternal and paternal genomes in fertilized oyster eggs (Crassostrea gigas): effects of cytochalasin B atdifferent periods duringme-i oticmaturation [J].BiolBull, 1993,185: 197)214
[21] Longo F J, Anderson E. Cytological aspects of fertilization in the lamellibranch,Mytilus edulis.ò. Developmentof themale pro-nucleus and the association of thematernally and paternally de-rived chromosomes [J].JExpZool, 1969,172: 97)120
[22] Longo F J, E Anderson. An ultra-structural analysis of fertiliza-tion in the surfclam,Spisula solidissima.?. Polarbody forma-tion and development of the female pronucleus [J].J Ultra-structRes, 1970,33: 495)514
计量
- 文章访问数: 817
- HTML全文浏览量: 0
- PDF下载量: 441
下载: