胡子鲇、月鳢、泥鳅具气呼吸作用器官呼吸上皮的电镜观察
AN ELECTRON MICROSCOPIC STUDY OF THE RESPIRATORY EPITHELIUM IN THE AIR RESPIRATION ORGANS OF CLARIAS FUSCUS,CHANNA ASIATICA AND MISGURNUS ANGUILLICAUDATUS
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摘要: 应用扫描电子显微镜与透射电子显微镜技术观察了具气呼吸作用的胡子鲇树枝状鳃上器、月鳢耳片状鳃上器和泥鳅的肠等器官,证实3种淡水鱼类具气呼吸作用的器官表面均被覆一层高度毛细血管化的呼吸上皮.胡子鲇的呼吸上皮表面形态呈玉米棒状,月鳢的鳃上器表面为半圆形鹅卵石状,泥鳅肠内壁表面如云朵状.这3种器官的呼吸上皮细胞均为同一种类型,是一类特化的扁平上皮细胞,兼有哺乳类肺泡Ⅰ型和Ⅱ型上皮细胞的细微结构特征.同时,构成气血屏障的三部分即呼吸上皮细胞、毛细血管内皮细胞胞质薄层和两者之间的基底膜与其他脊椎动物的呼吸器官极为相似.
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关键词:
- 胡子鲇 /
- 月鳢 /
- 泥鳅 /
- 气呼吸作用:超微结构
Abstract: The structure of the air respiratory organs of the fishes,Clarias fuscus with arborescent organs,Channa asiatica with ear-shaped labyrinthine organs and Misgurnus anguillicaudatus with air respiratory intestines has been investigated by scanning and transmission electron microscopy. The resuli suggests that the air respiratory organs are covered by a highly vascularized respiratory epithelium on the surface in all three freshwater species.In C. fuscus,the surface of the respiratory epithelium is corn like. The semicircle cobblestone-shaped protrusions are present on the surface of the labyrinthe organs in C. asiatica. M. anguillicaudatus has a cloud-shaped surface in its inner wall of the intestines. All respiratory epithelial cells of three air respiratory organs are the same type which is a kind of modified squamous epitheial cells. ,They show the ultrastrural characteristics of both type Ⅰ and type Ⅱ epithelial cells of lung alveoli in mammals. At the same time,the blood-air barrier is composed of three layers:the thin layer of the extended cytoplasm of respiratory epithelial cells,the thin sheet of cytoplasm of endothelial cells and the basement membrance between them is similar to that of the respiratory organs in other vertebrates.-
Keywords:
- Clarias fuscus /
- Channa asiatica /
- Misgurnus anguillicaudatus /
- Air respiration
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[1] Pan J H,Liu C H,Zheng W B,et al.The morphological structure of the swim bladder of Pangasius sutchi and its air-breathing[J].Zoological Research,1988,9:88-92[潘炯华,刘成汉,郑文彪,等.苏氏NB158的鳔的形态结构和气呼吸习性[J].动物学研究.1988,9:88-92][2] Maina J N.The morphology of the lung of the African lungfish,Protopterus aethiopicus[J].Cell Tissue Res,1987,250:191-196[3] Liu W S.Development of the respiratory swimbladder of Pangasius sutchi.[J].J Fish Biology,1993,42:159-167[4] Zheng W B,Liu W S.Morphology and histology of the swimbladder and ultrastructure of respiratory epithelium in the air-breathing catfish,Pangasius sutchi(Pangasiidae).[J].J Fish Biol,1988,33:147-154[5] Fang Z Q,Zheng W B,Xiao Z,et al.Observation on the fine structure of the gill in Pangasius sutchi.[J].Journal of Fisheries of China,2001,25:489-491.[方展强,郑文彪,肖智,等,苏氏NB158鲇鳃超微结构观察[J].水产学报,2001,25:489-491][6] Zheng W B,Pan J H,Liu W S.Studies on the histological structure of swimbladder and ultramicroscopic structure of respiratory-epithelium in Pangasius sutchi.[J].Acta Hydrobiologica Sinica,1988,12:163-168[郑文彪,潘炯华,刘文生.苏氏NB158鲇鳔的组织学及呼吸上皮的超微结构的研究[J].水生生物学报.1988,12:163-168][7] Hughes G M,Datta Munshi J S.Nature of the air-breathing organs of the Indian fishes Channa,Amphipnous,Clarias and Saccobranchus as shown by electron microscopy.[J].Zool Lond,1973,170:245-270[8] Maina J N and Maloiy G M O.The morphometry of the lung of the African lungfish(Protopterus aethiopicus):its structural-functional correlations.[J].Proc R Soc Lond B,1985,224:399-420[9] Zheng Wenbiao,Pan Jionghua and Liu Wensheng.Culture of Catfish in China.[J].Aquaculture,1988,75:35-44[10] Maina J N,Maloiy G M O.The morphology of the respiratory organs of the African air-breathing catfish(Clarias mossambicus):A light,electron and scanning microscopic study,with morphometric observations.[J].Zool Lond,(A)1986,209:421-445[11] Lu X Y,Huang Z X,and Wang Q.Electron microscopic observations of alveoli development andalveolar epithelium differentiation of mouse lung.[J].Anat Res,2000,22:10-12.[卢晓晔,黄中新,王樯.小鼠肺泡发育与肺泡上皮细胞分化的电镜观察[J].解剖学研究,2000,22:10-12][12] Adamson,Ian Y,Linda Yong.Alveolar type Ⅱ cell growth on a pulmonary endothelial extracellular matrix.[J].Am J Physiol,1996,270(Lung Cell Mol.Physiol.14):L1017-L1022[13] Qu Fan Bu Zhan.New function of alveolar type Ⅱ epithelial cells-defend to lung injury and adjust alveoli immunity.[J].Introduction Japanese Medicine.1997,18:26-27[诹访部章.肺泡Ⅱ型上皮细胞的新功能-对肺损伤的防御和肺泡免疫调节.日本医学介绍.1997,18:26-27(杨友竹译)][14] Wright J R.Clearance and recycling of pulmonary surfactant.[J].Am J Physiol,1990,259:L1-12[15] Jeffrey G,Ramasubbareddy D,Anil B Mukherfee.Surfactant-producing rabbit pulmonare alveolar type Ⅱ cells synthesize and secrete an antlinflammatory protein,uteroglobin.[J].Binche Biophy Res Commu,1992,189:662-669[16] Bai Niao Zheng Dian Ta.The fiberated lung ang alveolar type Ⅱepithelial cells.[J].Introduction Japanese Medicine.[白鸟正典他.肺的纤维化与肺泡Ⅱ型上皮细胞[J].日本医学介绍.1999,20:564-565][17] Meban C.An electron microscope study of the respiratory epithelium in the lungs of the fire salamander(Salamandra salamandra).[J].Anat,1979,128:215-224[18] Hughes G M,Datta Munshi J S,and Ojha J.Post-embryonic development of water-and air-breathing organs of Anabas testudineus(Bloch).[J].J Fish Biol,1986,29:443-450[19] Morris W.Browman and Donald L.Kramer.Pangasius sutchi(Pangasiidae),an air-beathing catfish that uses the swimbladder as an accessory respiratory organ.[J].Copeia,1985,4:994-998 Pan J H,Liu C H,Zheng W B,et al.The morphological structure of the swim bladder of Pangasius sutchi and its air-breathing[J].Zoological Research,1988,9:88-92[潘炯华,刘成汉,郑文彪,等.苏氏NB158的鳔的形态结构和气呼吸习性[J].动物学研究.1988,9:88-92][2] Maina J N.The morphology of the lung of the African lungfish,Protopterus aethiopicus[J].Cell Tissue Res,1987,250:191-196[3] Liu W S.Development of the respiratory swimbladder of Pangasius sutchi.[J].J Fish Biology,1993,42:159-167[4] Zheng W B,Liu W S.Morphology and histology of the swimbladder and ultrastructure of respiratory epithelium in the air-breathing catfish,Pangasius sutchi(Pangasiidae).[J].J Fish Biol,1988,33:147-154[5] Fang Z Q,Zheng W B,Xiao Z,et al.Observation on the fine structure of the gill in Pangasius sutchi.[J].Journal of Fisheries of China,2001,25:489-491.[方展强,郑文彪,肖智,等,苏氏NB158鲇鳃超微结构观察[J].水产学报,2001,25:489-491][6] Zheng W B,Pan J H,Liu W S.Studies on the histological structure of swimbladder and ultramicroscopic structure of respiratory-epithelium in Pangasius sutchi.[J].Acta Hydrobiologica Sinica,1988,12:163-168[郑文彪,潘炯华,刘文生.苏氏NB158鲇鳔的组织学及呼吸上皮的超微结构的研究[J].水生生物学报.1988,12:163-168][7] Hughes G M,Datta Munshi J S.Nature of the air-breathing organs of the Indian fishes Channa,Amphipnous,Clarias and Saccobranchus as shown by electron microscopy.[J].Zool Lond,1973,170:245-270[8] Maina J N and Maloiy G M O.The morphometry of the lung of the African lungfish(Protopterus aethiopicus):its structural-functional correlations.[J].Proc R Soc Lond B,1985,224:399-420[9] Zheng Wenbiao,Pan Jionghua and Liu Wensheng.Culture of Catfish in China.[J].Aquaculture,1988,75:35-44[10] Maina J N,Maloiy G M O.The morphology of the respiratory organs of the African air-breathing catfish(Clarias mossambicus):A light,electron and scanning microscopic study,with morphometric observations.[J].Zool Lond,(A)1986,209:421-445[11] Lu X Y,Huang Z X,and Wang Q.Electron microscopic observations of alveoli development andalveolar epithelium differentiation of mouse lung.[J].Anat Res,2000,22:10-12.[卢晓晔,黄中新,王樯.小鼠肺泡发育与肺泡上皮细胞分化的电镜观察[J].解剖学研究,2000,22:10-12][12] Adamson,Ian Y,Linda Yong.Alveolar type Ⅱ cell growth on a pulmonary endothelial extracellular matrix.[J].Am J Physiol,1996,270(Lung Cell Mol.Physiol.14):L1017-L1022[13] Qu Fan Bu Zhan.New function of alveolar type Ⅱ epithelial cells-defend to lung injury and adjust alveoli immunity.[J].Introduction Japanese Medicine.1997,18:26-27[诹访部章.肺泡Ⅱ型上皮细胞的新功能-对肺损伤的防御和肺泡免疫调节.日本医学介绍.1997,18:26-27(杨友竹译)][14] Wright J R.Clearance and recycling of pulmonary surfactant.[J].Am J Physiol,1990,259:L1-12[15] Jeffrey G,Ramasubbareddy D,Anil B Mukherfee.Surfactant-producing rabbit pulmonare alveolar type Ⅱ cells synthesize and secrete an antlinflammatory protein,uteroglobin.[J].Binche Biophy Res Commu,1992,189:662-669[16] Bai Niao Zheng Dian Ta.The fiberated lung ang alveolar type Ⅱepithelial cells.[J].Introduction Japanese Medicine.[白鸟正典他.肺的纤维化与肺泡Ⅱ型上皮细胞[J].日本医学介绍.1999,20:564-565][17] Meban C.An electron microscope study of the respiratory epithelium in the lungs of the fire salamander(Salamandra salamandra).[J].Anat,1979,128:215-224[18] Hughes G M,Datta Munshi J S,and Ojha J.Post-embryonic development of water-and air-breathing organs of Anabas testudineus(Bloch).[J].J Fish Biol,1986,29:443-450[19] Morris W.Browman and Donald L.Kramer.Pangasius sutchi(Pangasiidae),an air-beathing catfish that uses the swimbladder as an accessory respiratory organ.[J].Copeia,1985,4:994-998
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