饲料中添加中性蛋白酶对青鱼生长、消化及鱼体组成的影响
EFFECT OF ADD ING NEUTRAL PROTEASE TO D IETS ON GROW TH PERFORMANCE, D IGESTION, AND BODY COM POSITION OF FINGERL ING BLACK CARP (M YLOPHARYNGODON PICEUS)
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摘要: 用含中性蛋白酶8000U/g的酶制剂，制作酶制剂使用量分别为0、0.5‰、1‰、2‰和3‰的5种实验饲料，喂养平均初始重为(3.03±0.04)g的5组三重复的青鱼鱼种8周。实验在容积为80L的15只循环式水族箱中进行。实验水温为(25.0±0.5)℃。结果表明:实验饲料中酶制剂含量为0.5‰时，实验鱼的鱼体增重与摄食不含酶制剂饲料的实验组无显著差异(p＞0.05)，而当饲料中酶制剂含量提高至1‰时，鱼体增重出现显著提高(p＜0.05)。但饲料中酶制剂含量从1‰进一步提高至2‰和3‰，则鱼体增重不再有显著变化(p＞0.05);饲料中酶制剂含量为1‰、2‰和3‰实验组的摄食量显著高于饲料中酶制剂含量为0和0.5‰的实验组(p＜0.05)。而饲料中酶制剂含量为1‰、2‰和3‰实验组的饲料系数显著低于饲料中酶制剂含量为0和0.5‰的实验组(p＜0.05);青鱼饲料中使用酶制剂对饲料干物质的表观消化率、实验鱼的肠蛋白酶活性、肠和肝胰脏淀粉酶活性、全鱼营养组成、脏体指数、肝体指数无显著影响(p＞0.05);饲料中0.5‰-3‰酶制剂含量时，实验鱼对饲料蛋白质的表观消化率均显著高于饲料中不含酶制剂的实验组(p＜0.05)。青鱼鱼种肝胰脏蛋白酶活性随着饲料中酶制剂使用量从0增加0.5‰和1‰而呈逐渐升高的趋势，但饲料中酶制剂使用量继续增加则不再有显著变化。因此，在本实验条件下，饲料中使用1‰-3‰的中性蛋白酶制剂促进青鱼鱼种的生长和降低饲料系数。Abstract: neutral protease productwith an activity of 8000 u/g was used in the experiment. A basal diet without exogenous enzyme inclusion and four test diets adding 0.5‰, 1‰, 2‰ and 3‰ of the enzyme product respectivelywere prepared to feed five triplicate groups of fish with an initial average body weight of (3.03 ±0.04) g. The feeding trial was conducted in 15 re circulating tank systems (80L each) at a controlled water temperature of 25 ±0.5 ℃ and lasted for eight weeks. At the end of eightweeks, the weight gain of fish fed a diet with 0.5‰ of enzyme product showed no significant difference with that of fish fed basal diet (p >0.05). When fish fed a diet with 1‰ of enzyme product, the weight gain increased significantly (p 0.05). However, the apparent digestibility of dietary protein of fish fed four test dietswere significantly higher than that of fish fed the basal diet (p <0.05). The hepato-pancreas protease activity increased with increasing dietary supplementing the enzyme product up to 1‰, but above this level, it appeared to keep stable. In conclusion, under the condition of this experiment, adding 1‰ -3‰ neutral protease product to fingerling black carp diet could promote feed ingest and digest capacity of dietary protein, and as a result, improve fish growthand feed conversion.
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