Abstract: By using microbial community PFU method, partitioning of organic chloride pollutants such as A-HCH、 B-HCH、C-HCH、 D-HCH、 HCBppc-DDE in different phases of water in Donghu lake in Wuhan city has been studied. Organic chloride pollutants such as A-HCH、 B-HCH、 C-HCH、 D-HCH、 HCB、 ppc-DDE in PFU(polyurethane foam unit) extrusion were detected by GC-ECD after pretreatment by sequential extraction method including cel- l fixing、 supersonic and digest procedures. A cel- l fixing stepwas designed before solvent extraction to prevent the releaseof organic chloride pollutants in cells because of solvent toxicity.During cel- l fixing and digest procedure, the number of microorganisms was detected microscopically in a simulation experiment of Tetrahymena thermophila BF5.The result shows that cel- l fixing can prevent microorganisms bursting into fragment, and microorganisms will disappear thoroughly after digest.The detected results show that main part of organic chloride pollutants enriched inmicro-organisms or strong adsorb on aqueous particles, including life and non-life detritus, minority slightly adsorb on aqueousparticles, and little is in solution. As a sampling material to determine organic chloride pollutants in natural water body, PFU has the merit of high enrichment coefficient and convenience. Becausethe particles absorbed by PFU can be controlled below certain dimension,we suggest that PFU can be used as a sampling method for research of POPs in particles of natural water body.