硫酸盐还原菌对汞的甲基化作用及其影响因子
MICROBIAL METHYLATION OF MERCURY AND THEIR AFFECTING FACTORS IN AQUATICENVIRONMENT
-
摘要: 从受氯碱化工废水严重污染的湖北鸭儿湖1号氧化塘底泥中分离获得了硫酸盐还原菌,研究了其生理特性和环境因子对其生长的影响。并在实验室条件下建立了模拟厌氧水环境,通过正交实验获得汞甲基化的最佳条件,研究了该条件下硫酸盐还原菌在好氧和厌氧状况下对汞的甲基化作用,以及非生物甲基化作用。同时又分别作了单因素实验,并用高效液相色谱法测定了水样中不同形态的汞。结果显示,该硫酸盐还原菌营厌氧生活,在35℃、pH70、07%的盐度、05g/LFe2+和不含硫化物等条件下,可达到最佳生长状态。水环境中汞的甲基化作用主要发生在有微生物为媒介的厌氧环境下,汞的非生物甲基化作用和好氧环境下的甲基化作用均可忽略不计。厌氧环境下,水体温度、pH值、硫化物和盐度等诸多环境因素对汞的微生物甲基化作用的影响也进行了研究与讨论。Abstract: Sulfate-reducing bacteria(SRB)was isolated from the sediments of the oxidation pond of Ya-Er Lake hubei, a heavily polluted lake by direct discharge of chloralkali effluent, and incubated in the laboratory. Its physiology characteristics and the effects of environmental factors were studied.The results show that temperature, pH, salinity, ferrous iron and sulfide greatly affect their characteristics.The effects on bivalent mercury methylation of SRB in the simulative anaerobic aquatic system were investigated.The methylation conditions were optimized by the orthogonal experiment.Themercury methylations of SRBunder anaerobic and aerobic environment werestudied, as well as its abioticmethylation.And we also studied the effects on methylation of the single environmental factor.The mercury compounds were detected by HPLC. In this paper,we developed a simple and rapid method for in situ preconcentration of inorganic and organicmercury compounds in water samples.At the same time, this developed mothod was indicated to be applicable for detectingHg2+and MeHg in environmental water samples in this test.The results indicated that this SRB grows best under the optimum conditions, such as 35 e, pH510, 017% salinity, 015g/L ferrous iron, and without sulfide.Mercury methylation in aquatic environment mostly occurs under anaerobic condition, and is mediated by SRB. And the abiotic and aerobicmercury methylations are negligible. It has been found that microbial methylation in anaerobicaquatic environment is influenced by a wide variety of environmental factors.Thetemperature(35 e), pH(510) and salinity(017%) are themost important affecting factors for mercury methylation.In aquatic environment, Mercury may be one of the most hazardous contaminants. Its ecological and toxicological effects strongly dependent on its chemical species present. Species distribution and transformation processes in natural aquatic systems are controlled by various physical, chemical, and biological factors.Under the environmental conditions, inorganicmercury speciesmay be converted to many times more toxicmethylated forms such as methylmercury.So it is of great necessity and importance for us to study the microbial methylation process and their affecting factors in aquatic environment.
-
Keywords:
- Mercury /
- Methylation /
- Sulfate -reducing bacteria /
- Aquatic environmental factors
-
-
[1] EricD S, YoramC, ArthurM W, Environmental distribution andtransformation of mercury compounds[J].Critical reviews in environmental science and technology, 1995,26(1):1-43
[2] Xu X Q, DengG Q, Hui J Y, et al.Heavymetal pollution insediments from the three gorge reservoir area[J].Acta hydrobiologica sinica,1999, 23(1):1-10[徐小清, 邓冠强, 惠嘉玉, 等.长江三峡库区江段沉积物的重金属污染特征, 水生生物学报, 1999, 23(1):1-10]
[3] Tetsuo H, Hisamitsu N, Yoshitada Y, et al.Formation, distribution, and ecotoxicityof methylmetals of Tin, Mercury, andArsenic inthe evironment[J].Critical reviewsin environmental science and technology,1995, 25(1):45-91
[4] SusanneM U, Trevor W T, Svetlana A.Mercury in the aquatic envronment:a review of factors affecting methylation[J].Critical reviewsin environmental science and technology, 2001, 31(3):241-
[5] Robinson J B,Tuovinen O H,Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds-physiological,biochemical and genetic analyses[J].Microbiol.Reviews, 1984, 48:95
[6] Gilmour C C.,HenryE A.,Mitchell R, Sulfatestimulation of mercury methylation in freshwater sediments [J].Environ.Sci.Technol.,1992, 26:2281
[7] Wright D R,Hamilton R D, Release of methylmercury from sediments:effects of mercury concentration, low temperatureand nutrient addition[J].Can.J.Fish.Aquat.Sic., 1982, 39:1459
[8] Callister SM,WinfreyM R.Microbial methylation of mercury in upper Wisconsin River sediments[J].Water, Air, and Soil Pollution, 1986,29:453
[9] Korthals E T, Winfrey M R.Seasonal and spatial variations in mercury methylation and demethylation in an oligotrophiclake[J].Appl.Environ.Microbiol, 1987, 53:2397
[10] LeeY H,Hultberg H.Methylmercury in some Swedish surface waters[J].Environ.Toxicol.Chem., 1990, 9:833
[11] Fitzgerald W F,Mason R P, Vandal G M, Atmosphericcycling and airwater exchangeof mercuryover mid-continental lacustrineregions[J].Water, Air, and Soil Pollution, 1991, 56:745
[12] Amyot M, Lean D R S, Poissant L, Distribution and transformation of elemental mercury in the St.Lawrence River and Lake Ontario[J].Can.J.Fish.Aquat.Sci., 2000, 57:155
[13] Miller D R, The roleof humicacids in theuptake and release of mercury by freshwater sediments [J], Verb.Internat.Verein Limnol.1975,19:2082
[14] Compeau G C, Bartha R, Effect of salinity on mercury-methylating activity of sulfate-reducing bacteria in estuarinesediments[J] Appl.Environ.Microbiol., 1987, 53:261
[15] Compeau G, BarthaR,Methylation anddemethylation of mercuryunder controlled redox, pH and salinity conditions[J].Appl.Environ.Micro biol., 1984, 48:1203
[16] Regnell O,Tunlid A, Laboratory study of chemical speciation of mercury in lake sediment and waterunder aerobicand anaerobic conditions[J], Appl.Environ.Microbiol., 1991, 57:789
-
期刊类型引用(13)
1. 杨涵，龚艺，甄鹏，谢明花，刘敏，周于琳，李熙银，周莉，桂建芳，王忠卫. 六个繁育场异育银鲫的克隆系鉴定及特征分析. 水生生物学报. 2025(02): 158-168 . 
本站查看
2. 彭飞，方弟安，周彦锋，唐永凯，徐东坡，刘凯，苏胜彦，尤洋. 中华绒螯蟹三龄群体的生物学可塑性初探. 浙江大学学报(农业与生命科学版). 2022(01): 86-95 . 百度学术
3. 黄茂坤，赖谱富. 人工养殖蓝圆鲹肌肉营养成分的分析与评价. 武汉轻工大学学报. 2022(06): 44-52 . 百度学术
4. 刘海平，刘乐乐. 基于SOM模糊识别的鲤科鱼类与鲶形目鱼类氨基酸营养价值研究. 高原科学研究. 2021(01): 1-7 . 百度学术
5. 蒋文枰，贾永义，刘士力，程顺，迟美丽，郑建波，李飞，顾志敏. 鲌鲂F_1、F_2及其亲本肌肉营养成分的比较分析. 浙江农业学报. 2020(07): 1166-1175 . 百度学术
6. 许元峰，胡炜. 大鳞副泥鳅、泥鳅和北方泥鳅肉质比较分析. 水生生物学报. 2020(06): 1222-1229 . 本站查看
7. 程小飞，宋锐，徐远琴，袁希平，李成，伍远安，邓大青，李鸿，李金龙. 网箱养殖刺鲃肌肉营养成分分析及评价. 现代食品科技. 2019(06): 245-250+236 . 百度学术
8. 颜孙安，姚清华，林香信，胡兵，林虬. 不同养殖密度瓦氏黄颡鱼肌肉营养成分分析与评价. 食品安全质量检测学报. 2019(19): 6637-6644 . 百度学术
9. 杨东辉，吴坤杰，杨治国，伦峰. 生态养殖黄鳝与野生黄鳝营养成分和氨基酸含量及重金属元素残留比较分析. 黑龙江畜牧兽医. 2018(13): 199-202 . 百度学术
10. 张君，陈露，余鹏，周清妍，杨小丽，周伟尚，沈保平，苏时萍，万全，桂建芳. 中华鳖4个品系营养成分分析与比较. 水生生物学报. 2018(04): 770-778 . 本站查看
11. 王永明，史晋绒，谢碧文，唐瑞，王淯，齐泽民. 不同年龄段养殖宽体沙鳅肌肉营养成分分析与评价. 水生生物学报. 2018(03): 542-549 . 本站查看
12. 王桢璐，姚东林，郑钧，邹记兴，周爱国. 三种鲤亚科鱼类的肌肉营养成分分析与评价. 水产养殖. 2017(07): 39-42 . 百度学术
13. 李忠，梁宏伟，王忠卫，邹桂伟，桂建芳. 四倍体异育银鲫新品种“长丰鲫”肌肉品质和营养成分分析. 水生生物学报. 2016(04): 853-858 . 本站查看
其他类型引用(1)
计量
- 文章访问数:
- HTML全文浏览量: 0
- PDF下载量:
- 被引次数: 14
下载: