静止和充气培养条件下短期紫外辐射对钝顶螺旋藻光化学效率的影响
SHORT2TERM EFFECTS OF SOLAR ULTRAVIOLET RADIATION ON THE PHOTOCHEMICAL EFFICIENCY OF SPIRULINA PLATENSIS IN NON 2AERATED AND AERATED CULTURES
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摘要: 为了研究短期太阳紫外辐射对钝顶螺旋藻的影响,作者将静止和充气培养的藻体,暴露在全波长太阳辐射PAB(PAR+UVA+UVB),去除UVB辐射PA(PAR+UVA)及切断所有紫外辐射的光合有效辐射P(PAR)三种光处理条件下,测定了其光化学效率的变化。结果表明,紫外辐射(UVP)及光合有效辐射(PAR)均能导致钝顶螺旋藻的光化学效率降低,表现出了明显的光抑制,但是,UVR可导致更大程度的光抑制。充气培养条件下,与早晨(07:00)初始值相比,PAR导致了11%~20%的光抑制,而UVR(PAB-P)所产生的额外光抑制占9%~31%;静止培养条件下,UVR的存在使得螺旋藻的光化学效率趋近于零(无法检出)。在两种培养条件下,藻细胞所受最大光抑制均发生在中午,下午(17:00)表现出不同程度的恢复。紫外辐射使得类胡萝卜素及藻蓝蛋白与叶绿素a含量的比例增大。与PAB和P相比,PA处理使得螺旋藻类胡萝卜素和藻蓝蛋白与叶绿素含量之比明显升高,UVA可能会诱导类胡萝卜素及藻蓝蛋白的合成。Abstract: Continuous depletion of the stratospheric ozone layer results in increasing solar ultraviolet radiation(UVR) reaching theearth’ s surface. Which has been considered to affect living organisms. Solar UVR may also affect a variety of physiological andbiochemical processes in the economic cyanobacterium, Spirulina platensis. However,little has been documented on this aspect.The present study evaluated the short2term effects of UVRon photochemical efficiencyof S. platensis in aerated and non2aeratedcultures while exposing it to full2spectrum solar radiation(PAB),solar radiation depleted of UVB(PA)and solar radiation deprivedof total UV(P:PAR).It was found that both UVR and PAR inhibited the photochemical efficiency during noontime(11:00213:00),however,UVR+PAR(PAB) resulted in higher inhibition. In aerated cultures, PAR resulted in 11 %～20 % compared to the initial value in themorning (07:00),UVR(PAB2P) brought about 9 %～31 % extra photoinhibition. While in non2aerated cultures,the photochemi2cal effciency could not be detected when exposed to UVR. The greatest inhibition was observed at noontime, and the recoverywas achieved in late afternoon(17:00) in both the aerated and non2aerated cultures. UVR increased the ratio of carotenoids orphycocyanin to chlorophyll a. Compared with PAB and P treatments, cells exposed to PA showed higher values of the pigmentratios,indicating a possibility that UVA may enhance the synthesis of carotenoids and phycocyanin. Although S. platensis wasnegatively affected by UVR, it tended to develop mechanisms counteracting the damaging effects of UVR during this short2termexposure period, such as down2regulation of the photosynthetic activity of accumulation of the carotenoids.
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[1] Chinese Algal Society. Studies on Spirulina [M]. Qingdao:QingdaoNews press.1997,7[中国藻类学会. 螺旋藻资料汇编. 青岛:青岛新闻出版社.1997,7]
[2] Hu HJ. Current statuses and emerging prospects of the biotechnologyof Spirulina in overseas[J]. Journal of Wuhan Botanical Research.1997,15(4):360-374[胡鸿钧. 国外螺旋藻生物技术的现状及发展趋势,武汉植物学研究.1997,15(4):360-374]
[3] Hu HJ.Biology and biotechnologyof Spirulina [M].Beijing:SciencePress.2003,10. [胡鸿钧. 藻类生物学及生物技术原理. 北京:科学出版社.2003,10]
[4] Xia J R,Gao K S. Studies on photoinhibition of Spirulina maximagrown in high CO2concentration [J]. Acta Hydrobiologica Sinica.2002,26(1):14-18. [夏建荣,高坤山. 高浓度 CO2培养条件下极大螺旋藻光抑制的研究. 水生生物学报. 2002,26(1):14-18]
[5] Xia J R,Gao K S. Effects on high CO2concentration on growth andphotosynthesisof Spirulina maxima [J]. Acta Hydrobiologica Sinica.2001,25(5):474-480. [夏建荣,高坤山. 高浓度 CO2对极大螺旋藻生长和光合作用的影响. 水生生物学报. 2001,25 (5):474-480]
[6] Li Y G,Hu HJ,Zhang L J,Chen Z X. Studies on CO2supply tech2nique for Spirulina production [J]. Journal of Wuhan Botanical Re2search.1996,14(4):349-356. [李夜光,胡鸿钧,张良军,陈志祥. 以 CO2为碳源工业化生产螺旋藻工艺艺术的研究. 武汉植物学研究.1996,14(4):349-356]
[7] Li Y G, Hu HJ,G ong X M. Studies on the mechanism of pH valuechange and carbon conversion ratio of Spirulina media [J]. ChineseJournal of Biotechnology.1996,13:242-248. [李夜光,胡鸿钧,龚晓敏. 螺旋藻培养液pH变化的机理和碳源利用率的研究. 生物工程学报.1996,13:242-248]
[8] Richmond A, Grobbelaar J U. Factors affecting the output rate ofSpirulina platensis with reference to mass cultivation [J]. Biomass.1986,10:253-264
[9] Vonshak A, Guy R. Photoadaptation,Photoinhibition and productivityin the blue2green alga,Spirulina platensis grown outdoors [J]. PlantCell Eviron.1992,15:613-616
[10] Smith R C,Prézelin B B, Baker KS, et al.Ozone depletion:ultravi2olet radiation and phytoplankton bilology in Antarctic waters [J]. Sci2ence 1992,255:952-959
[11] Li G C, Song L R. Effects of ultraviolet radiation on cyanobacterialcells activity [J]. Journal of Microbiology, 2002,22(4):31-33[李国才,宋立荣. 紫外线辐射对蓝藻细胞活性的影响. 微生物学杂志.2002,22(4):31-33]
[12] Yu J,Tang X X,Li YQ. Effect of UV2B radiation on marine microal2gaes [J]. Marine Sciences,2002,26(2):6-8[于娟,唐学玺,李永祺.紫外线2B 辐射对海洋微藻生长效应. 海洋科学. 2002,26(2):6-8]
[13] Qi Y Z,Huang C J,Y ing Z H,Qian F. The ecological effects of UVradiation on a toxic dinoflagellate Alexandrium tamarense [J], O2ceanologia &Limnologia Sinica. 1997,28(2):113-120. [齐雨藻,黄长江,应浙鸿,钱锋. 紫外光对有毒甲藻塔玛亚历山大藻的生态学效应. 海洋与湖沼.1997.28(2):113-120]
[14] Underwood GJ G,Nilsson C, Sundb-ck K, et al. Short2term effectsof UVB radiation on chlorophyll fluorescence,biomass,pigments,andcarbohydrate reactions in a benthic diatom mat [J]. J. Phycol.1999,35:656-666
[15] H-der D2P. Effects of solar UV2B radiation on aquatic ecosystems[J]. Adv. Space Res.2000,26:2029-2040
[16] Rajagopal S, Murthy S D S,Mohanty P. Effect of ultraviolet2B radia2tion on intact cells of the cyanobacterium Spirulina platensis: charac2terization of the alterations in the thylakoid membranes [J] 1J1 Photochem1 Photobiol1B: Biol.2000,54:61-66
[17] Rajagopla S,Jha I B,Murthy S D S,Mohanty P. Ultraviolet2B effectson Spirulina platensis cells:modificationof chromophore2protein inter2action and energy transfer characteristics of phycobilisome [J].Biochem1Biophy1Res1Communications11998,249:1722177
[18] Vonshak A,T orzillo G, T omaselli L. Use of chlorophyll fluorescenceto estimate the effect of photoinhibition in outdoor cultures of Spiruli2na platensis [J]. J. Appl. Phycol.199416:31-34
[19] Vonshak A,T orzillo G,Accolla P, et al. Light and oxygen stress inSpirulina platensis(cyanobacteria-grown outdoors in tubular reactors[J]1 Physiol. Plant.1996,97:175-179
[20] Lu C M,Vonshak A. Photoinhibition in outdoor Spirulina platensiscultures assessed by polyphasic chlorophyll fluorescence transients[J]. J. Appl. Phycol.1999,11:355-359
[21] Figueroa F L,Salles S, Aguilera J, et al. Flores2Moya A,AltamiranoM. Effectsof solar radiationon photoinhibition and pigmentation in thered alga Porphyra leucosticta [J]. Mar. Ecol. Prog. Ser.1997,151:81-90
[22] Underwood GJ C,Nilsson C,Sundb-ck K,Short2term effects of UVBradiation on chlorophyll fluorescence,biomass,pigments,and carbohy2drate reactions in a benthic diatom mat [J]. J. Phycol. 1999,35:656-666
[23] Krause GH,Weis E. Chlorophyll fluorescence and photosynthesis:thebasics [J]. Annu. Rev. Plant Physiol. Plant mol. Biol. 1991. 42:313-349
[24] Parsons T R,StricklandJ D H.Discussionof spectrophotomertic deter2mination of marine plant pigments,with revised equationfor ascertain2ing chlorophylls and carotenoids [J]. J. Mar. Res. 1963,21:155-163
[25] Siegelman H W,Chapman D J,Cole WJ,The bile pigments of plants[J]. Biochem. Soc. Symp.1968,28:107-120
[26] Osmond C B,Chow W S. Ecology of photosynthesis in the sun andshade:summary and prognostications [J]. J. Phycol.1988,15:1-9
[27] Campbell D, Eriksson M2J, ; quist G, et al. The cyanobacteriumSynechococcus resists UV2B by exchanging photosystem Ⅱreaction2center D1 proteins [J]. Proc. Natl. Acad. Sci.1998,95:364-369
[28] Sass L,Spetea C,Máté Z, et al. Repair of UV2B induced damage ofphotosynthesis Ⅱvia de novo synthesis of the D1 and D2 reactioncenter subunits in Synechocystis sp. PCC 6803 [J]. Photosyn. Res.1997,54:55-62
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