非洲爪蟾短型肽聚糖识别蛋白基因的克隆与鉴定

齐志涛; 高谦; 黄贝; 昌鸣先; 聂品

非洲爪蟾短型肽聚糖识别蛋白基因的克隆与鉴定

齐志涛^1,2,3,
高谦¹,
黄贝^1,3,
昌鸣先¹,
聂品^1, ,

中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉 430072
2. 盐城工学院海洋技术系, 江苏省沿海池塘养殖生态重点实验室, 盐城 224051
3. 中国科学院研究生院, 北京 100049

基金项目:

国家自然科学基金资助项目(30830083)

中国科学院创新基金(KSCX2-YW-N-021)资助

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- 文章访问数: 1133
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出版历程
- 收稿日期: 2009-06-03
- 修回日期: 2010-03-17
- 发布日期: 2010-09-24

CLONING AND IDENTIFICATION OF A SHORT TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN IN XENOPUS TROPICALIS

QI Zhi-Tao^1,2,3,
GAO Qian¹,
HUANG Bei^1,3,
CHANG Ming-Xian¹ and
NIE Pin^1, ,

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072;
2. Department of Ocean Technology, Yancheng Institute of Technology, Key Laboratory of Aquaculture and Ecology of Coastal pool of Jiangsu Province, Yancheng 224051;
3. Graduate University of the Chinese Academy of Sciences, Beijing 100049

摘要

摘要: 采用生物信息学方法首次对非洲爪蟾短型肽聚糖识别蛋白(xePGRP-S)基因进行了克隆,并对其在胚胎发育和成年爪蟾各组织中的表达状况进行了分析。xePGRP-ScDNA全长720bp,开放阅读框为549bp,编码182个氨基酸。序列比对显示xePGRP-S与其他物种PGRP-S的序列相似性在42.4%-50.5%之间。RT-PCR显示在非洲爪蟾胚胎发育至3d时可以明显检测到xePGRP-S的表达,之后呈持续性表达,且在所检测的心、肝、脾、肺、肾、肠和胃这7种组织器官中呈组成型表达。
- 非洲爪蟾 /
- 肽聚糖识别蛋白 /
- 克隆 /
- 同源性 /
- 组织表达
Abstract: A short type peptidoglycan recognition protein (PGRP-S) was first cloned from the amphibian model animal Xenopus tropicalis by using the bioinformatics method, and the expression pattern of xePGRP-S in early developing stage and normal xenopus was also analyzed. The xePGRP-S cDNA was 720 bp in length, containing a 549 bp open reading frame encoding 182 a.a. Multiple sequence alignment showed that the xePGRP-S shared 42.4%-50.5% sequence identities with other PGRP-S. RT-PCR showed that xePGRP-S mRNA was not detected in the early development stages (3-48h) until the third day. In the normal adult xenopus, xePGRP-S mRNA was constitutively expressed in all the detected tissues including heart, liver, spleen, lung, kidney, intestine and stomach.
- Xenopus tropicalis /
- Peptidoglycan recognition protein /
- Clone /
- Identity /
- Tissue expression

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参考文献(16)

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文章访问数: 1133
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非洲爪蟾短型肽聚糖识别蛋白基因的克隆与鉴定

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CLONING AND IDENTIFICATION OF A SHORT TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN IN XENOPUS TROPICALIS

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