鳡鱼仔稚鱼耳石的标记和其日轮的确证
OTOLITH MARKING AND DAILY INCREMENT VALIDATING IN LARVAL AND JUVENILE YELLOWCHEEK CARP,ELOPICHTHYS BAMBUSA
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摘要: 用茜素络合物对鳡鱼仔鱼进行了浸泡标记,耳石上能检测到橘红色荧光标记环。100mg/L茜素络合物溶液的标记效果较差;120mg/L和150mg/L溶液浸泡后微耳石的标记率很高,矢耳石和星耳石的标记率低。标记后耳石上的生长轮数与饲养天数间呈一一对应关系,相关方程为:N=0.2663+0.9276D(n=68,r2=0.9664)。方程的斜率0.9276与1无显著差异,证明生长轮确系日轮。鱼体长与微耳石、矢耳石及星耳石的直径间呈显著的线性关系,相关方程为:BL=66.8723LD+2.7064(n=73,r2=0.8867),BL=22.7839SD+6.6066(n=49,r2=0.8525),BL=47.6079AD+3.5660(n=71,r2=0.9012)。Abstract: A total of 74 wild larvae of yellowcheek carp (Elopichthys bambusa) were collected in the Honghu section of the Yangtze River on May 16, June 3 and 13,1999.The larvae collected on May 16 were immersed in 100mg/L alizarin complexone (ALC) solution for 24 hours, then in 120mg/L ALC solution for 24 hours seven days later.The larvae collected on June 3 and 13 were immersed in 150mg/L ALC solution for 24 hours.Under UV light, scarlet-pink fluorescent zones were observed on otoliths.Only a few otoliths could be marked when immersed in 100mg/L ALC solution.When immersed in 120mg/L and 150mg/L ALC solutions, 91.3% and 100% lapillus, 44.83% and 58.06% sagitta, and less than 10% as-tariscus were marked, respectively.The relationship between increments in lapillus (N) and the rearing days (D) could be expressed as N = 0.2663 + 0.9276D (n = 68, r2 = 0.9664).The slope 0.9276 was not significantly different from 1.That means the increments in lapillus formed daily in larval and juvenile stages of the fish.The relationships between body length (BL) and diameters of lapillus (LD), sagitta (SD), astariscus (AD) could be expressed as BL= 66.8723LD+2.7064 (n = 73, r2 = 0.8867), BL= 22.7839SD + 6.6066 (n = 49, r2 = 0.8525) for, and BL= 47.6079AD+ 3.5660 (n =71, r2 = 0.9012), respectively.
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