饲料中不同维生素C含量对长吻的影响
EFFECTS OF D IFFERENT D IETARY VITAM IN C LEVELS ON CHINESE LONGSNOUT CATFISH (LEIOCASSIS LONGI ROSTRIS GΒNTHER)
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摘要: 用维生素C含量为38、364和630mg／kg的三种饲料饲喂初体重为(23.19±0.58)g的长吻8个月，实验结果表明，38mg／kgVC饲料组饲喂的长吻表现出了典型的VC缺乏症状:体色发黑，脊椎侧弯，鳍边由开始的萎缩直到腐烂、贫血、特定生长率显著降低(p＜0.05)。并且38mg／kgVC饲料组长吻的生理指标也受到影响:血清溶菌酶活性显著下降(p＜0.05)，肝脏的SOD活性及MDA含量均显著升高(p＜0.05)，血清皮质醇没有显著差异(p＞0.05)，但各处理组长吻肝脏中均未检测到诱导型HSP70的存在。因此，相对于皮质醇和HSP70，血清溶菌酶、肝脏SOD活性、肝脏MDA含量以及血液理化指标(血红细胞数及血红蛋白)更能反映出VC缺乏下长吻的生理状态。Abstract: Vitamin C is an essential micro-nutrient formost fish and plays an important role in immunity and physiology. The deficiency of dietary vitamin C can result in the damage on fish. The study on the deficiency symptom and the sensitive indicator of vitamin C could provide useful information for fish feed industry1 This experimentwas conducted to investigate the effects of dietaryVitamin C levels on growth performance, immune and physiological responses in Chinese longsnout catfish (Leiocassis longirostris G黱ther), which is an high value aquaculture fish in China. Three practical diets containing 38, 364 and 630mg/kg vitamin C were fed to Chinese longsnout catfish (23119 ?158g) for 8 months1 Vitamin C was used in the form of L-ascorbyl-2-polyphosphate1 The results indicated that the Chinese longsnout catfish in 38mg/kg vitamin C groups showed typical vitamin C deficiency symptom: skin color turning darker, spinal deformities and scoliosis, erosion of fins, anaemia1 SGR and the serum lysozyme activities decreased significantly (p p p >0105). The inductive HSP70 in liver was not detected by western2blotting in all groups. In conclusion, serum cortisol and hepatic HSP70 were not the sensitive indicators for vitamin C deficiency symptom, but the serum lysozyme, hepatic SOD activity, hepatic MDA content and the haematological index (red blood cell number and hemoglobin content) were the more reliable makers to indicate the physiological status in Chinese longsnout catfish.
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Keywords:
- Vitamin C /
- Vitamin C deficiency symptom /
- Chinese longsnout catfish
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