三种水华蓝藻对不同磷浓度生理响应的比较研究
COMPARATIVE STUDIES ON PHYSIOLOGICAL RESPONSES AT PHOSPHORUS STRESS OF THREE WATERBLOOM FORMING CYANOBACTERIA
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摘要: 本实验研究了铜绿微囊藻(MicrocystisaeruginosaFACHB469)、水华鱼腥藻(Anabaenaflos-aquaeFACHB245)和浮游颤藻(OscillatoriaplanctonicaFACHB708)对磷浓度变化的生理响应。结果表明,在缺磷条件下,A.flos-aquae对低磷环境的适应能力较强,O.planctonica其次,M.aeruginosa最差;在磷充足条件,微囊藻对磷过量吸收的能力明显高于其他两种蓝藻。三种蓝藻胞外碱性磷酸酶活性(APA)与培养基中磷浓度呈负相关性,其产生碱性磷酸酶(AP)的能力由高至低为:A.flos-aquaeO.planctonicaM.aeruginosa。磷缺陷时A.flos-aquae产生的胞外APA约是M.aeruginosa的10倍,是O.planctonica的5倍。Abstract: In this paper, the physiological responses of the three waterbloom2forming cyanobacteria (Microcystis aeruginosa FACHB469, Anabaena flos-aquae FACHB245 and Oscillatoria planctonica FACHB708) at phosphorus limitation in stationary cultures were studied. The phosphorus uptake rate is hyperbolic correlated to the extracellular nutrient concentration as described by the Michaelis2Menten equation. Kmfor phosphorus of M. aeruginosa, A. flos-aquae and O. planctonica was 81260, 21199 and 41926, respectively, which indicated that the tolerant ability to the phosphorus stress was in the decreasingorder of A. flos-aquae, O. planktonica and M. aeruginosa in the phosphorus deficient condition. The capacity to luxuriously uptake phosphorus of M. aeruginosa was higher than that of O. planctonica and A. flos-aquae. This result was consistent with the phosphorus uptake kinetics experimentsof the three cyanobacteria. Alkaline phosphatase acitivity (APA) of cyanobacteria were inversely correlated with external phosphorus concentration. The APA was obviously promoted under phosphorus deficiency stress. The capacity of excreting AP in the three cyanobacteria was significantly different under the same phosphorus limited conditions (A. flos-aquae > O. planctonica > M. aeruginosa). Extracellular APA of A. flosaquae was 10 and 5 times higher than that of M. aeruginosa and O. planctonica, respectively, when they were cultured in phosphorus deficient condition. The studies compared phosphorus uptake kinetics and capacityof excreting AP in the three cyanobacteria, which would help to understand the population succession of cyanobacteria in the environmental waterbodies.
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[1] Hecky R E, K ilham P. Nutrient limitation of phytoplankton in fresh-water and marine environments: A review of recent evidence on theeffects of Enrichment [J]. Limnology and Oceanography, 1988,33(4, part 2): 796-822
[2] Ahlgren G. Phosphorus as growth2regulating factor relative to otherenvironmental2factors in cultured algae [J].Hydrobiologia, 1988,170: 191-210
[3] Andersson G, Berggren H, Cronberg G, Gelin C. Effects of plank2tivorous and benthivorous fish on organisms and water chemistry inEutrophic Lakes [J]. Hydrobiologia, 1978, 59(1): 9-15
[4] Schindler D W. Whole2lake eutrophication experiments with phospho2rus,nitrogen and carbon [J]. International Association of Theoreticaland Applied Limnology, 1975, 19: 3221-3231
[5] Schindler D W. Evolutionof phosphorus limitation in lakes [J]. Sci2ence, 1977, 195: 260-262
[6] Liu YD,Fan X,Hu Z Y. Research of Phycology in China [M]. Science Press. 2001 [刘永定, 范晓, 胡征宇主编. 中国藻类学研究. 科学出版社. 2001]
[7] Wetzel R G. Limnology: lake and river ecosystems [M]. Academicpress. 2001
[8] Rose C, Axler R P. Usesof alkaline phophatase activity in evaluatingphytoplankton community phosphorus deficiency [J]. Hydrobiologia,1997, 361: 145-156
[9] Jansson M, Olsson H, Pettersson K. Phosphatase: origin, characteristics and function in lakes [J]. Hydrobiologia, 1988, 170: 157-175
[10] OH SJ, Yamamoto T, Kataoka Y, et al. Utilization of dissolved organic phophorus by the two toxic dinoflagellates,Alexandriumtamarense and Gymnodinium catenatum (Dinophyceae) [J].Fisheries Science, 2002, 68: 416-424
[11] Arnon D I. Copper enzymes in isolated chloroplasts: polyphenoloxi2-dase in Beta vulgaris [J]. Plant physiology, 1949, 24: 1-15
[12] State Environmental Protection Administration of China. Guide ofanalysis methodsfor water and wastewater [M]. (3rdedition.). Beijing: Publishing House of Environmental Science. 2002, 246 [国家环保总局. 水和废水监测分析方法. 第4 版. 北京: 中国环境科学出版社. 2002, 246]
[13] Berman T. Alkaline phosphatase and phosphorus availability in LakeK inneret [J]. Limnology and Oceanography., 1970, 15: 663-674
[14] Sorokin C. Dry weight, packed cell volume and optical density [A].In: Stein J (Eds.), Handbook of phycological methods, culturemethods and growth measurements [C]. London: Cambridge University Press. 1973, 327
[15] Gao X Q, RenJ C, Z ong Z X, Cai XM. Studies on the nutrient energetics of Microcystis aeruginosa [J]. Acta Scientiarum NaturaliumUniversitatis Pekinensis, 1994, 30(40): 461-469 [高学庆, 任久长, 宗志祥, 蔡晓明. 铜绿微囊藻营养动力学研究. 北京大学学报(自然科学版), 1994, 30(40): 461-469]
[16] Zhou PJ, Shen H, LinJ, et al. K inetic studies on the effects of organphosphorus pesticides on the growth of Microcystis aeruginosa anduptake of the phosphorus forms [J]. Bulletin of Environmental Contamination and Toxicolog., 2004, 72: 791-797
[17] Perry MJ. Phosphate utilization by an oceanic diatom in phosphoruslimited chemostat culture and in the oligotrophic waters of the centralNorth Pacific [J]. Limnology and Oceanography, 1976, 21: 88-107
[18] Suttle C A, Harrison PJ. Ammonium and phosphate uptake kineticsof size-fractioned plankton from an oligotrophic freshwater lake [J].Journal of Plankton Research, 1988, 10: 133-149
[19] Zhang C, Z ouJ Z. Nutrient uptake kinetics and growth under nutrientlimitation of pseudonitzschia [J]. Oceanologia et Limnologia Sinica,1997, 28(6): 599-603 [张诚, 邹景忠. 尖刺拟菱形氮磷吸收动力学以及氮磷限制下的增殖特征. 海洋与湖沼, 1997, 28(6): 599-603]
[20] Perry M J. Phosphate uptake by phytoplankton in the Central NorthPacific Ocean [J]. Deepsea Research, 1981, 28(A): 39-49
[21] Jiao N Z. Marine microplankton nitrogen uptake kinetics with specialreference to size-fractionation [J]. Oceanologia et Limnologia Sinica, 1995, 26(2): 191-198 [焦念志. 海洋浮游生物氮吸收动力学及其粒级特征. 海洋与湖沼, 1995, 26(2): 191-198]
[23] Gao G, Gao X Y, Qin B Q, Ji J. Experimental study on the PO34-P threshold of the alkaline phosphatase activity in Taihu Lake [J].Journal of Lake Sciences, 2000, 12 (4): 353-358 [高光, 高锡芸, 秦伯强, 季江. 太湖水体中碱性磷酸酶的作用阈值. 湖泊科学, 2000, 12(4): 353-358]
[24] Hernández I, FernándezJ A, Niell F X. Influence of phosphorus status on the seasonal variation of alkaline phosphatase activity in Porphyra umibilicalis (L.) Kützing [J]. Journal of Experimental MarineBiology and Ecolog, 1993, 173: 181-198
[25] Hernández I, Christmas M, Whitton B A. Testing the allometric scaling of alkaline phosphatase activity to surface/ volume ratio in benthicmarine macrophytes [J]. Journal of Experimental Marine Biology andEcolog y, 1999, 241: 1-14
[26] Nalewajko C, Murphy T P. Effectsof temperature, and availabilityofnitrogen and phosphorus on the abundance of Anabaena and Microcystis inLake Biwa, Japan: an experimental approach [J]. Limnology, 2001, 2: 45-48
[27] Dong Y X. Research on blue algae plankton bloom in Erhai Lake[J]. Yunnan Environmental Science, 1999, 18(4): 28-31 [董云仙. 洱海蓝藻水华研究. 云南环境科学, 1999, 18(4): 28-31]
[28] Tan X P, Min H. Analysisof eco2environmental problems and controlcountermeasure in Qiandao Lake [J].Environmental Pollution &Control., 2004, 6(3): 200-203 [谭湘萍,闵怀. 千岛湖生态环境主要问题及保护对策. 环境污染与防治,2004, 6(3): 200-203]
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