三种水华蓝藻对不同磷浓度生理响应的比较研究
COMPARATIVE STUDIES ON PHYSIOLOGICAL RESPONSES AT PHOSPHORUS STRESS OF THREE WATERBLOOM FORMING CYANOBACTERIA
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摘要: 本实验研究了铜绿微囊藻(MicrocystisaeruginosaFACHB469)、水华鱼腥藻(Anabaenaflos-aquaeFACHB245)和浮游颤藻(OscillatoriaplanctonicaFACHB708)对磷浓度变化的生理响应。结果表明,在缺磷条件下,A.flos-aquae对低磷环境的适应能力较强,O.planctonica其次,M.aeruginosa最差;在磷充足条件,微囊藻对磷过量吸收的能力明显高于其他两种蓝藻。三种蓝藻胞外碱性磷酸酶活性(APA)与培养基中磷浓度呈负相关性,其产生碱性磷酸酶(AP)的能力由高至低为:A.flos-aquaeO.planctonicaM.aeruginosa。磷缺陷时A.flos-aquae产生的胞外APA约是M.aeruginosa的10倍,是O.planctonica的5倍。Abstract: In this paper, the physiological responses of the three waterbloom2forming cyanobacteria (Microcystis aeruginosa FACHB469, Anabaena flos-aquae FACHB245 and Oscillatoria planctonica FACHB708) at phosphorus limitation in stationary cultures were studied. The phosphorus uptake rate is hyperbolic correlated to the extracellular nutrient concentration as described by the Michaelis2Menten equation. Kmfor phosphorus of M. aeruginosa, A. flos-aquae and O. planctonica was 81260, 21199 and 41926, respectively, which indicated that the tolerant ability to the phosphorus stress was in the decreasingorder of A. flos-aquae, O. planktonica and M. aeruginosa in the phosphorus deficient condition. The capacity to luxuriously uptake phosphorus of M. aeruginosa was higher than that of O. planctonica and A. flos-aquae. This result was consistent with the phosphorus uptake kinetics experimentsof the three cyanobacteria. Alkaline phosphatase acitivity (APA) of cyanobacteria were inversely correlated with external phosphorus concentration. The APA was obviously promoted under phosphorus deficiency stress. The capacity of excreting AP in the three cyanobacteria was significantly different under the same phosphorus limited conditions (A. flos-aquae > O. planctonica > M. aeruginosa). Extracellular APA of A. flosaquae was 10 and 5 times higher than that of M. aeruginosa and O. planctonica, respectively, when they were cultured in phosphorus deficient condition. The studies compared phosphorus uptake kinetics and capacityof excreting AP in the three cyanobacteria, which would help to understand the population succession of cyanobacteria in the environmental waterbodies.
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