日本血吸虫卵黄培养细胞超微结构动态的研究
ULTRASTRUCTURE OF CULTURED VITELLINE CELLS FROM ADULT SCHISTOSOMA JAPONICA
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摘要: 从超微结构水平研究日本血吸虫卵黄细胞培养的动态。在体外培养过程中,卵黄细胞对外界环境的改变比日本血吸虫的其它体细胞更加敏感。随着培养时间的延长,卵黄细胞发生不同程度的变性,成熟卵黄细胞比未成熟卵黄细胞先发生变性。变性主要表现在核和胞质的电子密度降低;卵黄球相互融合,或卵黄球与膜之间的空隙逐步增大,最终卵黄球从中释放出来,变成裸露体;脂滴数目增多,体积增大;粗面内质网扩大和囊泡变,其上的核糖体脱颗粒等。由此认为,未成熟卵黄细胞在体外培养过程中超微结构的变化可以作为评价培养条件优劣的一个指标。Abstract: The cultured vitelline cells from adult Schistosoma japonica were observed ultrastructurally.The cells were more sensitive than somatic cells to the changes of in vitro cultured condition, and degenerated in different degrees along with the prolongation of culture.The degeneration was observed earier in mature vitelline cells than in imrnature ones.Changes in the cell structure caused by the degeneration were examined with the following characteristics: the electron density of cells became lower,vitelline globules were fused with each other, the space between vitelline globules and the membrane became larger gradually, vitelline globules were released and uncovered at last.The number and volume of lipid droplets increased.Rough-surfaced endoplasmic reticula enlarged and became vacuolar, and ribosomes left the surface of them.Therefore it was considered that ultrastructural changes of cultured immature vitelline cells could be used to estimate whether the cultured conditions were appropriate.
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Keywords:
- Schistosoma japonica /
- Culture cell of vitelline /
- Ultrastructure /
- Dynamic change
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[1] Bayne C J, Menino J S. Hobbs D J. et al. In vitro cultivation of cells from larval Schistosoma mansoni.[J] J. Parasitology, 1994, 80(1):29-35[2] Hobbs D J. Fryer S E. Duimstra J R. et al. Culture of cells from juvenile worms of Schistosoma mansoni.[J] J. Parasitology, 1993, 79(6):913-921[3] 董惠芬蒋明森李瑛等.日本血吸虫细胞培养方法初探[J].水生生物学报,1995,19(4):382-383[4] 董惠芬蒋明森李瑛等.日本血吸虫培养细胞形态的初步观察[J].见:中国动物学会寄生虫专业学会编.中国动物学会寄生虫专业学会成立10周年纪念论文集.北京:中国科技出版社,1995,131-133.[5] 董惠芬蒋明森李瑛等.日本血吸虫成虫细胞培养条件的初步研究.[J].中国血吸虫病防治杂志,1995,7(5):257-261[6] 董惠芬蒋明森陈晓蓓等.PHA诱导日本血吸虫成虫培养细胞增殖的研究[J].中国人兽共患病杂志,1998,14(5):41-44[7] 董惠芬陈晓蓓蒋明森等.日本血吸虫童虫培养细胞的超微结构观察[J].寄生虫与医学昆虫学报,1998,5(4):211-216[8] 董惠芬蒋明森杨明义等.日本血吸虫成虫培养细胞的超微结构观察[J].动物学报,1999,45(1):1-7[9] 董惠芬蒋明森陈晓蓓等.日本血吸虫童虫培养细胞超微结构动态变化的研究[J].中国寄生虫病防治杂志,1999,12(2):140-142[10] 华先欣周述龙.体外培养日本血吸虫成虫生殖器官超微结构的观察[J].水生生物学报,1988,12(3):236-240[11] 周述龙杨孟样孔楚豪等.在超微结构水平初步观察日本血吸虫雌虫生殖系统[J].武夷科学,1992,9:147-151 Bayne C J, Menino J S. Hobbs D J. et al. In vitro cultivation of cells from larval Schistosoma mansoni.[J] J. Parasitology, 1994, 80(1):29-35[2] Hobbs D J. Fryer S E. Duimstra J R. et al. Culture of cells from juvenile worms of Schistosoma mansoni.[J] J. Parasitology, 1993, 79(6):913-921[3] 董惠芬蒋明森李瑛等.日本血吸虫细胞培养方法初探[J].水生生物学报,1995,19(4):382-383[4] 董惠芬蒋明森李瑛等.日本血吸虫培养细胞形态的初步观察[J].见:中国动物学会寄生虫专业学会编.中国动物学会寄生虫专业学会成立10周年纪念论文集.北京:中国科技出版社,1995,131-133.[5] 董惠芬蒋明森李瑛等.日本血吸虫成虫细胞培养条件的初步研究.[J].中国血吸虫病防治杂志,1995,7(5):257-261[6] 董惠芬蒋明森陈晓蓓等.PHA诱导日本血吸虫成虫培养细胞增殖的研究[J].中国人兽共患病杂志,1998,14(5):41-44[7] 董惠芬陈晓蓓蒋明森等.日本血吸虫童虫培养细胞的超微结构观察[J].寄生虫与医学昆虫学报,1998,5(4):211-216[8] 董惠芬蒋明森杨明义等.日本血吸虫成虫培养细胞的超微结构观察[J].动物学报,1999,45(1):1-7[9] 董惠芬蒋明森陈晓蓓等.日本血吸虫童虫培养细胞超微结构动态变化的研究[J].中国寄生虫病防治杂志,1999,12(2):140-142[10] 华先欣周述龙.体外培养日本血吸虫成虫生殖器官超微结构的观察[J].水生生物学报,1988,12(3):236-240[11] 周述龙杨孟样孔楚豪等.在超微结构水平初步观察日本血吸虫雌虫生殖系统[J].武夷科学,1992,9:147-151
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