高浓度CO2对莱茵衣藻光系统Ⅱ能量流和能量利用效率的影响
INFLUENCES OF EXTERNAL HIGH CO2CONCENTRATION ON THE ENERGY FLUXES AND YIELD OF PSII IN THE GREEN ALGA CHLAMYDOMONAS REINHARDTII
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摘要: 研究了短期内高浓度CO2培养下莱茵衣藻光系统Ⅱ行为的变化,结果表明高浓度CO2培养导致叶绿素a、b和类胡萝卜素含量明显增加,同时随培养时间的延长叶绿素a荧光动力学(快相)曲线中O、J、I、P期的荧光强度明显增加。与空气培养的细胞相比,高浓度CO2在J期的相对可变荧光均维持在较高水平;单位反应中心复合体吸收的能量在高浓度CO2培养6小时后有明显的增加,但单位反应中心捕获和用于电子传递的能量并没有明显地增加。高浓度CO2培养使光系统Ⅱ最大光化学效率、传递的电子能引起的光化学反应效率和电子传递的量子效率均明显低于通空气培养的细胞。以上结果表明不同CO2浓度培养导致莱茵衣藻叶绿素a荧光动力学快相的变化与其光系统Ⅱ功能的变化密切相关,也可能与HCO3转运过程中能量需求的变化有关。Abstract: Eukaryotic algae exhibit marked differences in photosynthetic carbon metabolism dependent on the CO2concentrationduring growth. The lower affinity for inorganic carbon, high CO2compensation and decreased carbonic anhydrase in high CO2con-centration have been reported. However, little attention has been paid to the response of PSII to elevated CO2concentration in a-lgae. Chlorophyll a fluorescence has been widely used to study thePSII behavior under different stress conditions which results inthe establishment of different physiological states. A suitable cell model to study the CO2effects in algae is the protist Chlamy-domonas reinhardtii. To investigate the influences of external high CO2concentration on the energy fluxes and yield of PSII, thechanges of PSII behavior in thegreen alga Chlamydomonas reinhardtii cells exposed to high CO2concentration for 12h were stud-ied by analyzing the chlorophyll a fluorescence transients. High CO2concentration resulted in significant increase by 16.1%,13.7%and 13.9%in the content of chlorophyll a, b and carotenoids, respectively. The polyphasic fluorescencetransients(O-J--IP)was measured by using Plant Efficiency Analyser (PEA) after the cells were dark-adapted, and showed that the level at O, J, Iand P in high-CO2-grown cells significantly raised with the increasing exposure time compared with air-grown ones. The relativevariable fluorescence at the phase J (VJ) maintained a higher value in high-CO2-grown cells in contrast to air-grown ones. Thespecific energy flux for absorption(ABS/RC) after cells were cultured in high CO2concentration (4%CO2) for 6 h had a markedincrease,whereas the specific energy fluxes for electron transport (ETO/RC) and trapping (TRo/RC)were not affected by high CO2concentration. The maximal quantum yield of primary photochemistry (UPo),the efficiencywith which a trapped exciton can movean electron into the electron transport chain further than QA (W O), and the quantum yield of electron transport (UEo) were lower inhigh-CO2-grown cells than in air-grown ones. These results in this study suggested that the difference of chlorophyll a fluores-cence reported here reflected the changes of PSII function which may be associated with active transport of HCO3. The analysisof polyphasic chlorophyll a fluorescence transients was a powerful tool to study the changes of the energy fluxes and yield of PSIIin high CO2concentration.
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Keywords:
- Photosystem Ò /
- JIP -test /
- Energy fluxes /
- Chlamydomonas reinhardtii
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