镉、铜和锌胁迫下黑藻活性氧的产生及抗氧化酶活性的变化研究
GENERATION OF ACTIVE OXYGEN AND CHANGE OF ANTIOXIDANT ENZYME ACTIVITY IN HYDRILLA VERTICILLATA UNDER Cd,Cu AND Zn STRESS
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摘要: 研究了不同Cd、Cu、Zn处理浓度对黑藻体内活性氧()产生及对抗氧化酶(SOD、POD、CAT)活性的分子毒理学效应以探讨高等水生植物抗氧化酶对重金属胁迫的反应。结果表明,三种重金属都不同程度地加快了产生速率;Cu使SOD、POD、CAT活性下降;Cd也都减弱了SOD和POD活性,而CAT活性在0.5—5mg/L处理浓度时增加;Zn对SOD活性也为抑制作用,当浓度为0.5—5mg/L时POD和CAT活性都上升。关联度分析发现Cd、Cu和Zn胁迫下黑藻起主要保护作用的分别为SOD、POD和CAT,而SOD最易受到影响。Cd、Cu处理下的叶绿素含量也都呈下降趋势,而0.5—5mg/L的Zn浓度刺激了叶绿素合成。所有Zn处理、0.5mg/L的Cu处理和0.5—1mg/L的Cd处理的叶绿素a/b值都大于对照值。除了Cu使可溶性蛋白含量减少外,0.5—5mg/L的Zn和0.5—1mg/L的Cd都使其含量增加。综合起来,Cu的毒性最强,其次为Cd,Zn最弱。致死阈浓度分别为:Cu:0.5—1mg/L;Cd:1—2mg/L;Zn:5—6mg/L。SOD是评价重金属对沉水植物毒性效应的灵敏指标。黑藻对水环境Cu污染反应敏感。Abstract: It is well known that the unbalance between production and elimination of the active oxygen often caused by manychemicals and the antioxidant systems play an important role in the resistance to the stress. Heavy metal is a major pollutant inwater body,but little work has been done on the response of antioxidant enzyme activity in aquatic plants to heavy metals. In orderto investigate the molecular toxicological effects of heavy metals on antioxidant enzyme activity in higher hydrophytes, Hydrillaverticillata(L. f.) Royle,a wide spread submerged macrophyte in China is selected and cultivated in nutrient solution containingdifferent concentration of Cd,Cu,Zn for 7days. The results showed that three heavy metals accelerated the generation rate of tocertain extent ;Cu inhibited the SOD,POD and CAT activity ;Cd decreased the SOD and POD activity,while CAT activity in creased under 5mg/L ;Zn had a similar influence with Cu and Cd on SOD,but both POD and CAT activity were enhanced by015-5mg/L concentration. Evaluation with the method of grey relational grade analysis suggested that POD, POD and CATplayed an important role in protection against the injury resulting from active oxygen under Cu,Cd and Zn stresses,respectively.SOD was the most susceptible to the adversity. Chlorophyll content decreased when treated with Cd and Cu,and augmented whenZn concentration was 015-5mg/L. Chlorophyll a/b value was higher than that of the control under all Zn concentration ;015mg/L Cu and 015-1mg/L Cd treatment. The soluble protein content ascended when treated with 015-5mg/L Zn and 015-1mg/LCd,but descended by all Cu treatment. In general,the toxicity of Cu was the most serious following by Cd and Zn. The lethal concentration was 015-1mg/L for Cu |1-2mg/L for Cd and 5-6mg/L for Zn. Therefore, SOD activity served as a sensitivebiomarker to evaluate the toxic effect of heavy metal on submerged macrophytes and H. verticillata was impressible to the Cu pollution in water environment.
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Keywords:
- Cd /
- Cu /
- Zn /
- Stress /
- Hydrilla verticillata /
- Active oxygen /
- Antioxidant enzyme /
- Toxicity
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