Tandeau de Marsac N,de la Torre F,Szulmajster,J.Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2[J].Mol.Gen.Genet,1987,209:396—398[2] Xu X,Kong R,Hu Y.High larvicidal activity of intact recombinant cyanobacterium Anabaena sp.PCC 7120 expressing Gene 51 and Gene 42 of the Bacillus sphaericus 2297[J].FEMS Microbiol.Lett,1993,107:247—250[3] Lu F,Devi K S,Chitinis VP,et al.Molecular genetics of cyanobacteria:new avenue in biotechnology[J].J.Sci.Indust Res,1996,55:555—563[4] 董桂蕃.卫生杀虫剂应用技术[M].北京:化学工业出版社,1998[5] Liu J W,Yap W H,Thanabalu T,et al.Efficient synthesis of mosquitocidal toxins in Asticcacaulis excentricus demonstrates potential of gram-negative bacteria in mosquito control[J].Nature Biotechnology,1995,14:343—347[6] 黎尚豪.固氮蓝藻作为晚稻肥源的研究[J].水生生物学集刊,1981,7:417—423[7] Schnepf E,Crickmore N,Rie J V,et al.Bacillus thuringiensis and its pesticidal crystal proteins[J].Microbiol.Mol.Biol.Rev,1998,62:775—806[8] Baumann L,Broadwell A H,Baumann P.Sequence analysis of the mosquitocidal toxin genes encoding 51.4 and 41.9 kilodalton proteins from Bacillus sphaericus 2362 and 2297[J].J.Bacteriol,1998,170:2045—2050[9] Davidson,E W,Oei C,Meyer M,et al.Interaction of the Bacillus sphaericus mosquito larvicidal proteins[J].Can.J.Microbiol,1990,36:870—878[10] Ben-Dov E,Einav M,Peleg N,et al.Restriction map of the 125-kilobase plasmid of Bacillus thuringiensis subsp.israelensis carrying genes that encode delta-endotoxins active against mosquito larvae[J].Appl.Environ.Microbiol,1996,62:3140—3145[11] Arapinis C,de la Torre F and Szulmajster J.Nucleotide and deduced amino acid sequence of the Bacillus sphaericus 1593 M gene encoding a 51.4 kD polypeptide which acts synergistically with the 42 kD protein for expression of the larvicidal toxin[J].Nucl.Acids.Res,1998,16:7731[12] Sangthongpitag K,Penfold RJ and Delaney SF.Cloning and expression of the Bacillus sphaericus 2362 mosquitocidal genes in a non-toxic unicellular cyanobacterium,Synechococcus PCC 630[J].Appl.Microbiol.Biotechnol,1997,47:379—384[13] Angsuthanasombat C and Panyim S.Biosynthesis of 130-Kilodalton mosquito larvicide in the cyanobacterium Agmennellum quadruplicatum PR-6[J].Appl.Environ.Microbiol,1989,55:2428—2430[14] Chungjatupornchai W.Expression of the mosquitocidal-protein genes of Bacillus thuringiensis subsp.israelensis and the herbicide-resistance gene bar in Synechocystis PCC 6803[J].Curr.Microbiol,1990,21:283—288[15] Murphy RC,and Stevens SE Jr.Cloning and expression of the cryIVD gene of Bacillus thuringiensis subsp.israelensis in the cyanobacterium Agmenellum quadruplicatum PR-6 and its resulting larvicidal activity[J].Appl.Environ.Microbiol,1992,58:1650—1655[16] Soltes-Rak E,Kushner DJ,Williams DD,et al.Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp.strain PCC 7942[J].Appl.Environ.Microbiol,1993,59:2404—2410[17] Soltes-Rak E,Kushner DJ,Williams,DD,et al.Factors regulating cryIVB expression in the cyanobacterium Synechococcus PCC 7942[J].Mol.Gen.Genet,1995,246:301—308[18] Thiery I,Nicolas L,Rippka R,et al.Selection of cyanobacteria isolated from mosquito breeding sites as a potential food source for mosquito larvae[J].Appl.Environ.Microbiol,1991,57:1354—1359[19] 孔任秋,徐旭东,胡玉祥.蚊虫孳生地蓝藻的分离及对蚊幼虫适口性的观察[J].中国寄生虫病防治杂志,1992,5:184—186[20] 徐旭东,孔任秋,胡玉祥.基因工程杀蚊幼蓝藻的研究[J].中国媒介生物学及控制杂志,1993,4:244—247[21] Xu X,Yan G,Kong R,et al.Analysis of expression of the binary toxin protein genes from Bacillus sphaericus in Anabaena and the potential in mosquito control[J].Curr.Microbiol,2000,(in press)[22] 孔任秋,徐旭东,胡玉祥,等.杀蚊幼蛋白基因在丝状固氮蓝藻的表达[C].山东省科学技术协会,山东省自然科学进展.北京:中国科学技术出版社,1993.804—810[23] 阎歌,张晓静,刘相萍,等.转基因蓝藻对不同龄期淡色库蚊幼虫杀灭作用的实验研究[J].中国媒介生物学及控制杂志,1998,9:195—197[24] 阎歌,刘相萍,张晓静,等.基因工程灭蚊幼蓝藻的现场初步观察[J].中国媒介生物学及控制杂志,1996,7:85—88[25] Wu X,Xu X,Ben-dov E,et al.Expression of mosquitocidal Bacillus thuringiensis subsp.israelensis endotoxin genes in the filamentous cyanobacterium Anabaena 7120[R].Third European workshop on the molecular biology of cyanobacteria,Spain;Sevilla,1995,38[26] Wu X,Vennison SJ,Liu H,et al.Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp.israelensis[J].Appl.Environ.Microbiology,1997,63:4971—4975[27] 吕颂雅,刘子铎,戴经元,等,苏云金芽孢杆菌杀蚊基因在鱼腥藻中克隆和表达的初步研究[J].水生生物学报,1999,23:174—178[28] Wolk CP,Vonshak A,Kehoe P,et al.Construction of shuttle vectors capable of conjugative transfer from Fscherichia coli to nitrogen-fixing filamentous cyanobacteria[J].Proc,Natl Acad.Sci.USA,1984,81:1561—1565[29] Elhai J,Vepritsky A,Muro-pastor AM,et al.Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp.strain PCC 7120[J].J.Bacteriol 1997,179:1998—2005