温度和体重对中华绒螯蟹幼蟹饥饿代谢的影响
EFFECTS OF TEMPERATURE AND BODY WEIGHT ON FASTING METABOLISM OF THE JUVENILE ERIOCHEIR SINENSIS
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摘要: 于1998年9月-12月对均重为3.64、1.69和0.2g三种规格的中华绒螯蟹幼蟹在1、20、2和30℃四种温度条件下的饥饿代谢进行了测定,以探讨体重和温度变化对代谢的影响.结果表明,耗氧率(RO)和氨氮排泄率(RN)都随温度升高而增大,随体重增大而减小,其关系式可分别表示为:RO＝0.1309We0.0490T和RO=0.424W-0.1999;RN=3.3649e0.0484T和RN＝0.4400W-2.900.不同体重幼蟹在不同温度条件下的饥饿代谢率差异显著,饥饿代谢率随体重的增大而减少,随温度升高而增大.同时,体重对能源物质的供能比有较大影响,三种规格的幼蟹,其代谢底物中蛋白质、碳水化合物和脂肪提供的能量比分别为6.4:41.8:1.8、6.7:27.8:6.和7.3:43.8:48.9.Abstract: The Chinese mitten-handed crab Eriocheir sinensis, is considered to be a good crustacean species for culture in China. There have been a lot of researches on its breeding, culture, treatment of disease and nutrition last years. But the physio-ecological study has not been reported, especially oxygen consumption data are lacking for Eriocheir sinensis. In this study, the effect of temperature and body weight on the metabolism of the juvenile Eriocheir sinensis (weighing from 0.40g to 5.19g) was conducted during Sept. to Dec., 1998 by normal aquatic chemical methods. A cone flask with 250mL of internal volumes was used as respiratory chamber and was filled with tap water that was dechlorined for 7 days. Dissolved oxygen (DO) levels were determined using the Winkler method and free CO2 quantity was determined by a phenolphthanlein indicator with the NaOH standard solution used. The Nessler reagent method was used for determination of the NH3-N quantity. The fasting metabolic rate of the crabs of three weight groups (3.64, 1.69 and 0.52g) was determined at the temperature of 15, 20, 25 and 30℃ also. The experimental results showed both oxygen consumption (RO, mg/g/h) and excretion rate of ammonia-N (RN, g/g/h) were affected significantly by water temperature (T, ℃) and body weight (W, g). RO and RN increased with increasing temperature and decreased with increasing body weight. The relationships could be expressed as: R O=0.1309e0.0490T (r=0.8391, n=75, p0.01) and RN= 3.3649e0.0484T(r=0.6999, n=75, p0.01); R O=0.4254W-0.1999 (r=-0.5094, n=75, p0.01) and RN= 0.4400W-2.900 (r=-0.6237, n=75, p0.01), respectively. The compound correlation between temperature, weight and ROO-0.2138e 0.0504T (r=0.9876, n=75, P0.01); and the same relationship for RNN-0.3032e0.0502T (r=0.9563, n=75, p0.01). The ratio of oxygen to nitrogen was not significantly affected by temperature (p0.05) but markedly influenced by crab's body weight (p0.01). The fasting metabolic rate also decreased with increasing body weight and increased with increasing temperature. The ratio of metabolic substrate-protein, lipid and carbohydrate at three groups of juvenile crab weighing 3.64, 1.69 and 0.52g were 6.4:41.8:51.8, 6.7:27.8:65.5 and 7.3:43.8:48.9, respectively. The ratio of protein gradually increased with decreasing body weight. But the ratio of metabolic substrate was not regularly affected by temperature.
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Keywords:
- Juvenile Eriocheir sinensis /
- Temperature /
- Body weight /
- Fasting metabolism
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