红拟石首鱼海豚链球菌分离、鉴定及致病性研究

沈智华; 钱冬; 许文军; 顾金华; 邵健忠

红拟石首鱼海豚链球菌分离、鉴定及致病性研究

沈智华^1,2,
钱冬²,
许文军³,
顾金华⁴,
邵健忠^1, ,

浙江大学生命科学学院, 杭州 310012
2. 浙江省淡水水产研究所, 湖州 313001
3. 浙江省海洋水产研究所, 舟山 316100
4. 湖州市中心医院,湖州 313000

基金项目:

浙江省自然科学基金(Y3004425)资助

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- 收稿日期: 2004-03-01
- 修回日期: 2005-05-29
- 发布日期: 2005-11-24

ISOLATION, IDENTIFICATION AND PATHOGENICITY OF STREPTOCOCCUS INIAE ISOLATED FROM RED DRUM SCIAENOPS OCELLATUS

College of Life Sciences, Zhejiang University, Hangzhou 310012;
2. Zhejiang Institute of Freshwater Fisheries, Huzhou 313001;
3. Zhejiang Institute of Marine Fisheries, Zhoushan 316100;
4. Huzhou Central Hospital, Huzhou 313000

摘要

摘要: 2001年9月-12月,浙江舟山部分网箱养殖红拟石首鱼发生了陆续死鱼,发病鱼表现为眼球突出、浑浊,失去方向性,皮肤溃疡等症状。从病鱼的肾脏和肝脏中分离到菌株SO-2和SO-3,对两分离株进行了致病性试验,发现两者对红拟石首鱼、罗非鱼及小白鼠均有致病力,SO-2和SO-3对红拟石首鱼、罗非鱼及小白鼠的LD50分别为4.8×108CFU/尾和1.9×107CFU/尾2、.8×108CFU/尾和8.3×107CFU/尾及9.6×106CFU/只和4.2×106CFU/只,试验感染鱼出现眼球突出、浑浊及失去方向性等与自然发病相似症状,确定该两株菌为致病菌。两分离株为革兰氏染色阳性,呈链状球菌,β溶血,10℃生长,45℃不生长;接触酶阴性,水解七叶灵、精氨酸;VP试验、脲酶和马脲酸试验阴性,发酵葡萄糖、水杨苷、蔗糖和淀粉,不发醇阿拉伯糖、菊糖、乳糖、蜜二糖、棉子糖和山梨醇。分离株对氨卞青霉素、万古霉素和先锋Ⅴ等高度敏感,对庆大霉素、复方新诺明、林可霉素、氟哌酸等不敏感。应用16SrRNA基因进行的菌株PCR鉴定,确定该两株菌为海豚链球菌。
- 红拟石首鱼 /
- 海豚链球菌 /
- 致病性 /
- 鉴定
Abstract: In recent years,red drum(Sciaenops ocellatus) became the economic important species of marine cage culture in Zhejiang Province. During September to October 2001,continuous mortality of cagecultured red drum was recorded in Zhoushan,with the symptoms of skin lesions,exophthalmia and disorientation,etc. Two bacterial strains SO22 and SO23 were isolated fromthe kidney of moribund red drum. The pathogenicity of these two isolates to red drum,tilapia and mouse was tested. The LD50ofSO22 and SO23 to red drum were 418 × 108CFU/fish and 119 × 107CFU/fish,to tilapia 218 × 108CFU/fish and 813×107CFU/fish,to mouse 916×106CFU/ mouse and 412 × 106CFU/ mouse,respectively. The challenged fish presented the similar externalsigns to natural infected red drum,such as exophthalmia and disorientation. These two isolates were preliminarily identified asStreptococcus iniae with the characteristics of gram positive cocci in chains, catalase negative and beta2hemolytic,growth under10 ℃and non growth up 45 ℃,hydrolyzation of esculin and arginine,Voges Proskauer,urease,and hippurate tests negative,fer mentation of glucose,salicin,sucrose and starch,non fermentation of arabinose,inulin,lactose,melibiose,raffinose and sorbitol.They were highly sensitive to ampicilin,vancomycin and cefazolin,while resistant to gentamicin,compound sulfamethoxazole,lincomycin and norfloxacin. Polymerase chain reaction(PCR) of 16SrRNA gene was carried out by using the primers specific forStreptococcus iniae and a 300bp product was identified with 2% agar electrophoresis,which confirmed the identification of thesetwo isolates as Streptococcus iniae.
- Red drum( Sciaenops ocellatus) /
- Pathogenicity /
- Streptococcus iniae /
- Identification

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参考文献(15)

[1]	Chen T S. Preparation and applicationof microbial medium[M].Beijing:Chinese Agriculture Press.1995:93-95[陈天寿. 微生物培养基的制造与应用. 北京:中国农业出版社.1995:93-95]
[2]	Qian D,Chao Z,Shen J Y,et al. The optimum conditions for growthof Aeromonas hydrophilas BSK 210 [J]. Journal of Fishery Sciences ofChina,2001,8(3):38-44[钱冬,曹铮,沈锦玉等. 嗜水气单胞菌BSK 210 株的优化培养及生长动态研究. 中国水产科学,2001,8(3):38-44]
[3]	Zlotkin A,Hershko H,Eldar A. Possible Transmissionof Streptococcusiniae from Wild Fish to Cultured Marine Fish[J]. Appl. Environ. Mi2crobiol,1998;64:4065-4067
[4]	Huang Z X. Basic medico virology and method[M]. Beijing:SciencePress.1990:143-144[黄祯祥. 医学病毒学基础及实验技术. 北京:科学出版社.1990:143-144]
[5]	Y in Z,Xu B H. Studies on the bacteriosis of fishes[J]. Acta Hydrobi ologica Sinica,1995,19(1):76-83[殷战,徐伯亥. 鱼类细菌性疾病的研究. 水生生物学报,1995,19(1):76-83]
[6]	Weinstein M R,Litt M,Kertesz D A,et al. Invasive Infections Due toa Fish Pathogen, Streptococcus iniae[J]. N. Engl. J. Med.,1997,337:589-594
[7]	Eldar A,Bejerano Y,Bercovier H. Streptococcus shiloi and Streptococcus difficile: two new streptococcal species causing a meningoen2cephalitis in fish[J]. Curr. Microbiol.,1994,28:139-143
[8]	Perera R,Johnson S,Collins M, et al. Streptococcus iniae associatedwith mortalityof Tilapia nilotica and T1aurea hybrids[J]. Journal ofAquatic Animal Health,1994,6:335-340
[9]	Pier G,Madin S.Streptococcus iniae sp. nov.,a beta hemolyticstreptococcus isolated from an Amazon fresh water dolphin,Inia geoffrensis[J]. Int. J. Syst. Bacteriol., 1976,26:545-553
[10]	Eldar A,Perl S,Frelier P E,et al. Red drum Sciaenops ocellatus mortalities associated with Streptococcus iniae infection[J]. Dis. Aquat.Org.,1999,36:121-127
[11]	Colorni A,Diamant A,Eldar A,et al. Streptococcus iniae infections inRed Sea cagecultured and wild fishes[J]. Dis. Aquat. Org.,2002,49:165-170
[12]	Weinstein M R,Low D E,McGeer A,et al. Preliminary Report. Invasive infection due to Streptococcus iniae:a newor previously unrecog2nized disease Ontario.1995-96[J]. CCDR,1996,22:129-132
[13]	Sneath P H A, Mair N A,Sharp M E,et al.Bergey’s manual of systematic bacteriology[M]. Baltimore: The Williams & Wikins Co.,1986,2:999-1002
[14]	Berridge B R,Fuller J D,Azavedo J, et al. Development of SpecificNested Oligonucleotide PCR Primers for the Streptococcus iniae16S-23S Ribosomal DNA Intergenic Spacer[M]. Journal of ClinicalMicrobiology,1998,36(9):2778-2781
[15]	G oh S H,Driedger D, G illett S, et al. Streptococcus iniae, a Humanand Animal Pathogen: Specific Identification by the Chaperonin 60Gene Identification Method[J]. JCM.1998,36(3):2164-2166

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红拟石首鱼海豚链球菌分离、鉴定及致病性研究

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出版历程

ISOLATION, IDENTIFICATION AND PATHOGENICITY OF STREPTOCOCCUS INIAE ISOLATED FROM RED DRUM SCIAENOPS OCELLATUS

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出版历程

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