高比例棉粕饲料中补充蛋氨酸对中华绒螯蟹幼蟹摄食、生长及抗氧化酶活性的影响
Effects of dietary methionine supplementation on feeding, growth and antioxidant ability of juvenile Chinese mitten crab, Eriocheir sinensis
-
摘要: 以40%棉粕为主的混合蛋白源制成基础饲料, 分别在其中添加0.00%、0.14%、0.28%、0.42%、0.56%蛋氨酸(分别记为0.00%Met、0.14%Met、0.28%Met、0.42%Met和0.56%Met), 配制了5种等氮等能的试验饲料, 以全鱼粉组(64.4%鱼粉)作为对照, 探讨了在高比例棉粕饲料中补充蛋氨酸对中华绒螯蟹幼蟹(0.390.02) g的摄食率、生长率和机体抗氧化酶活性的影响。结果表明: 与全鱼粉对照组相比, 0.42%Met组的增重率、特定生长率和饲料系数均无显著差异(P0.05), 但其显著高于0.00%Met、0.14%Met和0.28%Met组(P0.05); 增重率和特定生长率则显著高于0.56%Met组(P0.05); 从幼蟹的摄食量和蛋白质沉积率来看, 0.42%Met和0.28%Met组的摄食量与对照组相似, 当蛋氨酸的补充量低于0.28%或高于0.42%时, 幼蟹的摄食量均有所下降; 统计表明, 对照组的蛋白质沉积率最高(23.20%), 在各试验组中, 0.28%Met和0.42%Met组之间的蛋白质沉积率无显著差异(P0.05), 但显著高于0.00%Met、0.14%Met和0.56%Met组(P0.05); 分析幼蟹的肠道胰蛋白酶活性, 发现0.42%Met组与对照组之间无显著性差异(P0.05), 但均显著高于其他各试验组(P0.05); 0.28%Met、0.42%Met和0.56%Met处理组的血清丙二醛含量、超氧化物歧化酶活性和谷胱甘肽过氧化物酶活性与对照组无显著差异(P0.05)。结果提示, 在高比例植物蛋白(40%棉粕)的基础饲料中, 补充0.42%Met能够显著提高中华绒螯蟹幼蟹的生长率、消化率和抗氧化酶的活性。Abstract: This study was conducted to evaluate the effects of dietary methionine supplementation in cottonseed meal- based diets on the growth performance, digestibility and antioxidant ability of juvenile Chinese mitten crab, Eriocheir sinensis. Five diets were formulated using a basic diet, in which cottonseed meal accounted for 40%, supplemented with 0.00%, 0.14%, 0.28%, 0.42%, and 0.56% methionine, respectively. another diet was full fish meal-based (fish meal accounted for 64.4%) and served as the control. Each diet was fed to juvenile E. sinensis [(0.390.02) g] of five replicates for 6 weeks. The results showed that weight gain, specific growth rate and feed conversion ratio in crabs fed 0.42%Met diet did not differ from those of the control group (P0.05). Crabs fed with 0.28%Met and 0.42% Metdiets exhibited higher food intake and protein deposition rate than those fed diets with 0.00%Met, 0.14%Met, 0.56%Met (P0.05). There were no significant differences in whole body moisture, lipid, and ash (P0.05) among all treatments. However, compared with the 0.00% Met diets group, crude protein contents in crab fed with methionine supplemented diets were significantly higher (P0.05). Dry matter, protein, lipid digestibility, and trypsinase activity of crabs fed with 0.42% Metdiets did not differ from those of the control (P0.05). No differences were found in malondiaedehyde content, serum superoxide dismutase and glutathione peroxidase activity in crabs fed with the control diet and those fed with 0.28% Met, 0.42%Met and 0.56% Metdiets (P0.05). The results suggest that 0.42%Met added to the diet (cottonseed meal accounted for 40%) could significantly accelerate the growth performance, digestibility and antioxidant ability of juvenile E. sinensis.
-
Keywords:
- Eriocheir sinensis /
- Methionine /
- Cottonseed meal /
- Feeding /
- Growth /
- Antioxidant
-
-
[1] Du L, Niu C J. Effects of dietary substitution of soya bean meal for fish meal on consumption, growth, and metabolism of juvenile giant freshwater prawn, Macrobrachium rosenbergii [J]. Aquaculture Nutrition, 2003, 9(2): 139-143
[2] Hansen A C, Rosenlund G, Karlsen ?, et al. The inclusion of plant protein in cod diets, its effects on macronutrient digestibility, gut and liver histology and heat shock protein transcription [J]. Aquaculture Research, 2006, 37(8): 773-784
[3] Takea M, Takii K. Fish Chemoreception Gestation and Nutrition in Fishes [M]. London: Hara T J Chapman Hall. 1992, 271-287
[4] Refstie S, Svihus B, Shearer K D, et al. Nutrient digestibility in Atlantic salmon and broiler chickens related to viscosity and non-starch polysaccharide content in different soybean products [J]. Animal Feed Science and Technology, 1999, 79(4): 331-345
[5] Glencross B, Evans D, Rutherford N, et al. The influence of the dietary inclusion of the alkaloid gramine, on rainbow trout (Oncorhynchus mykiss) growth, feed utilization and gastrointestinal histology [J]. Aquaculture, 2006, 253(1-4): 512-522
[6] National Research Council. Nutrient Requirements of Fish [M]. The National Academy of Science. 1993, 6-12
[7] Dabrowski K, Poczyczynski P, Ktick G, et al. Effect of partly or totally replacing fish meal protein by soybean meal protein on growth, food utilization and proteolytie enzyme activities in rainbow trout (Salmo gairdneri)．New in vivo test for exocrine pancreatic secretion [J]. Aquaculture, 1989, 77(1): 29-49
[8] Mukhopadhyay N, Ray A K. Effects of amino acid supplementation on the nutritive quality of fermented linseed meal protein in the diets of rohu, Labeo rohita, fingerlings [J]. Journal of Applied Ichthyology, 2001, 17(5): 220-226
[9] Nose T. Summary Report on the Requirements of Essential Amino Acids for Carp [M]. Berlin: Tiews Heenemann. 1979, 145-156
[10] Ahmed I, Khan M A, Jafri A K. Dietary methionine requirement of fingerling Indian major carp, Cirrhinus mrigala (Hamilton) [J]. Aquaculture International, 2003, 11(5): 449-462
[11] Teshima S, Kanazawa A, Uchiyama Y. Effect of several protein sources and other factors on the growth of Tilapia nilotica [J]. Bulletin of Japanese Society of Scientific Fisheries, 1986, 52(3): 525-530
[12] Yin H F, Han Y. The application of amino acid in aquatic feeds [J]. China Fisheries, 2000, 2: 68-69 [尹海富, 韩英. 单体氨基酸在水产饲料中的应用. 中国水产, 2000, 2: 68-69]
[13] He L J, Mo S J, Shi X J, et al. Study on food attractants for Pseudobargus Fulridraco [J]. Journal of Southwest Agriculture University, 2005, 27(3): 416-419 [何利君, 莫绍橘, 施新建, 等. 黄颡鱼摄饵促进物质的研究. 西南农业大学学报, 2005, 27(3): 416-419]
[14] Ache B W. Chemoreception and Thermoreception [M]. London: Academic Press. 1982, 369-393
[15] Chen L Q, Du N S, Lai W. Evaluation of soybean cake as a substitute for partial fish meal in formulated diets for juvenile Chinese mitten-handed crab [J]. Journal of Fisheries of China, 1994, 18(1): 24-31 [陈立侨, 堵南山, 赖伟. 中华绒螯蟹蟹种配饵中豆饼替代部分鱼粉的适宜含量. 水产学报, 1994, 18(1): 24-31]
[16] Jiang H B, Chen L Q, Li E C, et al. Partial or total replacement of soybean meal by cottonseed meal in practical diets for Chinese mitten crab, Eriocheir sinensis: effects on oxygen consumption, ammonia excretion, O∶N ratio and amino transferases activities [J]. Turkish Journal of Fisheries and Aquatic Sciences, 2012, 12(3): 547-554
[17] Chen L. Effect of Dietary Cottonseed meal level on growth, some physiological and biochemical indices related in juvenile Chinese mitten-handed crab, Eriocheir sinensis [D]. Thesis for Master of Science. East China Normal University, 2010 [陈亮, 不同棉籽粕水平饲料对中华绒螯蟹幼蟹生长和相关生理生化指标的影响. 硕士学位论文, 2010]
[18] AOAC. Official Methods of Analysis: 930.15,930.29,920. 39c [M]. 16th ed. Arlington, VA: AOAC, 1995
[19] Furukawa A, Tsukahara H. On the acid digestion method for determination of chromic oxide as index substance in the study of digestibility in fish feed [J]. Bulletin of Japanese Society of Fishers Science, 1996, 32(6): 502-506
[20] Zeng D, Yang C G. Feeding chemoreceptor of aquatic animal and the development and application of aquatic attractant [J]. China Feed, 2002, 23: 15-17 [曾端, 杨春贵. 水产动物的摄食化学感受器及水产诱食剂的开发应用. 中国饲料, 2002, 23: 15-17]
[21] Robinson E H, Li M H. Use of plant proteins in catfish feeds: replacement of soybean meal with cottonseed meal and replacement of fish meal with soybean meal and cottonseed meal [J]. Journal of the World Aquaculture Society, 1994, 25(2): 271-276
[22] Mukhopadhyay N, Ray A K. Improvement of quality of sesame (Seasamum indicum) seed meal protein with supplemental amino acids in feeds for rohu, Labeo rohita (Hamilton) fingerlings [J]. Aquaculture Research, 1999, 30(8): 549-557
[23] Ye J Y, Guo J L. Lysine, methionine and arginine requirements of juvenile Chinese mitten crab, Eriocheir sinensis [A]. The Seventh Symposium Worlds Chinese Scientists on Nutrition and Feeding of Finfish Shellfish (Abstracts) [C]. Beijing. 2008, 43 [叶金云, 郭建林. 中华绒螯蟹(Eriocheir sinensis)幼蟹对赖氨酸, 蛋氨酸及精氨酸的需要量研究. 第七届世界华人鱼虾营养大会(摘要集). 北京. 2008, 43]
[24] Schwarz F J, Manfred K, Ute D. Studies on the methionine requirement of carp (Cyprinus carpio L.) [J]. Aquaculture, 1998, 161(124): 121-129
[25] Lin S M, Mai K S, Tan B P. Influence of practical diet supplementation with free D-methionine on the growth and body composition in Tilapia Oreochromis Niloticus O. Aureus [J]. Acta Hydrobiologica Sinica, 2008, 32(5): 741-749 [林仕梅, 麦康森, 谭北平. 实用饲料中添加结晶蛋氨酸对罗非鱼生长和体组成的影响. 水生生物学报, 2008, 32(5): 741-749]
[26] Carter C G. Protein synthesis in grass carp, Ctenop haryngodon idella, and its relation to diet quality, Fish nutrition in practice: 4th international symposium on fish nutrition and feeding. Biarritz, France, June 24-27, 1991 (edited by kaushik S J, Luquet). 1993, 673-680
[27] Md S A, Shin-ichi T, Shunsuke K, et al. Supplemental effects of coated methionine and/or lysine to soy protein isolate diet for juvenile kuruma shrimp. Marsupenaeus japonicus [J]. Aquaculture, 2005, 248(1-4), 13-19
[28] Nordrum S, Krogdahlb S R, Nordrnm S, et al. Effects of methionine, cysteine and medium chain triglycerides on nutrient digestibility, absorption of amino acids along the intestinal tract and nutrient retention in Atlantic salmon (Salmo salar L.) under pair feeding regime [J]. Aquaculture, 2000, 186(3-4): 341-360
[29] Lin S M, Luo L, Ye Y T, et al. Effects of dietary protein to energy ratio and N-Phy-P levels on nitrogen and phosphorus excretion and activities of GOT and GPT in Eriocheir sinensis [J]. Journal of Fishery Sciences of China, 2001, 8(4): 62-66 [林仕梅, 罗莉, 叶元土, 等. 饲料蛋白能量比、非植酸磷水平对中华绒螫蟹氮、磷排泄和转氨酶活力的影响. 中国水产科学, 2001, 8(4): 62-66]
[30] Chi S Y, Lin H Z, Tan B P, et al. Effects of microcapsules or crystalline methionine in lower fishmeal diets on digestive enzyme activity of Pacific White Shrimp (Litopenaeus vannamei) [J]. Modern Agricultural Sciences and Technology, 2010, 11: 308-310 [迟淑艳, 林黑着, 谭北平, 等. 低鱼粉饲料中添加微胶囊或晶体蛋氨酸对凡纳滨对虾消化酶活性的影响. 现代农业科技, 2010, 11: 308-310]
[31] Ana F, Venturin O, Ana M. Effects of carbaryl and azinphos methyl on juvenile rainbow trout (Oncorhynchus mykiss) detoxifying enzymes [J]. Pesticide Biochemistry and Physiology, 2007, 88(2): 134-142
[32] Xiao W W, Feng L, Liu Y, et al. A comparative study of feeding effects of equal-sulfur supplementation with DL-methionine and methionine hydroxy analogue free acid in juvenile Jian Carp (Cyprinus carpio var. Jian) [J]. Chinese Journal of Animal Nutrition, 2010, 22(4): 1122-1130 [肖伟伟, 冯琳, 刘扬, 等. 日粮中等硫添加DL-蛋氨酸和蛋氨酸羟基类似物游离酸在幼建鲤上饲喂效果的比较研究. 动物营养学报, 2010, 22(4): 1122-1130]
[33] Cheng Z J, Hardy R W, Blair M. Effects of supplementing methionine hydroxy analogue in soybean meal and distillers dried grain-based diets on the performance and nutrient retention of rainbow trout (Oncorhynchus mykiss, Walbaum) [J]. Aquaculture Research, 2003, 34(14): 1303-1310
-
期刊类型引用(19)
1. 魏正玲，罗凯，邵仙萍，郜卫华，吴成龙，赵成民. 菜籽粕替代鱼粉对青鱼生长、抗氧化能力和肠道健康的影响. 动物营养学报. 2024(06): 3876-3891 . 
百度学术
2. 陈龙，刘小玲，阚画春，王维，郑曙峰，李淑英，陈敏，陈超，徐道青. 棉粕饲料化高效利用分析. 安徽农学通报. 2024(12): 68-73 . 百度学术
3. 陈倪慧，杨霞，孙珊珊，雷西汀，邵仙萍，吴成龙，叶金云. 发酵植物蛋白复配替代豆粕对青鱼生长、免疫、抗氧化及肠道菌群结构的影响. 动物营养学报. 2023(04): 2478-2489 . 百度学术
4. 付豪，梁艺馨，牟希东，刘超，杨叶欣，刘奕，宋红梅. 发酵南极磷虾粉对锦鲤生长性能、血清生化指标、肠道结构及菌群的影响. 广东海洋大学学报. 2023(05): 34-42 . 百度学术
5. 王冰柯，张芹，王延晖. 饲料蛋白质水平对水产动物生长及抗氧化的影响. 河南水产. 2021(04): 3-5 . 百度学术
6. 苏美珍，张祎桐，王浩，吕利群. 甲苯咪唑在异育银鲫体内代谢及肝毒性研究. 水产科学. 2020(05): 710-718 . 百度学术
7. 申建飞，陈铭灿，刘泓宇，谭北平，董晓慧，杨奇慧，迟淑艳，章双，王华朗. 浓缩棉籽蛋白替代鱼粉对卵形鲳鲹幼鱼生长性能、血清生化指标、肝脏抗氧化指标及胃肠道蛋白酶活性的影响. 动物营养学报. 2019(02): 746-756 . 百度学术
8. 周凡，贝亦江，李诗言，王鼎南，丁雪燕，周万华. 不同蛋氨酸剂型对中华鳖生长性能和肝脏基因表达的影响. 水产科技情报. 2019(02): 73-80 . 百度学术
9. 孙金秋，徐莞媛，高焕，阎斌伦. 中华绒螯蟹营养生理学研究进展. 水产科技情报. 2018(04): 197-202 . 百度学术
10. 张海涛，蒲雪松，杨奇慧，谭北平，董晓慧，迟淑艳，刘泓宇，章双. 高蛋白棉粕替代鱼粉对凡纳滨对虾生长性能、非特异性免疫指标及抗病力的影响. 广东海洋大学学报. 2018(04): 20-26 . 百度学术
11. 于海馨，徐东坡，周游，游磊，杨彦平，刘凯. 中华绒螯蟹生殖群体生物学特征及消化酶活力研究. 上海海洋大学学报. 2018(06): 844-854 . 百度学术
12. 何娇娇，王萍，冯建，娄宇栋，党慧，邓蓉. 玉米蛋白粉替代鱼粉对大黄鱼生长、血清生化指标及肝脏组织学的影响. 水生生物学报. 2017(03): 506-515 . 本站查看
13. 崔燕燕，张南南，马倩倩，陈晴，沈振华，杜震宇，陈立侨. 四种植物蛋白对中华绒螯蟹幼蟹生长性能、氨基酸沉积率和抗氧化酶活性的影响. 水生生物学报. 2017(01): 146-154 . 本站查看
14. 姚清华，颜孙安，郭清雄，王宝珍，陆鹏，林虬. 羽毛肽粉对瓦氏黄颡鱼稚鱼生长、体成分和消化酶活性的影响. 福建农业学报. 2016(06): 580-585 . 百度学术
15. 向枭，周兴华，曾本和，罗莉，文华. 蛋氨酸水平对吉富罗非鱼肌肉氨基酸组成及血清抗氧化能力的影响. 水产学报. 2016(09): 1359-1367 . 百度学术
16. 杨霞，叶金云，张易祥，吴成龙，刘沛，王伟. 普通棉籽粕和发酵棉籽粕替代鱼粉对中华绒螯蟹幼蟹生长性能、体成分及肝胰腺消化酶活性的影响. 动物营养学报. 2014(03): 683-693 . 百度学术
17. 徐奕晴，祝向阳，颜培实. 生物发酵饲料对中华绒螯蟹幼蟹生长、饲料利用及抗氧化酶活性的影响. 江苏农业科学. 2014(07): 237-240 . 百度学术
18. 严全根，朱晓鸣，杨云霞，韩冬，金俊琰，解绶启，李钟杰. 饲料中棉粕替代鱼粉蛋白对草鱼的生长、血液生理指标和鱼体组成的影响. 水生生物学报. 2014(02): 362-369 . 本站查看
19. 杨霞，叶金云，周志金，张易祥，吴成龙，明建华. 中华绒螯蟹幼蟹对亮氨酸和异亮氨酸的需要量. 水生生物学报. 2014(06): 1062-1070 . 本站查看
其他类型引用(29)
计量
- 文章访问数: 1376
- HTML全文浏览量: 2
- PDF下载量: 835
- 被引次数: 48
下载: