Abstract: The characteristics of three kinds of determination materials of Takifugu obscurusAbe,otoliths,vertebra and opercular had been described and compared the similarities and difference and veracity As a result,the otoliths are the best structure of judging age because the annual ring is very evidence and regularity;the vertebra is the better,it is difficultyof measure ;the opercular is only the assistant material1Among the 72 specimens selected to identify the age of fish using three structures,opercular and otoliths provided respectively the lowest (n = 72,62.50 % ) and highest (n = 72,98.61 % ) number of interpretable preparations1Observed ages rangedfrom age 1+to 4+usingotoliths and vertebra,1+to 3+usingopercular1The teeth of Takifugu obscurus Abe also were used for judging the age and we have found out some preparatory result.